EPIC Code

LAB119

Two separate orders for
SPEP w/ Reflex IFE
SPEP Total Protein (needed for calculations)

Performing Lab

UCH Clinical Laboratory - Special Chemistry

Collect

Two labels will print.  Collect blood in two gold top tubes or two red tops with gel (SST):

  • SPEP w/reflex IFE = 1.0 mL serum (0.5 mL minimum)
  • SPEP Total Protein = 0.5 mL serum (0.3 mL minimum)

Pediatric Collection

Minimum volume:
2 gold top tubes, each with 3.0 mL whole blood
 

Unacceptable Conditions

Any plasma samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place 1.5 mL of serum in each of two aliquot containers and transport to lab at 2-8 degrees C. 

Performed

Monday through Friday

Stability (from collection to initiation)

Before centrifugation: 1 hour.

Serum aliquot: Ambient, 4 days; Refrigerated, 2 weeks; Frozen (-20 degrees C), 6 months.

Remarks


Serum Protein Electrophoresis (SPEP) and Total Protein are performed, and Immunofixation Electrophoresis (IFE) is also authorized to be performed when indicated.

Notes

Any Serum Protein Electrophoresis (SPEP) order that is placed within 3 days of a prior SPEP will be considered a duplicate order and will be cancelled. Due to the half-life of serum proteins, meaningful changes in SPEP results do not occur in less than 3 days.

Performed

Monday through Friday

Methodology

Serum protein electrophoresis. Immunofixation electrophoresis (IFE).

Reported

Within 3-7 days

Synonyms

  • SERUM PROTEIN ELECTROPHORESIS
  • LAB119
  • SPEP

EPIC Code

LAB119

Two separate orders for
SPEP w/ Reflex IFE
SPEP Total Protein (needed for calculations)

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units

PROTEIN TOTAL, SERUM/PLASMA

M/F 1M 150 6.4 8.9 g/dL
ALB FRACT M/F 0 150 3.5 4.8 g/dL
ALPHA 1 M/F 0 150 0.14 0.34 g/dL
ALPHA 2 M/F 0 150 0.5 1.0 g/dL
BETA M/F 0 150 0.8 1.5 g/dL
GAMMA M/F 0 150 0.4 1.4 g/dL

CPT Codes

84165 Protein Electrophoresis; and if indicated 86334 IFE serum.

Collection

EPIC Code

LAB119

Two separate orders for
SPEP w/ Reflex IFE
SPEP Total Protein (needed for calculations)

Performing Lab

UCH Clinical Laboratory - Special Chemistry

Collect

Two labels will print.  Collect blood in two gold top tubes or two red tops with gel (SST):

  • SPEP w/reflex IFE = 1.0 mL serum (0.5 mL minimum)
  • SPEP Total Protein = 0.5 mL serum (0.3 mL minimum)

Pediatric Collection

Minimum volume:
2 gold top tubes, each with 3.0 mL whole blood
 

Unacceptable Conditions

Any plasma samples; specimen not properly identified; insufficient sample volume.

Storage/Transport Temperature

Internal: Deliver to lab immediately at ambient temperature.
Offsite: Allow to clot 30 minutes, immediately centrifuge, place 1.5 mL of serum in each of two aliquot containers and transport to lab at 2-8 degrees C. 

Performed

Monday through Friday

Stability (from collection to initiation)

Before centrifugation: 1 hour.

Serum aliquot: Ambient, 4 days; Refrigerated, 2 weeks; Frozen (-20 degrees C), 6 months.

Remarks


Serum Protein Electrophoresis (SPEP) and Total Protein are performed, and Immunofixation Electrophoresis (IFE) is also authorized to be performed when indicated.

Notes

Any Serum Protein Electrophoresis (SPEP) order that is placed within 3 days of a prior SPEP will be considered a duplicate order and will be cancelled. Due to the half-life of serum proteins, meaningful changes in SPEP results do not occur in less than 3 days.

Ordering

Performed

Monday through Friday

Methodology

Serum protein electrophoresis. Immunofixation electrophoresis (IFE).

Reported

Within 3-7 days

Synonyms

  • SERUM PROTEIN ELECTROPHORESIS
  • LAB119
  • SPEP

EPIC Code

LAB119

Two separate orders for
SPEP w/ Reflex IFE
SPEP Total Protein (needed for calculations)

Result Interpretation

Reference Interval

Component Sex From Age To Age Normal Low Normal High Units

PROTEIN TOTAL, SERUM/PLASMA

M/F 1M 150 6.4 8.9 g/dL
ALB FRACT M/F 0 150 3.5 4.8 g/dL
ALPHA 1 M/F 0 150 0.14 0.34 g/dL
ALPHA 2 M/F 0 150 0.5 1.0 g/dL
BETA M/F 0 150 0.8 1.5 g/dL
GAMMA M/F 0 150 0.4 1.4 g/dL
Administrative

CPT Codes

84165 Protein Electrophoresis; and if indicated 86334 IFE serum.