Isolator tubes or Yellow Top Vacutainer tubes with SPS are used for the optimal recovery of Mycobacteria in blood. Order Test Code: BIAFB
Collect
Whole Blood: Collect one 10 mL Wampole Isolator tube. If Isolator tube is not available, collect one 8 mL Yellow Top Vacutainer Tube with SPS as anticoagulant. Regardless of tube type, fill with maximum draw if possible: pediatric tubes are not available.
For SMH Inpatient Collections: Obtain collection tubes from hospital stores
For Affiliate Hospitals and Outpatient Collections: Refer to supply ordering website to obtain collection tubes
Storage/Transport Temperature
Submit all in sealed biohazard bags for specimen transport.
Transport to microbiology laboratory at room temperature as soon as possilbe after collection.
Unacceptable Conditions
Leaking tubes
Specimens in tubes with anticoagulant other than SPS
Refrigerated specimens
Remarks
CRITICAL AMBIENT. Submit within 16 hours after collection
Stability (from collection to initiation)
Ambient: 16 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Do NOT refrigerate or freeze
Test Barcode Number
33488
Lab Section
Microbiology (Mycobacteriology)
Methodology
Lysis Concentration culture with standard procedures for identification of Mycobacteria
Performed
7 days per week
Reported
Negative cultures are finalized after 5 weeks of incubation.
Positive cultures are finalized after organism identification has been completed
AFB Identification and drug susceptbility tests are billed separately from culture
Reference Interval
No growth
CPT Codes
87116 for culture and 87015 for centrifugation
Order Type (Individual or Group)
I
Result Test ID
BIAFB
Reportable?
Y
Result Test Name
AFB BLOOD ISOLATOR CULTURE
Result Type (Individual or Group)
I
Type (Alpha or Numeric)
A
Default Result
See Micro
Reflex Test ID
Reflex Test ID
Reportable
Result Test Name
Result type
Type(alpha or numeric)
AFBRL
Y
AFB SUSCEPTIBILITIES
I
A
TBNYS
Y
TB SUSCEPTIBILITIES
I
A
Micro Source Information
Source ID
Source Name
Sites
REFLEX TESTS
BLOOD
BLOOD
CPT Codes
87116 for culture and 87015 for centrifugation
Pricing
Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements
Ordering Recommendations
Isolator tubes or Yellow Top Vacutainer tubes with SPS are used for the optimal recovery of Mycobacteria in blood. Order Test Code: BIAFB
Collect
Whole Blood: Collect one 10 mL Wampole Isolator tube. If Isolator tube is not available, collect one 8 mL Yellow Top Vacutainer Tube with SPS as anticoagulant. Regardless of tube type, fill with maximum draw if possible: pediatric tubes are not available.
For SMH Inpatient Collections: Obtain collection tubes from hospital stores
For Affiliate Hospitals and Outpatient Collections: Refer to supply ordering website to obtain collection tubes
Storage/Transport Temperature
Submit all in sealed biohazard bags for specimen transport.
Transport to microbiology laboratory at room temperature as soon as possilbe after collection.
Unacceptable Conditions
Leaking tubes
Specimens in tubes with anticoagulant other than SPS
Refrigerated specimens
Remarks
CRITICAL AMBIENT. Submit within 16 hours after collection
Stability (from collection to initiation)
Ambient: 16 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Do NOT refrigerate or freeze
Test Barcode Number
33488
Testing
Lab Section
Microbiology (Mycobacteriology)
Methodology
Lysis Concentration culture with standard procedures for identification of Mycobacteria
Performed
7 days per week
Reported
Negative cultures are finalized after 5 weeks of incubation.
Positive cultures are finalized after organism identification has been completed
