EPIC Code

LAB6320 GI PCR-11 & GIPCRCULT
LAB2005 UHGIPCR & GIPCRCULT

UCHealth IP/OP orders must be placed through the Epic GI/Diarrhea Wizard.

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics and Microbiology

Collect

Preferred: Stool in a Cary Blair Kit (dark green cap vial & white cap vial).
Acceptable: Stool in a Sterile Container. Stool in appropriately-filled Cary Blair alternative transport media with disclaimer (e.g., Cardinal Health™ C&S (orange cap vial), Cardinal Health™ Enteric Stool Transport Vial (lime green capped vial), or COPAN FecalSwab®).
Minimum volume: 0.5 mL of raw or preserved stool.

Patient Preparation

Specimens containing interfering substances (e.g., castor oil, bismuth, barium, etc.) may not have optimal yield for culture.

Specimen Preparation

Fresh stool added to Cary Blair Transport Kit (dark green & white caps) filled to the fill lines on the labels.

Outpatient Collection Note: Raw stool is only stable for 24 hours refrigerated. Stool received in appropriately-filled Cary Blair Transport Media is stable for 3 days.

Unacceptable Conditions

  • Formed stool.
  • Stool contaminated with urine.
  • Stool in diapers, tissue paper, or homemade containers.
  • Rectal swabs.
  • Frozen stool.
  • Stool not preserved in Cary Blair within 24 hours of collection.
  • Over- or under-filled Cary Blair submitted without at least 0.5 mL of stool in a non-additive, sterile container.
  • Samples from patients who have had a GI PCR <7 days ago.
  • Samples from hospitalized patients admitted >3 days.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.

Storage/Transport Temperature

Release and transport orders/labels for PCR ANDCulture together ("UHGIPCR"/"GI PCR-11" & “GIPCRCULT”).

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Stabilize and/or send to lab ASAP refrigerated.

Performed

Daily

Stability (from collection to initiation)

Room Temp/Refrigerated: 3 days in appropriately-filled Cary Blair (dark green vial); 24 hours in non-additive Sterile Container (white cap vial).
Frozen: Unacceptable.

Notes

Inpatient and ED ordering must occur through the IP GI Wizard. Restrictions apply to adults ≥18 years, with GI PCR Panel only available during the first 2 days of admission. C. diff Toxin EIA will only reflex from a GI Panel when C. diff A/B Gene is detected on patients admitted longer than 3 days.

For GI PCR Panel ordering questions please refer to the link at the top of the GI Wizard or Amion under (UCH) Clinical Laboratory.

For C. difficile Toxin PCR ordering questions please refer to the link at the top of the IP GI Wizard or Amion under (UCH) Infection Control.

Performed

Daily

Methodology

FilmArray® Multiplex PCR and culture.

Reported

Within 24 hours for Multiplex Film Array. Culture up to 72 hours.

Synonyms

  • UHGIPCR
  • GI PCR-11
  • GIPCRCULT
  • GI PCR with Supplemental Culture
  • Gastrointestinal Pathogens Panel by PCR
  • LAB6320 GI PCR 11 Target Panel - Outpatient only
  • LAB2005 GI PCR Panel Plus
  • IP GI WIZARD (FOR PATIENTS WITH DIARRHEA)
  • OP Diarrhea Wizard- Outpatient

Ordering Recommendations

Inpatient and ED ordering must occur through the GI Wizard. Restrictions apply to adults ≥18 years with GI PCR Panel only available during the first 2 days of admission.

EPIC Code

LAB6320 GI PCR-11 & GIPCRCULT
LAB2005 UHGIPCR & GIPCRCULT

UCHealth IP/OP orders must be placed through the Epic GI/Diarrhea Wizard.

Reference Interval

FilmArray® Multiplex PCR
ComponentFull GI Panel
Reference Range		11 Target
Reference Range
(will reflex to the full if any additional targets are detected)
CAMPYLOBACTERNot detectedNot detected
C. DIFFICILE A/B GENE

Not detected

*This is the PCR test for toxin A and toxin B genes of C. difficile.

Not detected

*This is the PCR test for toxin A and toxin B genes of C. difficile.

P. SHIGELLOIDESNot detectedNot detected
SALMONELLANot detectedNot detected
VIBRIONot detectedNot detected
VIBRIO CHOLERAENot detectedNot detected
Y. ENTEROCOLITICANot detectedNot detected
ENTEROAGGREGATIVE E. COLINot detectedNot detected
ENTEROPATHOGENIC E. COLINot detectedNot detected
ENTEROTOXIGENIC E. COLI LT/ST (ETEC)Not detectedNot detected
SHIGA-LIKE TOXIN-PRODUCING E. COLI STX1/STX2 (STEC)Not detectedNot detected

E. COLI O157

*n/a if STEC STX1/STX2 is not detected

Not detected
Not detected
SHIGELLA/ENTEROINVASIVE E.COLINot detectedNot detected
CRYPTOSPORIDIUMNot detectedNot detected
CYCLOSPORA CAYETANENSISNot detectedNot detected
ENTAMOEBA HISTOLYTICANot detectedNot detected
GIARDIA LAMBLIANot detectedNot detected
ADENOVIRUS F 40/41Not detectedNot detected
ASTROVIRUSNot detectedNot detected
NOROVIRUS GI/GIINot detectedNot detected
ROTAVIRUS ANot detectedNot detected
SAPOVIRUSNot detectedNot detected


Supplemental Culture for Aeromonas

Interpretive Data

Interpretation:
Not detected = Negative, the virus or bacteria indicated was not detected.
Detected = Positive, the virus or bacteria indicated was detected.

Methodology:
This test employs multiplex PCR amplification of viral and bacterial-specific conserved genetic targets. A positive result for this assay should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude involvement of the tested viruses or bacteria in a disease process. This test has been approved by the U.S. Food and Drug Administration for testing stool specimens in Cary-Blair media. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.

CPT Codes

87507
Collection

EPIC Code

LAB6320 GI PCR-11 & GIPCRCULT
LAB2005 UHGIPCR & GIPCRCULT

UCHealth IP/OP orders must be placed through the Epic GI/Diarrhea Wizard.

Performing Lab

UCHealth University of Colorado Hospital Clinical Laboratory - Molecular Diagnostics and Microbiology

Collect

Preferred: Stool in a Cary Blair Kit (dark green cap vial & white cap vial).
Acceptable: Stool in a Sterile Container. Stool in appropriately-filled Cary Blair alternative transport media with disclaimer (e.g., Cardinal Health™ C&S (orange cap vial), Cardinal Health™ Enteric Stool Transport Vial (lime green capped vial), or COPAN FecalSwab®).
Minimum volume: 0.5 mL of raw or preserved stool.

Patient Preparation

Specimens containing interfering substances (e.g., castor oil, bismuth, barium, etc.) may not have optimal yield for culture.

Specimen Preparation

Fresh stool added to Cary Blair Transport Kit (dark green & white caps) filled to the fill lines on the labels.

Outpatient Collection Note: Raw stool is only stable for 24 hours refrigerated. Stool received in appropriately-filled Cary Blair Transport Media is stable for 3 days.

Unacceptable Conditions

  • Formed stool.
  • Stool contaminated with urine.
  • Stool in diapers, tissue paper, or homemade containers.
  • Rectal swabs.
  • Frozen stool.
  • Stool not preserved in Cary Blair within 24 hours of collection.
  • Over- or under-filled Cary Blair submitted without at least 0.5 mL of stool in a non-additive, sterile container.
  • Samples from patients who have had a GI PCR <7 days ago.
  • Samples from hospitalized patients admitted >3 days.
  • Sample not properly identified, incorrect container, insufficient volume, or past stability.

Storage/Transport Temperature

Release and transport orders/labels for PCR ANDCulture together ("UHGIPCR"/"GI PCR-11" & “GIPCRCULT”).

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Stabilize and/or send to lab ASAP refrigerated.

Performed

Daily

Stability (from collection to initiation)

Room Temp/Refrigerated: 3 days in appropriately-filled Cary Blair (dark green vial); 24 hours in non-additive Sterile Container (white cap vial).
Frozen: Unacceptable.

Notes

Inpatient and ED ordering must occur through the IP GI Wizard. Restrictions apply to adults ≥18 years, with GI PCR Panel only available during the first 2 days of admission. C. diff Toxin EIA will only reflex from a GI Panel when C. diff A/B Gene is detected on patients admitted longer than 3 days.

For GI PCR Panel ordering questions please refer to the link at the top of the GI Wizard or Amion under (UCH) Clinical Laboratory.

For C. difficile Toxin PCR ordering questions please refer to the link at the top of the IP GI Wizard or Amion under (UCH) Infection Control.
Ordering

Performed

Daily

Methodology

FilmArray® Multiplex PCR and culture.

Reported

Within 24 hours for Multiplex Film Array. Culture up to 72 hours.

Synonyms

  • UHGIPCR
  • GI PCR-11
  • GIPCRCULT
  • GI PCR with Supplemental Culture
  • Gastrointestinal Pathogens Panel by PCR
  • LAB6320 GI PCR 11 Target Panel - Outpatient only
  • LAB2005 GI PCR Panel Plus
  • IP GI WIZARD (FOR PATIENTS WITH DIARRHEA)
  • OP Diarrhea Wizard- Outpatient

Ordering Recommendations

Inpatient and ED ordering must occur through the GI Wizard. Restrictions apply to adults ≥18 years with GI PCR Panel only available during the first 2 days of admission.

EPIC Code

LAB6320 GI PCR-11 & GIPCRCULT
LAB2005 UHGIPCR & GIPCRCULT

UCHealth IP/OP orders must be placed through the Epic GI/Diarrhea Wizard.

Result Interpretation

Reference Interval

FilmArray® Multiplex PCR
ComponentFull GI Panel
Reference Range		11 Target
Reference Range
(will reflex to the full if any additional targets are detected)
CAMPYLOBACTERNot detectedNot detected
C. DIFFICILE A/B GENE

Not detected

*This is the PCR test for toxin A and toxin B genes of C. difficile.

Not detected

*This is the PCR test for toxin A and toxin B genes of C. difficile.

P. SHIGELLOIDESNot detectedNot detected
SALMONELLANot detectedNot detected
VIBRIONot detectedNot detected
VIBRIO CHOLERAENot detectedNot detected
Y. ENTEROCOLITICANot detectedNot detected
ENTEROAGGREGATIVE E. COLINot detectedNot detected
ENTEROPATHOGENIC E. COLINot detectedNot detected
ENTEROTOXIGENIC E. COLI LT/ST (ETEC)Not detectedNot detected
SHIGA-LIKE TOXIN-PRODUCING E. COLI STX1/STX2 (STEC)Not detectedNot detected

E. COLI O157

*n/a if STEC STX1/STX2 is not detected

Not detected
Not detected
SHIGELLA/ENTEROINVASIVE E.COLINot detectedNot detected
CRYPTOSPORIDIUMNot detectedNot detected
CYCLOSPORA CAYETANENSISNot detectedNot detected
ENTAMOEBA HISTOLYTICANot detectedNot detected
GIARDIA LAMBLIANot detectedNot detected
ADENOVIRUS F 40/41Not detectedNot detected
ASTROVIRUSNot detectedNot detected
NOROVIRUS GI/GIINot detectedNot detected
ROTAVIRUS ANot detectedNot detected
SAPOVIRUSNot detectedNot detected


Supplemental Culture for Aeromonas

Interpretive Data

Interpretation:
Not detected = Negative, the virus or bacteria indicated was not detected.
Detected = Positive, the virus or bacteria indicated was detected.

Methodology:
This test employs multiplex PCR amplification of viral and bacterial-specific conserved genetic targets. A positive result for this assay should be coupled with clinical indicators for diagnosis.  A "Not detected" result for this assay does not exclude involvement of the tested viruses or bacteria in a disease process. This test has been approved by the U.S. Food and Drug Administration for testing stool specimens in Cary-Blair media. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendment of 1988 ("CLIA") as qualified to perform high complexity clinical testing.

Administrative

CPT Codes

87507

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