A monoclonal peak with no prior data reflexes to immunofixation. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. Minimum volume for ELP is 0.5 mL serum; for IFE is additional 0.5 mL serum.
Abnormal results will reflex to immunfixation upon pathologists interpretation. The Protein Elp Ser IFE Rflx test consists of a serum protein electrophoresis (SPE) test followed by an immunofixation procedure (IFE) when the pathologist reviewing the initial SPE believes there is a monoclonal band, or a possible monoclonal band, present in the SPE pattern. The results of this test include the pathologist's interpretation of the SPE and when indicated, also the IFE. Note: It is a Medicare requirement that this test must be ordered if the requesting physician would like to have a reflex IFE performed.
Performed
Monday -Friday; Days
Methodology
Electrophoresis/Immunofixation
Reported
2-4 days
Performing Lab
Special Chemistry
Synonyms
6400
Electrophoresis Protein Serum w IFE Rflx
Protein Electrophoresis Serum w IFE Rflx
SPEP w IFE Rflx
SPElpIFE
Protein Electrophoresis Serum with Rflx to Immunofixation
Monoclonal Bands
Reference Chart
CPT Codes
84165, 84155, if reflx add 86334, 82784 (x3), 83883 (x2)
LOINC Codes
NAME
LOINC
Order
Protein Electrophoresis Serum w IFE Rflx
24351-9
Result
SPE 2nd M Protein
35559-4
SPE Albumin
2862-1
SPE Alpha-1
2865-4
SPE Alpha-2
2868-8
SPE Beta
2871-2
SPE Gamma
2874-6
SPE Interp
12851-2
SPE M Protein
33358-3
SPE Total Protein SerPl QN
2885-2
Test Code (Outreach Synonym)
6400
Catalog Code
940069991
Collection
Collect
Blood, Gold
Preferred Draw Volume
3.5 mL
Minimum Draw Volume
3 mL
Other Acceptable Containers
Red
Unacceptable Conditions
No plasma
Collection Comments
A monoclonal peak with no prior data reflexes to immunofixation. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly label tube with patient name, DOB, test and collect date. Minimum volume for ELP is 0.5 mL serum; for IFE is additional 0.5 mL serum.
Abnormal results will reflex to immunfixation upon pathologists interpretation. The Protein Elp Ser IFE Rflx test consists of a serum protein electrophoresis (SPE) test followed by an immunofixation procedure (IFE) when the pathologist reviewing the initial SPE believes there is a monoclonal band, or a possible monoclonal band, present in the SPE pattern. The results of this test include the pathologist's interpretation of the SPE and when indicated, also the IFE. Note: It is a Medicare requirement that this test must be ordered if the requesting physician would like to have a reflex IFE performed.
Performed
Monday -Friday; Days
Methodology
Electrophoresis/Immunofixation
Reported
2-4 days
Performing Lab
Special Chemistry
Synonyms
6400
Electrophoresis Protein Serum w IFE Rflx
Protein Electrophoresis Serum w IFE Rflx
SPEP w IFE Rflx
SPElpIFE
Protein Electrophoresis Serum with Rflx to Immunofixation
Monoclonal Bands
Result Interpretation
Reference Chart
Additional Information
CPT Codes
84165, 84155, if reflx add 86334, 82784 (x3), 83883 (x2)
