Ordering Recommendations

Order MENCP for CSF samples.

Collect

Plasma, serum, trach asp, trach wash, vitreous fluid, aqueous fluid, tissue, rectal/anal, perineum, amniotic fluid
Skin swabs, eye swabs, genital swabs, bronchoaveolar lavage.
Universal Transport Medium (UTM) for swabs; Sterile container for fluids, and tissue; Syringe for aqueous and vitreous fluid; EDTA, or red top, for blood.
For newborns/infants (< 18 months old), collect a minimum of 2 mL lavender (EDTA) tubes or 4 lavender (EDTA) microtainers (pediatric bullet tubes)

Specimen Preparation

Separate plasma or serum from cells.

Storage/Transport Temperature

Transport specimen to laboratory at room temperature

Unacceptable Conditions

Heparinized specimens.
Specimens received more than 96 hours after collection.
Avoid using gel separator tubes.  Samples collected in gel separator tubes have increased rate of PCR inhibition.

Remarks

Specimen source required

Stability (from collection to initiation)

Tissue: Frozen: 3 months
All Others: Refrigerated: 72 hours; Frozen: 3 months

Collection device

UNIVERSAL TRANSPORT MEDIA

Minimum Requirements

2.0 mL of specimen or swab in viral transport media (VTM) or universal transport media (UTM)
0.2 mL eye fluid
2.0 mL amniotic fluid
0.5 mL plasma or serum
1 mL BAL
 

Alternate Specimen Requirements

Please contact the Client Services at 585-758-0510 for requests.

Lab Section

Microbiology (Virology)

Methodology

PCR

Performed

Daily
Amniotic Fluid: Sun-Sat

Reported

1-3 days
Amniotic fluid: 2-4 days

Synonyms

  • HSV PCR
  • HSV Types 1&2 DNA PCR

Reference Lab Number

8500

Performing Laboratory Website (click below)

UR Medicine Labs for skin lesions or vesicles, BAL, BB, B Wash, or genital swabs, skin swabs

https://www.aruplab.com/ for amniotic fluid

www.eurofins-viracor.com for all other sources

Notes

 HSV-1 and HSV-2 are well known etiologic agents of gingivostomatitis, conjunctivitis, keratitis, herpetic whitlow, and genital infections as well as encephalitis, aseptic meningitis, atypical pneumonias and a wide variety of other clinical conditions.

Reference Interval

See report

CPT Codes

URMC performs HSV PCR with CPT 87529

LOINC Mapping

HSV Type 1 DNA PCR: 16130-7
HSV Type 2 DNA PCR: 16131-5

Order Type (Individual or Group)

I

Result Test ID

HSVA
HSVB
 

Reportable?

Y

Result Test Name

HSV TYPE 1 DNA PCR
HSV TYPE 2 DNA PCR

Result Type (Individual or Group)

I

Type (Alpha or Numeric)

A

Default Result

See Micro

Text

BLOOD: LAV OR SST or RED SWAB: UNIVERSAL TRANSPORT MEDIA ALL OTHER SPECIMENS: STERILE CONTAINER

Reflex Test ID

 Reflex Test ID  Reportable  Result Test Name Result Type Type (Alpha or Numeric)
HSVA Y HSV TYPE 1 DNA PCR I A
HSVB Y HSV TYPE 2 DNA PCR I A

Micro Source Information

 
SOURCE ID         SOURCE NAME SITES REFLEX TESTS
AMNIO AMNIOTIC FLUID   HSVA
HSVB
AQFL AQUEOUS FLUID   HSVA
HSVB
BA BRONCHIAL ASPIRATE   HSVA
HSVB
BAL BRONCHIAL ALVEOLAR LAVAGE RIGHT UPPER LOBE
RIGHT MIDDLE LOBE
RIGHT LOWER LOBE
LEFT UPPER LOBE
LEFT MIDDLE LOBE		 HSVA
HSVB
BLIS BLISTER   HSVA
HSVB
BLOOD BLOOD   HSVA
HSVB
BUTCK BUTTOCK   HSVA
HSVB
BW BRONCHIAL WASH   HSVA
HSVB
CONJ CONJUNCTIVA LEFT 
RIGHT		 HSVA
HSVB
CORNS CORNEAL SCRAPING  LEFT
RIGHT		 HSVA
HSVB
EYE EYE LEFT
RIGHT		 HSVA
HSVB
LAL LABIAL LESION   HSVA
HSVB
LES LESION   HSVA
HSVB
LIP LIP   HSVA
HSVB
PELV PELVIS   HSVA
HSVB
PENIS PENIS   HSVA
HSVB
PERIN PERINEUM   HSVA
HSVB
PLA PLASMA   HSVA
HSVB
PUST PUSTULE   HSVA
HSVB
RASH RASH   HSVA
HSVB
RECTA RECTAL/ANAL   HSVA
HSVB
SCRO SCROTUM   HSVA
HSVB
SERUM SERUM   HSVA
HSVB
SKIN SKIN   HSVA
HSVB
TA TRACHEAL ASPIRATE   HSVA
HSVB
TISSU TISSUE   HSVA
HSVB
TONG TONGUE   HSVA
HSVB
ULCER ULCER   HSVA
HSVB
VAG VAGINA   HSVA
HSVB
VES VESICLE   HSVA
HSVB
VITF VITREOUS FLUID   HSVA
HSVB
VULVA VULVA   HSVA
HSVB

CPT Codes

URMC performs HSV PCR with CPT 87529

LOINC Mapping

HSV Type 1 DNA PCR: 16130-7
HSV Type 2 DNA PCR: 16131-5

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements

Ordering Recommendations

Order MENCP for CSF samples.

Collect

Plasma, serum, trach asp, trach wash, vitreous fluid, aqueous fluid, tissue, rectal/anal, perineum, amniotic fluid
Skin swabs, eye swabs, genital swabs, bronchoaveolar lavage.
Universal Transport Medium (UTM) for swabs; Sterile container for fluids, and tissue; Syringe for aqueous and vitreous fluid; EDTA, or red top, for blood.
For newborns/infants (< 18 months old), collect a minimum of 2 mL lavender (EDTA) tubes or 4 lavender (EDTA) microtainers (pediatric bullet tubes)

Specimen Preparation

Separate plasma or serum from cells.

Storage/Transport Temperature

Transport specimen to laboratory at room temperature

Unacceptable Conditions

Heparinized specimens.
Specimens received more than 96 hours after collection.
Avoid using gel separator tubes.  Samples collected in gel separator tubes have increased rate of PCR inhibition.

Remarks

Specimen source required

Stability (from collection to initiation)

Tissue: Frozen: 3 months
All Others: Refrigerated: 72 hours; Frozen: 3 months

Collection device

UNIVERSAL TRANSPORT MEDIA

Minimum Requirements

2.0 mL of specimen or swab in viral transport media (VTM) or universal transport media (UTM)
0.2 mL eye fluid
2.0 mL amniotic fluid
0.5 mL plasma or serum
1 mL BAL
 

Alternate Specimen Requirements

Please contact the Client Services at 585-758-0510 for requests.
Testing

Lab Section

Microbiology (Virology)

Methodology

PCR

Performed

Daily
Amniotic Fluid: Sun-Sat

Reported

1-3 days
Amniotic fluid: 2-4 days

Synonyms

  • HSV PCR
  • HSV Types 1&2 DNA PCR

Reference Lab Number

8500

Performing Laboratory Website (click below)

UR Medicine Labs for skin lesions or vesicles, BAL, BB, B Wash, or genital swabs, skin swabs

https://www.aruplab.com/ for amniotic fluid

www.eurofins-viracor.com for all other sources

Notes

 HSV-1 and HSV-2 are well known etiologic agents of gingivostomatitis, conjunctivitis, keratitis, herpetic whitlow, and genital infections as well as encephalitis, aseptic meningitis, atypical pneumonias and a wide variety of other clinical conditions.
Result Interpretation

Reference Interval

See report
Coding

CPT Codes

URMC performs HSV PCR with CPT 87529

LOINC Mapping

HSV Type 1 DNA PCR: 16130-7
HSV Type 2 DNA PCR: 16131-5
URM Labs Internal
Test Build

Order Type (Individual or Group)

I

Result Test ID

HSVA
HSVB
 

Reportable?

Y

Result Test Name

HSV TYPE 1 DNA PCR
HSV TYPE 2 DNA PCR

Result Type (Individual or Group)

I

Type (Alpha or Numeric)

A

Default Result

See Micro

Text

BLOOD: LAV OR SST or RED SWAB: UNIVERSAL TRANSPORT MEDIA ALL OTHER SPECIMENS: STERILE CONTAINER

Reflex Test ID

 Reflex Test ID  Reportable  Result Test Name Result Type Type (Alpha or Numeric)
HSVA Y HSV TYPE 1 DNA PCR I A
HSVB Y HSV TYPE 2 DNA PCR I A

Micro Source Information

 
SOURCE ID         SOURCE NAME SITES REFLEX TESTS
AMNIO AMNIOTIC FLUID   HSVA
HSVB
AQFL AQUEOUS FLUID   HSVA
HSVB
BA BRONCHIAL ASPIRATE   HSVA
HSVB
BAL BRONCHIAL ALVEOLAR LAVAGE RIGHT UPPER LOBE
RIGHT MIDDLE LOBE
RIGHT LOWER LOBE
LEFT UPPER LOBE
LEFT MIDDLE LOBE		 HSVA
HSVB
BLIS BLISTER   HSVA
HSVB
BLOOD BLOOD   HSVA
HSVB
BUTCK BUTTOCK   HSVA
HSVB
BW BRONCHIAL WASH   HSVA
HSVB
CONJ CONJUNCTIVA LEFT 
RIGHT		 HSVA
HSVB
CORNS CORNEAL SCRAPING  LEFT
RIGHT		 HSVA
HSVB
EYE EYE LEFT
RIGHT		 HSVA
HSVB
LAL LABIAL LESION   HSVA
HSVB
LES LESION   HSVA
HSVB
LIP LIP   HSVA
HSVB
PELV PELVIS   HSVA
HSVB
PENIS PENIS   HSVA
HSVB
PERIN PERINEUM   HSVA
HSVB
PLA PLASMA   HSVA
HSVB
PUST PUSTULE   HSVA
HSVB
RASH RASH   HSVA
HSVB
RECTA RECTAL/ANAL   HSVA
HSVB
SCRO SCROTUM   HSVA
HSVB
SERUM SERUM   HSVA
HSVB
SKIN SKIN   HSVA
HSVB
TA TRACHEAL ASPIRATE   HSVA
HSVB
TISSU TISSUE   HSVA
HSVB
TONG TONGUE   HSVA
HSVB
ULCER ULCER   HSVA
HSVB
VAG VAGINA   HSVA
HSVB
VES VESICLE   HSVA
HSVB
VITF VITREOUS FLUID   HSVA
HSVB
VULVA VULVA   HSVA
HSVB

CPT Codes

URMC performs HSV PCR with CPT 87529

LOINC Mapping

HSV Type 1 DNA PCR: 16130-7
HSV Type 2 DNA PCR: 16131-5

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu

Important Information

Lab Section
Microbiology (Virology)

ARUP Consult®

Disease Topics

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