Samples anticoagulated with heparin are unsuitable for this assay.
Plasma not separated from whole blood within 24 hours of the collection time.
Expired collection tubes.
Collection Comments
Separate from cells within 24 hours of collection. Transfer 2 mL plasma to a sterile aliquot tube.
BKV primary infection occurs mainly during childhood and is usually asymptomatic. The seroprevalence in adults is up to 90%. After primary infection, BKV remains latent in kidney cells and can be reactivated under immune deficiency conditions, such as transplantation. BKV infection can be correlated with tubulointerstitial nephritis and ureteric stenosis in renal transplant recipients as well as hemorrhagic cystitis in bone marrow transplant recipients. It has also been associated with disease patterns for vasculopathy, pneumonitis, encephalitis, retinitis, and even multi-organ failure. Persistent high-level BKV replication is the typical characteristic of polyomavirus- associated nephropathy (PAN) in renal transplantation patients. Up to 5% of renal allograft recipients can be affected about 40 weeks post-transplantation. The presence of DNA in plasma in conjunction with the persistence of nephropathy, and its disappearance from plasma after the reduction of immunosuppressive therapy. Clinically relevant infections are mostly limited to immunosuppressed individuals. The linear range of the BK Plasma assay is 21.5-100,000,000 IU/mL.
Performed
Monday - Friday
Methodology
Quantitative; RT-PCR
Reported
1-3 days
Performing Lab
Molecular
Synonyms
BK Virus PCR QN Pl
11976
BKV, Bld QN
BK Virus QN PCR
BKQN
Reference Interval
Detected Range: 21.5 - 100,000,000 IU/mL
Reference Interval: Not Detected
Assay Limitation(s)
Reliable results depend on proper sample collection, storage and handling procedures.
This test has been validated only for use with EDTA plasma and cobas PCR Media Stabilized urine. Testing of other sample types may result in inaccurate results. Plasma viral load measurements are not directly comparable to those of other sample types.
As with any molecular test, mutations within the target regions of BKV could affect primer and/or probe binding resulting in the under-quantitation of virus or failure to detect the presence of virus.
CPT Codes
87799
LOINC Codes
NAME
LOINC
Result
BK DNA Pl QN
32284-2
BK DNA Pl Log 10
44805-0
Test Code (Outreach Synonym)
11976
Catalog Code
10811955779
Collection
Collect
Blood, Lavender
Preferred Draw Volume
6 mL
Minimum Draw Volume
2.5 mL
Pediatric Collection
3 Lavender microtainers
Unacceptable Conditions
Samples anticoagulated with heparin are unsuitable for this assay.
Plasma not separated from whole blood within 24 hours of the collection time.
Expired collection tubes.
Collection Comments
Separate from cells within 24 hours of collection. Transfer 2 mL plasma to a sterile aliquot tube.
BKV primary infection occurs mainly during childhood and is usually asymptomatic. The seroprevalence in adults is up to 90%. After primary infection, BKV remains latent in kidney cells and can be reactivated under immune deficiency conditions, such as transplantation. BKV infection can be correlated with tubulointerstitial nephritis and ureteric stenosis in renal transplant recipients as well as hemorrhagic cystitis in bone marrow transplant recipients. It has also been associated with disease patterns for vasculopathy, pneumonitis, encephalitis, retinitis, and even multi-organ failure. Persistent high-level BKV replication is the typical characteristic of polyomavirus- associated nephropathy (PAN) in renal transplantation patients. Up to 5% of renal allograft recipients can be affected about 40 weeks post-transplantation. The presence of DNA in plasma in conjunction with the persistence of nephropathy, and its disappearance from plasma after the reduction of immunosuppressive therapy. Clinically relevant infections are mostly limited to immunosuppressed individuals. The linear range of the BK Plasma assay is 21.5-100,000,000 IU/mL.
Performed
Monday - Friday
Methodology
Quantitative; RT-PCR
Reported
1-3 days
Performing Lab
Molecular
Synonyms
BK Virus PCR QN Pl
11976
BKV, Bld QN
BK Virus QN PCR
BKQN
Result Interpretation
Reference Interval
Detected Range: 21.5 - 100,000,000 IU/mL
Reference Interval: Not Detected
Assay Limitation(s)
Reliable results depend on proper sample collection, storage and handling procedures.
This test has been validated only for use with EDTA plasma and cobas PCR Media Stabilized urine. Testing of other sample types may result in inaccurate results. Plasma viral load measurements are not directly comparable to those of other sample types.
As with any molecular test, mutations within the target regions of BKV could affect primer and/or probe binding resulting in the under-quantitation of virus or failure to detect the presence of virus.
