Ordering Recommendations

This test is recommended for assessing a patient's immune status and provides serological evidence of prior exposure to HSV-1 and HSV-2.  It should NOT be used as a screening test for the general population.

Performed

Monday - Friday

Methodology

Chemiluminescence Immunoassay

Reported

Routine: 2 - 3 days

Synonyms

  • Herpes Simplex Virus Antibody, IgG
  • Herpes Simplex Virus 1 and 2 Antibody, IgG
  • Herpes Simplex Virus 1/2 Antibody, IgG
  • Herpes Simplex 1 and 2 Antibody, IgG
  • Herpes Simplex 1/2 Antibody, IgG
  • Herpes Antibody, IgG
  • Herpes Antibody Panel, IgG
  • Herpes Simplex Antibody Panel, IgG
  • Herpes Simplex Virus Antibody Panel, IgG
  • HSV Antibody, IgG
  • HSV Type 1/2 Antibody, IgG
  • HSV-1/2 Antibody, IgG
  • HSV1/2 Antibody, IgG
  • Herpes Simplex Virus Ab, IgG
  • Herpes Simplex Virus 1 and 2 Ab, IgG
  • Herpes Simplex Virus 1/2 Ab, IgG
  • Herpes Simplex 1 and 2 Ab, IgG
  • Herpes Simplex 1/2 Ab, IgG
  • Herpes Ab, IgG
  • HSV Type 1 and 2 Ab, IgG
  • HSV Ab, IgG
  • HSV-1/2 Ab, IgG
  • HSV1/2 Ab, IgG
  • Herpes Simplex Virus IgG Antibody
  • Herpes Simplex Virus 1/2 IgG Antibody
  • Herpes Simplex IgG Antibody
  • Herpes Simplex 1/2 IgG Antibody
  • Herpes IgG Antibody
  • HSV IgG Antibody
  • HSV-1/2 IgG Antibody
  • HSV1/2 IgG Antibody
  • HSV 1/2 IgG Ab
  • Herpes Antibodies
  • Herpes Simplex Antibodies
  • Herpes Serology
  • Herpes Simplex Serology
  • Herpes Simplex Immune Status
  • Microbiology Serology

Performing Lab

Microbiology

Add-on Eligibility

Yes

Tests Included

  1. Herpes Simplex 1 IgG Antibody
  2. Herpes Simplex 2 IgG Antibody

Specimen Type

Blood

Specimen Preparation

Blood specimens collected in Serum Separator Tubes (SSTs) must be centrifuged within 2 hours of the collection time for optimal sample integrity.

NOTE: Specimens collected in SSTs MUST sit for 30 minutes prior to centrifugation to allow for complete clot formation.

Specimen Volume

2.0 mL (Minimum: 1.0 mL)

Collection Container

Serum Separator Tube (SST, Gold Top Vacutainer)

Unacceptable Conditions

  1. More than one specimen within 7 days
  2. Lipemic or grossly hemolyzed specimens
  3. Specimens containing particulate matter or exhibiting obvious microbial contamination
  4. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than a Serum Separator Tube
  • Aliquot not made within 24 hours of collection
  • Specimens not refrigerated within 24 hours of collection
  • Specimens not frozen 7 days after collection
  1. Specimens containing less than 0.5 mL of serum once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions    
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 2 hours of collection
Laboratory/
Outpatient/
Off-Site
Room Temperature Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 7 days of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 2 hours
After separation from cells:
  • Room Temperature: 24 hours
  • Refrigerated: 7 days
  • Frozen at -20°C: Freeze serum aliquot for storage beyond 7 days

Laboratory Storage: Frozen
Laboratory Retention: 3 weeks

Collection Instructions

Labeling Instructions:
When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity. Ensure that the barcode is in the correct orientation.


Collection Instructions:
Follow the correct order of draw when collecting with additional orders and tube types:
     

Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Place the tube upright in a rack and allow the tube to sit for 30 minutes to ensure adequate clotting time
  3. Centrifuge within 2 hours of collection

Reference Interval

Negative

Interpretive Data

HSV-1 IgG Antibody Result Interpretation
Result Interpretation
Negative No detectable antibodies to HSV-1 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 - 6 weeks later.
Equivocal Equivocal samples are retested in duplicate by the LIAISON HSV-1 assay in order to confirm the equivocal result. A second sample should be collected and tested no less than 4 - 6 weeks later when the result is confirmed as equivocal.
Positive Indicates the presence of detectable IgG antibody to HSV-1.
 
HSV-2 IgG Antibody Result Interpretation
Result Interpretation
Negative No detectable antibodies to HSV-2 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 - 6 weeks later.
Equivocal Equivocal samples are retested in duplicate by the LIAISON HSV-2 assay in order to confirm the equivocal result. A second sample should be collected and tested no less than 4 - 6 weeks later when the result is confirmed as equivocal.
Positive Indicates the presence of detectable IgG antibody to HSV-2.
Ordering

Ordering Recommendations

This test is recommended for assessing a patient's immune status and provides serological evidence of prior exposure to HSV-1 and HSV-2.  It should NOT be used as a screening test for the general population.

Performed

Monday - Friday

Methodology

Chemiluminescence Immunoassay

Reported

Routine: 2 - 3 days

Synonyms

  • Herpes Simplex Virus Antibody, IgG
  • Herpes Simplex Virus 1 and 2 Antibody, IgG
  • Herpes Simplex Virus 1/2 Antibody, IgG
  • Herpes Simplex 1 and 2 Antibody, IgG
  • Herpes Simplex 1/2 Antibody, IgG
  • Herpes Antibody, IgG
  • Herpes Antibody Panel, IgG
  • Herpes Simplex Antibody Panel, IgG
  • Herpes Simplex Virus Antibody Panel, IgG
  • HSV Antibody, IgG
  • HSV Type 1/2 Antibody, IgG
  • HSV-1/2 Antibody, IgG
  • HSV1/2 Antibody, IgG
  • Herpes Simplex Virus Ab, IgG
  • Herpes Simplex Virus 1 and 2 Ab, IgG
  • Herpes Simplex Virus 1/2 Ab, IgG
  • Herpes Simplex 1 and 2 Ab, IgG
  • Herpes Simplex 1/2 Ab, IgG
  • Herpes Ab, IgG
  • HSV Type 1 and 2 Ab, IgG
  • HSV Ab, IgG
  • HSV-1/2 Ab, IgG
  • HSV1/2 Ab, IgG
  • Herpes Simplex Virus IgG Antibody
  • Herpes Simplex Virus 1/2 IgG Antibody
  • Herpes Simplex IgG Antibody
  • Herpes Simplex 1/2 IgG Antibody
  • Herpes IgG Antibody
  • HSV IgG Antibody
  • HSV-1/2 IgG Antibody
  • HSV1/2 IgG Antibody
  • HSV 1/2 IgG Ab
  • Herpes Antibodies
  • Herpes Simplex Antibodies
  • Herpes Serology
  • Herpes Simplex Serology
  • Herpes Simplex Immune Status
  • Microbiology Serology

Performing Lab

Microbiology

Add-on Eligibility

Yes
Collection

Tests Included

  1. Herpes Simplex 1 IgG Antibody
  2. Herpes Simplex 2 IgG Antibody

Specimen Type

Blood

Specimen Preparation

Blood specimens collected in Serum Separator Tubes (SSTs) must be centrifuged within 2 hours of the collection time for optimal sample integrity.

NOTE: Specimens collected in SSTs MUST sit for 30 minutes prior to centrifugation to allow for complete clot formation.

Specimen Volume

2.0 mL (Minimum: 1.0 mL)

Collection Container

Serum Separator Tube (SST, Gold Top Vacutainer)

Unacceptable Conditions

  1. More than one specimen within 7 days
  2. Lipemic or grossly hemolyzed specimens
  3. Specimens containing particulate matter or exhibiting obvious microbial contamination
  4. Specimens that have been improperly collected, stored, or transported
  • Specimens received in a collection tube other than a Serum Separator Tube
  • Aliquot not made within 24 hours of collection
  • Specimens not refrigerated within 24 hours of collection
  • Specimens not frozen 7 days after collection
  1. Specimens containing less than 0.5 mL of serum once separated from cells
  2. Unlabeled or mislabeled specimens

Storage/Transport Temperature

Transport Instructions    
Collection Location Transport Temperature Processing Required Timeframe
ED/Inpatient Room Temperature None Specimen must be received by the lab within 2 hours of collection
Laboratory/
Outpatient/
Off-Site
Room Temperature Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 24 hours of collection
Refrigerated (Ice Packs) Allow blood to clot for 30 minutes in a vertical position. Centrifuge to separate from cells within 2 hours of collection. Specimen must be received by the lab within 7 days of collection

Storage: Refrigerated

Stability (from collection to initiation)

Stability:
Prior to separation from cells:
  • Room Temperature: 2 hours
After separation from cells:
  • Room Temperature: 24 hours
  • Refrigerated: 7 days
  • Frozen at -20°C: Freeze serum aliquot for storage beyond 7 days

Laboratory Storage: Frozen
Laboratory Retention: 3 weeks

Collection Instructions

Labeling Instructions:
When labeling blood tubes, leave a small window visible for the lab to assess the fill volume and sample integrity. Ensure that the barcode is in the correct orientation.


Collection Instructions:
Follow the correct order of draw when collecting with additional orders and tube types:
     

Processing Instructions:
  1. Invert the collection tube according to the number of inversions in the chart above
  2. Place the tube upright in a rack and allow the tube to sit for 30 minutes to ensure adequate clotting time
  3. Centrifuge within 2 hours of collection
Result Interpretation

Reference Interval

Negative

Interpretive Data

HSV-1 IgG Antibody Result Interpretation
Result Interpretation
Negative No detectable antibodies to HSV-1 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 - 6 weeks later.
Equivocal Equivocal samples are retested in duplicate by the LIAISON HSV-1 assay in order to confirm the equivocal result. A second sample should be collected and tested no less than 4 - 6 weeks later when the result is confirmed as equivocal.
Positive Indicates the presence of detectable IgG antibody to HSV-1.
 
HSV-2 IgG Antibody Result Interpretation
Result Interpretation
Negative No detectable antibodies to HSV-2 were found. A negative result generally indicates that the patient has not been infected, but does not always rule out acute HSV infection. If clinical exposure to HSV is suspected despite a negative finding, a second sample should be collected and tested no less than 4 - 6 weeks later.
Equivocal Equivocal samples are retested in duplicate by the LIAISON HSV-2 assay in order to confirm the equivocal result. A second sample should be collected and tested no less than 4 - 6 weeks later when the result is confirmed as equivocal.
Positive Indicates the presence of detectable IgG antibody to HSV-2.