Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly labeltube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Refrigerated: 3 days; Frozen: > 3 days at -20°
Ordering Recommendations
The Mitochondrial Antibody test is an indirect fluorescent antibody technique for the screening and semi-quantitative detection of anti-mitochondrial antibodies in human serum. This test uses the NOVA Lite KSL tissue slides which employ a substrate of optimally fixed mouse kidney, stomach, and liver sections. This test is most frequently ordered during an evaluation for primary biliary cirrhosis.
Samples containing mitochondrial antibodies should give a positive result (titer >1:20). Samples that do not have detectable mitochondrial antibodies should give a negative result (titer <1:20). High levels of AMA are often associated with primary billiary cirrhosis. Low titers of AMA may be detected in other liver disorders such as chronic active hepatitis and cryptogenic cirrhosis. About 25% of patients with chronic active hepatitis may demonstrate low AMA titers. The presence of antimitochondrial antibodies is in no way related to the duration of symptoms, suggesting that the antibody appears early in the course of the disease and persists.
Performed
Monday - Friday; Days
Methodology
Immunofluorescence
Reported
2-5 days
Performing Lab
Serology
Synonyms
4817
AMA
MITOCHON
Mitochondrial Ab IF QN
Mitochondrial Antibodies, IF QN
Antimitochondrial
Reference Interval
<1:20
CPT Codes
86256
LOINC Codes
NAME
LOINC
Order
Mitochondrl Antibody IF Quantitative
5247-2
Result
Mitochon Ab IF
5247-2
Mitochon Ab IF Interp
17284-1
Test Code (Outreach Synonym)
4817
Catalog Code
693610
Collection
Collect
Blood, Gold
Preferred Draw Volume
2 mL
Minimum Draw Volume
1 mL
Other Acceptable Containers
Red
Pediatric Collection
2 microtainers
Collection Comments
Do not collect in Orange Tube. Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed angle to separate cells from serum. Refrigerate spun tube. Remember to properly labeltube with patient name, DOB, test and collect date.
Storage/Transport Temperature
Refrigerated serum
Stability (from collection to initiation)
Refrigerated: 3 days; Frozen: > 3 days at -20°
Ordering
Ordering Recommendations
The Mitochondrial Antibody test is an indirect fluorescent antibody technique for the screening and semi-quantitative detection of anti-mitochondrial antibodies in human serum. This test uses the NOVA Lite KSL tissue slides which employ a substrate of optimally fixed mouse kidney, stomach, and liver sections. This test is most frequently ordered during an evaluation for primary biliary cirrhosis.
Samples containing mitochondrial antibodies should give a positive result (titer >1:20). Samples that do not have detectable mitochondrial antibodies should give a negative result (titer <1:20). High levels of AMA are often associated with primary billiary cirrhosis. Low titers of AMA may be detected in other liver disorders such as chronic active hepatitis and cryptogenic cirrhosis. About 25% of patients with chronic active hepatitis may demonstrate low AMA titers. The presence of antimitochondrial antibodies is in no way related to the duration of symptoms, suggesting that the antibody appears early in the course of the disease and persists.
