Heme samples : one 5 mL blood in EDTA (lav top), or one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells)
Fresh tissue: min 0.1mL
Frozen tissue: min 100mg or 0.5cc / 0.5 cm3
Body Fluids: >200K cells
Storage/Transport Temperature
20-25°C for several hours acceptable
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.
Frozen samples should be sent on dry ice.
A positive result indicates the presence of FLT3 internal tandem duplication (ITD) or a tyrosine kinase domain (TKD) point mutation in codons 835 or 836. The limit of detection for this assay is 1 in 20 cells. Results of this test must always be interpreted in the context of clinicopathologic and relevant data, and should not be used alone for a diagnosis of malignancy. Methodology: Patient DNA is isolated, purified and subjected to PCR amplification using specific oligonucleotide primers directed at exons 14, 15, and 20 of the FLT3 gene. Each assay includes a positive control reaction using DNA from a specimen or cell line known to contain a FLT3-ITD mutation or exon 20 point mutation and a negative control reaction using DNA from a specimen or cell line containing the wild-type FLT3 sequence. Amplicons for exon 20 are digested with EcoR V. PCR products are separated by electrophoresis. The limit of detection for this assay is 4% ITD, 5% TKD.
CPT Codes
ITD: 81245
CODON: 81246
LOINC Mapping
Blood and Tissue: 92843-2(835) & 79210-1(ITD)
Bone Marrow: 85100-6
Order Type (Individual or Group)
G
Group Test Information
Result Test ID
Reportable
Result Test Name
Result Type
Type (Alpha or Numeric)
TYPM3
Y
SPECIMEN TYPE, FLT3
I
A
ITD
Y
ITD
I
A
CODON
Y
CODON 835/836
I
A
INTFT
Y
INTERPRETATION, FLT3
I
A
REVFT
Y
REVIEWED BY:
I
A
RRFLT
Y
FLT3 REVIEW
I
A
CPT Codes
ITD: 81245
CODON: 81246
LOINC Mapping
Blood and Tissue: 92843-2(835) & 79210-1(ITD)
Bone Marrow: 85100-6
Pricing
Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements
Collect
Heme samples : one 5 mL blood in EDTA (lav top), or one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells)
Fresh tissue: min 0.1mL
Frozen tissue: min 100mg or 0.5cc / 0.5 cm3
Body Fluids: >200K cells
Storage/Transport Temperature
20-25°C for several hours acceptable
Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.
Frozen samples should be sent on dry ice.
A positive result indicates the presence of FLT3 internal tandem duplication (ITD) or a tyrosine kinase domain (TKD) point mutation in codons 835 or 836. The limit of detection for this assay is 1 in 20 cells. Results of this test must always be interpreted in the context of clinicopathologic and relevant data, and should not be used alone for a diagnosis of malignancy. Methodology: Patient DNA is isolated, purified and subjected to PCR amplification using specific oligonucleotide primers directed at exons 14, 15, and 20 of the FLT3 gene. Each assay includes a positive control reaction using DNA from a specimen or cell line known to contain a FLT3-ITD mutation or exon 20 point mutation and a negative control reaction using DNA from a specimen or cell line containing the wild-type FLT3 sequence. Amplicons for exon 20 are digested with EcoR V. PCR products are separated by electrophoresis. The limit of detection for this assay is 4% ITD, 5% TKD.
Coding
CPT Codes
ITD: 81245
CODON: 81246
LOINC Mapping
Blood and Tissue: 92843-2(835) & 79210-1(ITD)
Bone Marrow: 85100-6
URM Labs Internal
Test Build
Order Type (Individual or Group)
G
Group Test Information
Result Test ID
Reportable
Result Test Name
Result Type
Type (Alpha or Numeric)
TYPM3
Y
SPECIMEN TYPE, FLT3
I
A
ITD
Y
ITD
I
A
CODON
Y
CODON 835/836
I
A
INTFT
Y
INTERPRETATION, FLT3
I
A
REVFT
Y
REVIEWED BY:
I
A
RRFLT
Y
FLT3 REVIEW
I
A
CPT Codes
ITD: 81245
CODON: 81246
LOINC Mapping
Blood and Tissue: 92843-2(835) & 79210-1(ITD)
Bone Marrow: 85100-6
Pricing
Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
