Collect

Peripheral Blood (Whole Blood): (5-8mL) in green top sodium heparin tube
Amniotic fluid: 15-30mL in sterile container
CVS: 15-25mg villi in sterile Hanks sol. (available from lab upon request)
Bone Marrow: 2-4mL in green top sodium heparin tube
Tumor/Tissue: Tissue in sterile container with sterile Hanks sol. (available from lab upon request) or sterile saline
POC: 1 - 2cm2 piece of tissue in sterile saline or transport media (available from lab upon request). 
FFPE: 4 micron section on positively charged microscope slides if indicated. 

Specimen Preparation

Label tube with patient name, second identifier, date of collection and the contents of the tube

Storage/Transport Temperature

Transport specimens at room temperature as soon as possible to the cytogenetics laboratory.  If there is a delay, refrigerate (2-8°C) specimen.  
FFPE slides should be shipped and stored at room temperature

Remarks

Specimens should be accompanied by a completed requisition that should include patient information, diagnosis or ICD-10 code, physician signature, insurance information, test requested (include specific DNA probe) and signed patient consent. Signed patient consent is not necessary for Oncology specimens. To obtain consent form/requisition for constitutional cases click here: Cytogenetics Requisition/Consent Form

Click here for descriptions of FISH testing that require a consent form.

Test Barcode Number

16807

Lab Section

Cytogenetics

Methodology

Fluorescence In-Situ Hybridization (FISH)

Performed

Mon - Fri, day shift. STAT FISH testing is available upon request.

Reported

7-14 days

Performing Laboratory Website (click below)

Notes

Components: FISH cells, FISH diagnosis

Reference Interval

See report

CPT Codes

88271 (quantity will depend on number of probes)
88273 if analyzing 10 - 30 metaphases
88274 if analyzing 25 - 99 interphase cells; OR 88275 if analyzing 100 - 300 interphase cells.
Oncology specimens for FISH analysis may have additional probes and interphase cells added.  Therefore additional CPT codes would apply

LOINC Mapping

FISH - CVS: 55193-7
FISH-AF: 55192-9
FISH-Oncology: 57802-1
FISH-138+ Cells: 57802-1

Order Type (Individual or Group)

G

Group Test Information

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
STCHR Y SPECIMEN TYPE I A
CASE# Y PATH CASE # I A
IDCHR Y INDICATION I A
PTYPE Y PROBE TYPE I A
PROBE Y # OF PROBES I N
FISHC Y FISH CELLS I N
FISHD Y FISH DIAGNOSIS I A
INTFH Y INTERP,FISH I A
REVFH Y REVIEWED BY: I A

Reflex Test ID

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
CTCST Y PATIENT CONSENT I A
RRFIS Y FISH REVIEW I A

CDATE  only relfexed if CTCST is resulted as NO.
Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
CDATE  Y CONSENT FORM/CANCEL DATE Y A

 

CPT Codes

88271 (quantity will depend on number of probes)
88273 if analyzing 10 - 30 metaphases
88274 if analyzing 25 - 99 interphase cells; OR 88275 if analyzing 100 - 300 interphase cells.
Oncology specimens for FISH analysis may have additional probes and interphase cells added.  Therefore additional CPT codes would apply

LOINC Mapping

FISH - CVS: 55193-7
FISH-AF: 55192-9
FISH-Oncology: 57802-1
FISH-138+ Cells: 57802-1

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements

Collect

Peripheral Blood (Whole Blood): (5-8mL) in green top sodium heparin tube
Amniotic fluid: 15-30mL in sterile container
CVS: 15-25mg villi in sterile Hanks sol. (available from lab upon request)
Bone Marrow: 2-4mL in green top sodium heparin tube
Tumor/Tissue: Tissue in sterile container with sterile Hanks sol. (available from lab upon request) or sterile saline
POC: 1 - 2cm2 piece of tissue in sterile saline or transport media (available from lab upon request). 
FFPE: 4 micron section on positively charged microscope slides if indicated. 

Specimen Preparation

Label tube with patient name, second identifier, date of collection and the contents of the tube

Storage/Transport Temperature

Transport specimens at room temperature as soon as possible to the cytogenetics laboratory.  If there is a delay, refrigerate (2-8°C) specimen.  
FFPE slides should be shipped and stored at room temperature

Remarks

Specimens should be accompanied by a completed requisition that should include patient information, diagnosis or ICD-10 code, physician signature, insurance information, test requested (include specific DNA probe) and signed patient consent. Signed patient consent is not necessary for Oncology specimens. To obtain consent form/requisition for constitutional cases click here: Cytogenetics Requisition/Consent Form

Click here for descriptions of FISH testing that require a consent form.

Test Barcode Number

16807
Testing

Lab Section

Cytogenetics

Methodology

Fluorescence In-Situ Hybridization (FISH)

Performed

Mon - Fri, day shift. STAT FISH testing is available upon request.

Reported

7-14 days

Performing Laboratory Website (click below)

Notes

Components: FISH cells, FISH diagnosis
Result Interpretation

Reference Interval

See report
Coding

CPT Codes

88271 (quantity will depend on number of probes)
88273 if analyzing 10 - 30 metaphases
88274 if analyzing 25 - 99 interphase cells; OR 88275 if analyzing 100 - 300 interphase cells.
Oncology specimens for FISH analysis may have additional probes and interphase cells added.  Therefore additional CPT codes would apply

LOINC Mapping

FISH - CVS: 55193-7
FISH-AF: 55192-9
FISH-Oncology: 57802-1
FISH-138+ Cells: 57802-1
URM Labs Internal
Test Build

Order Type (Individual or Group)

G

Group Test Information

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
STCHR Y SPECIMEN TYPE I A
CASE# Y PATH CASE # I A
IDCHR Y INDICATION I A
PTYPE Y PROBE TYPE I A
PROBE Y # OF PROBES I N
FISHC Y FISH CELLS I N
FISHD Y FISH DIAGNOSIS I A
INTFH Y INTERP,FISH I A
REVFH Y REVIEWED BY: I A

Reflex Test ID

Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
CTCST Y PATIENT CONSENT I A
RRFIS Y FISH REVIEW I A

CDATE  only relfexed if CTCST is resulted as NO.
Result Test ID Reportable Result Test Name Result Type Type (Alpha or Numeric)
CDATE  Y CONSENT FORM/CANCEL DATE Y A

 

CPT Codes

88271 (quantity will depend on number of probes)
88273 if analyzing 10 - 30 metaphases
88274 if analyzing 25 - 99 interphase cells; OR 88275 if analyzing 100 - 300 interphase cells.
Oncology specimens for FISH analysis may have additional probes and interphase cells added.  Therefore additional CPT codes would apply

LOINC Mapping

FISH - CVS: 55193-7
FISH-AF: 55192-9
FISH-Oncology: 57802-1
FISH-138+ Cells: 57802-1

Pricing

Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu