Blood in a QuantiFERON-TB (Standard) 4 In-Tube Collection Kit
1.0 mL, QuantiFERON-Nil Control Tube Grey cap with white ring
1.0 mL, QuantiFERON-TB1 Antigen Tube Green cap with white ring
1.0 mL, QuantiFERON-TB2Antigen Tube Yellow cap with white ring
1.0 mL, QuantiFERON-Mitogen Control Tube Purple cap with white ring
• Fill each tube with 1.0 mL of whole blood within the black marked line on each tube.
• Do not underfill or over fill the tubes.
• Immediately after filling the tubes, shake them ten (10) times with up and down motion enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on the tube walls.
• Over-energetic shaking may cause gel disruption and could lead to aberrant results
Specimen Required
4.0 mL of blood (in the QuantiFERON Gold In-tube collection kit)
Specimen Min Vol
1.0 mL per tube into each of the 4 QuantiFERON Gold collection kit tubes
Specimen Preparation
• Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood collection.
• Fill tubes correctly, underfilled or overfilled tubes will be rejected.
• Immediately after filling the tubes, shake them ten (10) times with up and down motion to ensure the entire inner surface of the tube is coated with blood, so solubilize antigens on the tube walls.
• After mixing transport tubes upright.
Storage/Transport Temperature
Refer to Specimen Stability criteria as shown below.
Stability (from collection to initiation)
after separation from cells:
Ambient
8 hours
Refrigerated
Unacceptable
Frozen
Unacceptable
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers, underfilled or overfilled tubes
Special Instructions
Collect specimens by 2:00 pm. Specimens MUST arrive in the lab by 5:00 pm. Specimen collected after 2:00 pm will have a delayed turnaround time.
CPT Codes
86480
Synonyms
QFT TB
Interferon Gamma Release assay for Tuberculosis
Latent Tuberculosis Infection (LTBI)
QuantiFERON Mitogen and NIL
Methodology
Chemiluminescent Immunoassay (CLIA)
Performing Location
Microbiology
Reported STAT
Not available
Reported Routine
1 to 3 days
Additional Information
Results Interpretation:
Negative M. tuberculosis infection NOT Likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) ≥ 0.5
Positive M. tuberculosis infection likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) ≥ Any
or
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) ≥ 0.35 and ≥25% of Nil
Mitogen minus Nil (IU/mL) Any
Indeterminate Likelihood of M. tuberculosis infection cannot be determined
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) < 0.5
or
Nil (IU/mL) > 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) Any
Test set up frequency
Monday to Friday
Reference Interval
Negative
Additional Information
Results Interpretation:
Negative M. tuberculosis infection NOT Likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) ≥ 0.5
Positive M. tuberculosis infection likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) ≥ Any
or
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) ≥ 0.35 and ≥25% of Nil
Mitogen minus Nil (IU/mL) Any
Indeterminate Likelihood of M. tuberculosis infection cannot be determined
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) < 0.5
or
Nil (IU/mL) > 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) Any
Blood in a QuantiFERON-TB (Standard) 4 In-Tube Collection Kit
1.0 mL, QuantiFERON-Nil Control Tube Grey cap with white ring
1.0 mL, QuantiFERON-TB1 Antigen Tube Green cap with white ring
1.0 mL, QuantiFERON-TB2Antigen Tube Yellow cap with white ring
1.0 mL, QuantiFERON-Mitogen Control Tube Purple cap with white ring
• Fill each tube with 1.0 mL of whole blood within the black marked line on each tube.
• Do not underfill or over fill the tubes.
• Immediately after filling the tubes, shake them ten (10) times with up and down motion enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on the tube walls.
• Over-energetic shaking may cause gel disruption and could lead to aberrant results
Specimen Required
4.0 mL of blood (in the QuantiFERON Gold In-tube collection kit)
Specimen Min Vol
1.0 mL per tube into each of the 4 QuantiFERON Gold collection kit tubes
Specimen Preparation
• Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood collection.
• Fill tubes correctly, underfilled or overfilled tubes will be rejected.
• Immediately after filling the tubes, shake them ten (10) times with up and down motion to ensure the entire inner surface of the tube is coated with blood, so solubilize antigens on the tube walls.
• After mixing transport tubes upright.
Storage/Transport Temperature
Refer to Specimen Stability criteria as shown below.
Stability (from collection to initiation)
after separation from cells:
Ambient
8 hours
Refrigerated
Unacceptable
Frozen
Unacceptable
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers, underfilled or overfilled tubes
Special Instructions
Collect specimens by 2:00 pm. Specimens MUST arrive in the lab by 5:00 pm. Specimen collected after 2:00 pm will have a delayed turnaround time.
Test Info
CPT Codes
86480
Synonyms
QFT TB
Interferon Gamma Release assay for Tuberculosis
Latent Tuberculosis Infection (LTBI)
QuantiFERON Mitogen and NIL
Methodology
Chemiluminescent Immunoassay (CLIA)
Performing Location
Microbiology
Reported STAT
Not available
Reported Routine
1 to 3 days
Additional Information
Results Interpretation:
Negative M. tuberculosis infection NOT Likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) ≥ 0.5
Positive M. tuberculosis infection likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) ≥ Any
or
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) ≥ 0.35 and ≥25% of Nil
Mitogen minus Nil (IU/mL) Any
Indeterminate Likelihood of M. tuberculosis infection cannot be determined
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) < 0.5
or
Nil (IU/mL) > 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) Any
Test set up frequency
Monday to Friday
Result Interpretation
Reference Interval
Negative
Additional Information
Results Interpretation:
Negative M. tuberculosis infection NOT Likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) ≥ 0.5
Positive M. tuberculosis infection likely
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) ≥ Any
or
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) ≥ 0.35 and ≥25% of Nil
Mitogen minus Nil (IU/mL) Any
Indeterminate Likelihood of M. tuberculosis infection cannot be determined
Nil (IU/mL) < = 8.0
TB1 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
TB2 Antigen minus Nil (IU/mL) < 0.35 or ≥0.35 and <25% of Nil
Mitogen minus Nil (IU/mL) < 0.5
or
Nil (IU/mL) > 8.0
TB1 Antigen minus Nil (IU/mL) Any
TB2 Antigen minus Nil (IU/mL) Any
Mitogen minus Nil (IU/mL) Any
