Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed-angle centrifuge to separate cells from serum. If collecting in a red-top tube, allow the specimen to clot and centrifuge as before, but transfer serum or plasma to an aliquot tube. Remember to properly label tube with patient name, DOB, test and collect date.
Preferred test for individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing Vitamin D insufficiency.
Performed
Monday - Friday; Days
Methodology
Chemiluminescence Immunoassay
Reported
1-7 days
Performing Lab
Chemistry
Synonyms
1,25 Dihydroxyvitamin D GP
7598
Reference Interval
19.9 - 79.3 pg/mL
CPT Codes
82652
LOINC Codes
NAME
LOINC
Order
1,25 Dihydroxyvitamin D
1649-3
Result
1,25 Dihydroxyvitamin D
1649-3
Test Code (Outreach Synonym)
7598
Catalog Code
3572812969
Collection
Collect
Blood, Gold
Preferred Draw Volume
3.5 mL
Minimum Draw Volume
1.2 mL
Other Acceptable Containers
Red, Lt Green
Pediatric Collection
2 microtainers
Unacceptable Conditions
Grossly hemolyzed or lipemic specimens
Collection Comments
Allow Gold tube to sit for 30 minutes to form clot. Spin for 10 minutes at 1100-1300 g in a swing bucket unit or 15 minutes in a fixed-angle centrifuge to separate cells from serum. If collecting in a red-top tube, allow the specimen to clot and centrifuge as before, but transfer serum or plasma to an aliquot tube. Remember to properly label tube with patient name, DOB, test and collect date.
Preferred test for individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing Vitamin D insufficiency.
