Blood, Lt Blue (x5), Gold, Lt Green, AND Lavender (x2). See Collection Comments for complete collection directions.
Preferred Draw Volume
Lt Green 4.5 mL, Lt Blue 2.7 mL (x5), Lavender 3 mL (x2), Gold 5 mL
Unacceptable Conditions
Blue Tube: Whole blood or plasma on ice, clotted sample, sample collected in glass tube, unspun specimen > 4 hours old, underfilled tube, or gross hemolysis.
Gold Tube: Avoid hemolyzed and lipemic specimens.
Lt Green Tube: Samples thawed more than once or serum.
Lavender Tube: Clotted or contaminated sample or if collected in any other anticoagulant.
Collection Comments
Collect 1 Clear Discard, 5 Full 2.7 mL plastic Blue, 1 Gold, 1 Lt Green, and 2 Lavender in order left to right. Processing: Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hours of collection: Spin all five Blue tubes at 3000rpm for 15 min. Place top 2/3 of plasma in 5 aliquot tubes and spin aliquots at 3000 rpm for 15 min. Take top 2/3 of 2nd spin and put into 7 aliquot tubes. Mark aliquot tubes 'plasma'. Freeze all 7 aliquots immediately. Send 7 frozen aliquots of platelet poor plasma. Also send refrigerated RBC's from one Blue tube. Gold tubes - Spin and separate ASAP. No severely lipemic, contaminated, or hemolyzed specimens. Send 2 aliquots of refrigerated serum. Lt Green - Spin, separate and freeze plasma within 1 hour of collection. Mark tube 'plasma'. Send frozen plasma. Lavender - Do Not Spin. Send refrigerated whole blood. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Frozen plasma, refrigerated serum, RBC's and whole blood
Ordering Recommendations
Careset includes: APC Resistance (APCR), PT Gene Mutation, AT III Functional, Factor VIII Assay, Protein C Activity and Protein S Antigen Free (if patient is not on Coumadin), Homocysteine, Lupus Anticoagulant (DRVVT and StaClot LA), Cardiolipin Ab IgG/M, Beta 2 Glycoprotein 1 IgG/M Ab. Reflex testing for Factor V Leiden PCR will be performed if APCR is abnormal. A MTHFR Mutation Bld will be performed if homocysteine is abnormal.
Blood, Lt Blue (x5), Gold, Lt Green, AND Lavender (x2). See Collection Comments for complete collection directions.
Preferred Draw Volume
Lt Green 4.5 mL, Lt Blue 2.7 mL (x5), Lavender 3 mL (x2), Gold 5 mL
Unacceptable Conditions
Blue Tube: Whole blood or plasma on ice, clotted sample, sample collected in glass tube, unspun specimen > 4 hours old, underfilled tube, or gross hemolysis.
Gold Tube: Avoid hemolyzed and lipemic specimens.
Lt Green Tube: Samples thawed more than once or serum.
Lavender Tube: Clotted or contaminated sample or if collected in any other anticoagulant.
Collection Comments
Collect 1 Clear Discard, 5 Full 2.7 mL plastic Blue, 1 Gold, 1 Lt Green, and 2 Lavender in order left to right. Processing: Do not send whole blood or plasma to lab on ice. Double spin and freeze plasma within 4 hours of collection: Spin all five Blue tubes at 3000rpm for 15 min. Place top 2/3 of plasma in 5 aliquot tubes and spin aliquots at 3000 rpm for 15 min. Take top 2/3 of 2nd spin and put into 7 aliquot tubes. Mark aliquot tubes 'plasma'. Freeze all 7 aliquots immediately. Send 7 frozen aliquots of platelet poor plasma. Also send refrigerated RBC's from one Blue tube. Gold tubes - Spin and separate ASAP. No severely lipemic, contaminated, or hemolyzed specimens. Send 2 aliquots of refrigerated serum. Lt Green - Spin, separate and freeze plasma within 1 hour of collection. Mark tube 'plasma'. Send frozen plasma. Lavender - Do Not Spin. Send refrigerated whole blood. Remember to properly label the aliquot tube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Frozen plasma, refrigerated serum, RBC's and whole blood
Ordering
Ordering Recommendations
Careset includes: APC Resistance (APCR), PT Gene Mutation, AT III Functional, Factor VIII Assay, Protein C Activity and Protein S Antigen Free (if patient is not on Coumadin), Homocysteine, Lupus Anticoagulant (DRVVT and StaClot LA), Cardiolipin Ab IgG/M, Beta 2 Glycoprotein 1 IgG/M Ab. Reflex testing for Factor V Leiden PCR will be performed if APCR is abnormal. A MTHFR Mutation Bld will be performed if homocysteine is abnormal.
