Preferred Bone Marrow aspirate. Acceptable: Leukemic blood, pleural/peritoneal fluid
Specimen Required
Freshly collected – 2.0 mL Bone Marrow Aspirate or 3.0 mL Peripheral Blood. Up to 50.0 mL of body fluids
Specimen Min Vol
1.0 mL of marrow, 2.0 mL blood, 50.0 mL of pleural/peritoneal fluid
Specimen Preparation
Use Sodium Heparin (Green top) preferably. EDTA (purple tube) is also acceptable. Invert several times to mix bone marrow/blood.
Collect body fluids in an aseptic container.
Ambient
Preferred. Transport to cytogenetics laboratory as soon as possible, store at room temperature up to 48 hours
Refrigerated
2-8 Celsius degree, If more than 48 hours after collection, and up to 10 days under proper refrigeration
Frozen
Unacceptable
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers/ Samples collected past ten (10) or more days/
Smear slides/Clotted specimen
Special Instructions
Order individual or multiple oncology FISH probes if standard FISH panels are not desired.
CPT Codes
88368
88369
88377
Methodology
Hybridization with commercial DNA probes of cultured cells or cells obtained by direct harvest. 200 interphase nuclei are scored. Two technologists analyze each probe set and all results are expressed as the percent abnormal nuclei.
Performing Location
Cytogenetics Laboratory
Reported STAT
1 - 3 days
Reported Routine
4 - 10 days
Additional Information
Email “CytogeneticsUCI@hs.uci.edu" with questions.
Time required for testing can vary depending on specimen type and probes ordered.
Please indicate the names of probes needed for testing.
Contact the Lab at CytogeneticsUCI@hs.uci.edu to add a probe to a current specimen.
This test was developed and its performance characteristics determined by UCI Cytogenetics Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Compliance Category: Laboratory Developed Test (LDT)
Test set up frequency
Monday - Friday
Reference Interval
As per specific FISH probe validation data
Additional Information
Email “CytogeneticsUCI@hs.uci.edu" with questions.
Time required for testing can vary depending on specimen type and probes ordered.
Please indicate the names of probes needed for testing.
Contact the Lab at CytogeneticsUCI@hs.uci.edu to add a probe to a current specimen.
This test was developed and its performance characteristics determined by UCI Cytogenetics Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Compliance Category: Laboratory Developed Test (LDT)
Collection
Collect
Preferred Bone Marrow aspirate. Acceptable: Leukemic blood, pleural/peritoneal fluid
Specimen Required
Freshly collected – 2.0 mL Bone Marrow Aspirate or 3.0 mL Peripheral Blood. Up to 50.0 mL of body fluids
Specimen Min Vol
1.0 mL of marrow, 2.0 mL blood, 50.0 mL of pleural/peritoneal fluid
Specimen Preparation
Use Sodium Heparin (Green top) preferably. EDTA (purple tube) is also acceptable. Invert several times to mix bone marrow/blood.
Collect body fluids in an aseptic container.
Ambient
Preferred. Transport to cytogenetics laboratory as soon as possible, store at room temperature up to 48 hours
Refrigerated
2-8 Celsius degree, If more than 48 hours after collection, and up to 10 days under proper refrigeration
Frozen
Unacceptable
Unacceptable Conditions
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers/ Samples collected past ten (10) or more days/
Smear slides/Clotted specimen
Special Instructions
Order individual or multiple oncology FISH probes if standard FISH panels are not desired.
Test Info
CPT Codes
88368
88369
88377
Methodology
Hybridization with commercial DNA probes of cultured cells or cells obtained by direct harvest. 200 interphase nuclei are scored. Two technologists analyze each probe set and all results are expressed as the percent abnormal nuclei.
Performing Location
Cytogenetics Laboratory
Reported STAT
1 - 3 days
Reported Routine
4 - 10 days
Additional Information
Email “CytogeneticsUCI@hs.uci.edu" with questions.
Time required for testing can vary depending on specimen type and probes ordered.
Please indicate the names of probes needed for testing.
Contact the Lab at CytogeneticsUCI@hs.uci.edu to add a probe to a current specimen.
This test was developed and its performance characteristics determined by UCI Cytogenetics Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Compliance Category: Laboratory Developed Test (LDT)
Test set up frequency
Monday - Friday
Result Interpretation
Reference Interval
As per specific FISH probe validation data
Additional Information
Email “CytogeneticsUCI@hs.uci.edu" with questions.
Time required for testing can vary depending on specimen type and probes ordered.
Please indicate the names of probes needed for testing.
Contact the Lab at CytogeneticsUCI@hs.uci.edu to add a probe to a current specimen.
This test was developed and its performance characteristics determined by UCI Cytogenetics Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Compliance Category: Laboratory Developed Test (LDT)
