Use for nutritional assessment of vitamin B1 (thiamine).
Performed
Sun-Sat
Department
Sendout
Reported
2-5 days
Synonyms
B1 Vitamin
TDP
Thiamin Pyrophosphate
Thiamine
Thiamine Diphosphate
TPP
VITB1
Thiamine Pyrophosphate
Notes
Whole blood is the preferred specimen for thiamine assessment. Approximately 80 percent of thiamine present in whole blood is found in red blood cells.
Preferred Specimen Container
Lavender
Collect
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).
Specimen Preparation
Transfer 3 mL whole blood to an ARUP standard transport tube (Min: 0.6 mL) and freeze within 1 hour of collection.
Unacceptable Conditions
Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or nonfrozen specimens.
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Transport in a FROZEN ARUP Screw Top Standard Transport Tube.
This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.
Methodology
Quantitative High Performance Liquid Chromatography (HPLC)/Liquid Chromatography-Tandem Mass Spectrometry
CPT Codes
84425
LOINC
74444-1
ARUP Test Code
0080388
Ordering
Ordering Recommendations
Use for nutritional assessment of vitamin B1 (thiamine).
Performed
Sun-Sat
Department
Sendout
Reported
2-5 days
Synonyms
B1 Vitamin
TDP
Thiamin Pyrophosphate
Thiamine
Thiamine Diphosphate
TPP
VITB1
Thiamine Pyrophosphate
Notes
Whole blood is the preferred specimen for thiamine assessment. Approximately 80 percent of thiamine present in whole blood is found in red blood cells.
Collect
Preferred Specimen Container
Lavender
Collect
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).
Specimen Preparation
Transfer 3 mL whole blood to an ARUP standard transport tube (Min: 0.6 mL) and freeze within 1 hour of collection.
Unacceptable Conditions
Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or nonfrozen specimens.
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Transport in a FROZEN ARUP Screw Top Standard Transport Tube.
This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.
Methodology
Quantitative High Performance Liquid Chromatography (HPLC)/Liquid Chromatography-Tandem Mass Spectrometry
Laboratories.