Collection Containers

Light Blue 2.7 mL
Light Blue 1.8 mL (Blue Stopper/Clear Cap)

Collection Instructions

Completely fill Light Blue tube to the line to attain the correct ratio of blood to citrate (9:1).
Invert the tube gently three or four times immediately after venipuncture to ensure proper mixing of blood and anticoagulant.


Preferred Specimen Volume

Plasma from Light Blue 2.7 mL   

Minimum Specimen Volume

Plasma from Light Blue 1.8 mL   

Specimen Handling

Submit to laboratory within 24 hours of collection.

If shipping is delayed >24 hours, plasma must be frozen.
Coagulation Double Spin Procedure: Use designated coagulation centrifuge and settings. Centrifuge specimen to obtain platelet poor plasma. After centrifugation, remove plasma and transfer to polypropylene aliquot tube. Centrifuge aliquotted specimen again. After centrifugation, remove plasma for the second time and transfer to a new polypropylene aliquot tube. Do not pool plasma from individual sodium citrate or aliquot tubes.

Specimen Stability

Room Temperature: 24 hours (centrifuged or uncentrifuged) capped
Refrigerated: Unacceptable
Frozen: 2 weeks (-20 degrees), 6 months (-70 degrees)

Shipping Temperature

Room Temperature

Unacceptable Conditions

Clotted Specimens
Specimens collected in the wrong anticoagulant: (3.2% sodium citrate is the only acceptable anticoagulant)
Gross hemolysis
Under-filled or overfilled tubes by 10%
Non-polypropylene aliquot tubes
Samples kept at room temperature longer than 24 hours after collection
Aliquot tubes with small plasma volumes as it may cause pH changes in the plasma leading to inactivation of specific components of the coagulation system.
Specimens received refrigerated.

Remarks

Freeze aliquot if testing is delayed longer than 24 hours.
Ensure specimen is frozen solid prior to shipment.
Specimens must remain frozen during storage and shipment.

Patient Preparation

No additional special requirements.

Ordering Recommendations

Initial test for suspected bleeding disorder.
Recommended to monitor individuals on warfarin therapy.

Methodology

LED optical detection

Test Components

Protime
INR

Performed

Daily

Turnaround Time (TAT) Reported

STAT: 30 Min
Routine: 2 Hours

Performing Laboratories

Boise Medical Center
Elmore Medical Center
Fruitland Medical Center
Jerome Medical Center
Magic Valley Medical Center
McCall Medical Center
Meridian Medical Center
Nampa Medical Center
Wood River Medical Center

Synonyms

  • PT INR Prothrombin Time

Interpretive Data

INR:
Recommended theraputic ranges:  
  • Venous Thromboembolism: 2.0-3.0  
  • Mechanical heart valves-Arterial embolism: 2.5-3.5
Pediatric Reference Ranges include the following statement:
Reference intervals provided for pediatric patients have not been internally validated, these values are referenced from current industry literature. Results should only be evaluated in conjunction with the entire clinical presentation, interpret with caution.

Reference Ranges

Patient Age Prothrombin Time Reference Range (Seconds)
Newborn 9.2 - 14.3
15 days - 4 weeks 9.5 - 12.6
1 - 5 months 9.7 - 12.8
6 - 11 months 9.8 - 13.0
1 - 5 years 9.9 - 13.4
6 - 10 years 10.0 - 14.6
11 - 17 years 10.0 - 14.1
Adult (18+ years) 10.1 - 13.1

Normal Reference Range for INR Ratio: 0.9 - 1.1
Prothrombin Time Geomean: 11.6 Seconds (Lot N0925650)
Prothrombin Time Geomean: 11.2 Seconds (Lot N0835088)

CPT Codes

85610
Collection

Collection Containers

Light Blue 2.7 mL
Light Blue 1.8 mL (Blue Stopper/Clear Cap)

Collection Instructions

Completely fill Light Blue tube to the line to attain the correct ratio of blood to citrate (9:1).
Invert the tube gently three or four times immediately after venipuncture to ensure proper mixing of blood and anticoagulant.


Preferred Specimen Volume

Plasma from Light Blue 2.7 mL   

Minimum Specimen Volume

Plasma from Light Blue 1.8 mL   

Specimen Handling

Submit to laboratory within 24 hours of collection.

If shipping is delayed >24 hours, plasma must be frozen.
Coagulation Double Spin Procedure: Use designated coagulation centrifuge and settings. Centrifuge specimen to obtain platelet poor plasma. After centrifugation, remove plasma and transfer to polypropylene aliquot tube. Centrifuge aliquotted specimen again. After centrifugation, remove plasma for the second time and transfer to a new polypropylene aliquot tube. Do not pool plasma from individual sodium citrate or aliquot tubes.

Specimen Stability

Room Temperature: 24 hours (centrifuged or uncentrifuged) capped
Refrigerated: Unacceptable
Frozen: 2 weeks (-20 degrees), 6 months (-70 degrees)

Shipping Temperature

Room Temperature

Unacceptable Conditions

Clotted Specimens
Specimens collected in the wrong anticoagulant: (3.2% sodium citrate is the only acceptable anticoagulant)
Gross hemolysis
Under-filled or overfilled tubes by 10%
Non-polypropylene aliquot tubes
Samples kept at room temperature longer than 24 hours after collection
Aliquot tubes with small plasma volumes as it may cause pH changes in the plasma leading to inactivation of specific components of the coagulation system.
Specimens received refrigerated.

Remarks

Freeze aliquot if testing is delayed longer than 24 hours.
Ensure specimen is frozen solid prior to shipment.
Specimens must remain frozen during storage and shipment.
Ordering

Patient Preparation

No additional special requirements.

Ordering Recommendations

Initial test for suspected bleeding disorder.
Recommended to monitor individuals on warfarin therapy.

Methodology

LED optical detection

Test Components

Protime
INR

Performed

Daily

Turnaround Time (TAT) Reported

STAT: 30 Min
Routine: 2 Hours

Performing Laboratories

Boise Medical Center
Elmore Medical Center
Fruitland Medical Center
Jerome Medical Center
Magic Valley Medical Center
McCall Medical Center
Meridian Medical Center
Nampa Medical Center
Wood River Medical Center

Synonyms

  • PT INR Prothrombin Time
Result Interpretation

Interpretive Data

INR:
Recommended theraputic ranges:  
  • Venous Thromboembolism: 2.0-3.0  
  • Mechanical heart valves-Arterial embolism: 2.5-3.5
Pediatric Reference Ranges include the following statement:
Reference intervals provided for pediatric patients have not been internally validated, these values are referenced from current industry literature. Results should only be evaluated in conjunction with the entire clinical presentation, interpret with caution.

Reference Ranges

Patient Age Prothrombin Time Reference Range (Seconds)
Newborn 9.2 - 14.3
15 days - 4 weeks 9.5 - 12.6
1 - 5 months 9.7 - 12.8
6 - 11 months 9.8 - 13.0
1 - 5 years 9.9 - 13.4
6 - 10 years 10.0 - 14.6
11 - 17 years 10.0 - 14.1
Adult (18+ years) 10.1 - 13.1

Normal Reference Range for INR Ratio: 0.9 - 1.1
Prothrombin Time Geomean: 11.6 Seconds (Lot N0925650)
Prothrombin Time Geomean: 11.2 Seconds (Lot N0835088)
Administrative

CPT Codes

85610

Order of Draw

Microtainer (NICU/Peds) Order of Draw
Order of Draw (BD/ Greiner)

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