Dayton Children's Hospital Laboratory Test Directory

TEST ANNOUNCEMENTS

10/01/2024: Urgent testing update for Mycoplasma pneumoniae.
Due to the recent surge in M. pneumoniae concerns, Dayton Children’s Hospital Laboratory Services has an important update regarding testing availability with education to support this test. The following options are currently available:

DCH in house testing M. pneumoniae is only available within the RIDPC panel	DCH send out testing Available as an independent test by our reference lab partner
TAT <24 hours	TAT 2-5 days
Order: RIDPC (panel)	Order: MPPCR (independent test)
FDA approved	Lab developed test (PCR)
CPT: 0202U	CPT: 87581
DCH Charge: $613.27	DCH Charge: $268.14

Due to low test volumes and required FDA approval, DCH does not have an individual laboratory developed test for Mycoplasma pneumonia. The following tests are included in the RIDPC panel:

Adenovirus
Chlamydia pneumoniae
Coronavirus (HKU1, OC43, NL63)
Influenza A (H1-2009, H1 seasonal, H3)
Influenza B
Human Metapneumovirus (hMPV)
Mycoplasma pneumoniae
Parainfluenza (1,2,3,4)
Rhinovirus/Enterovirus
Respiratory syncytial virus, or RSV
SARS-CoV2

09/30/24: All Positive C. difficile PCR assays will be confirmed by Cytotoxin Assay.
At the request of the Laboratory Stewardship Committee, Infectious Disease Department, and Gastroenterology Department, the Molecular Infectious Disease Laboratory will confirm all positive C. difficile PCR results with a Cytotoxin Assay. This change will take advantage of the Sensitivity of PCR combined with the Specificity of a Toxin Assay. Negative PCRs will be reported immediately, and Positive PCRs will be reported with the Cytotoxin assay result within 24-48 hours. Tests will be resulted as:

Situation	Results
PCR Negative	Negative for C. difficile by PCR
PCR Positive & Cytotoxin Positive	C. DIFFICILE PCR POSITIVE, CYTOTOXIN POSITIVE Patient most likely has disease if signs/symptoms of C. difficile infection are present
PCR Positive & Cytotoxin Negative	C. DIFFICILE PCR POSITIVE, Cytotoxin Negative Patient most likely colonized
PCR Positive & Cytotoxin is pending, a preliminary report will be generated:	Confirmation by Cytotoxin assay to follow in 24-48 hrs

Please contact the Molecular Infectious Disease Laboratory (937-641-3907) with any questions.

09/27/24: A result for EBV Early AB (EA) will no longer be reported with results of Epstein Barr Antibody Evaluation testing.
Testing for EA is not required to assess for primary EBV-associated infection, and EA titers are falsely positive in 10-20% of cases. EA will stay positive in 20% of healthy people with a remote history (i.e. > 6 months prior to testing) of EBV infection. For severely immunocompromised individuals with a documented history of EBV infection confirmed by prior EBV Antibody Evaluation testing results, a positive EA antibody titer result could potentially be used to help assess for EBV infection reactivation. For such cases, please contact the DCH Virology Laboratory for additional information.

TEST MENU CHANGES

(previous month)

Tests not performed at Dayton Children's Hospital are sent to a referral laboratory.

Outside links to referral laboratory order forms should not be utilized; please order tests directly through DCH, and we will facilitate the process.

Genetic testing requires a referral to Genetics.

Laboratory Stewardship aims to ensure that proper tests are ordered, that the retrieval and interpretation of laboratory results is accurate, and that financial responsibility is maintained.

Contact the Laboratory Stewardship Coordinator:

Leslie Willis, BS, MB(ASCP)^CM
937-641-3116

CLIA Certificates for all testing center locations are available upon request.