This qualitative Nucleic Acid Amplification Test is intended for the detection of DNA from Monkeypox Virus and non-Variola Orthopox Virus in vesicular or pustular swab specimens.
Collection Instructions
Collect cells from the base of a lesion by vigorously swabbing with a dry sterile synthetic swab and place that swab in transport media (UTM or VTM); swab is provided with tube of transport media. Vigorous swabbing of a lesion with both sides of a swab maximizes collection of an adequate specimen.
If a lesion ruptures while swabbing, ensure that lesion fluid is collected on the swab.
Place the swab in the tube of VTM and ensure that the tube cap is secured tightly to avoid leakage.
Label the tube with unique identifiers (Patient name, MRN, DOB, Specimen Collection Date, Specimen source). Place tubes in sealed ziplock bags and send to UR laboratory.
In EPIC, Order "Monkeypox and Non-Variola Orthopox NAAT" and indicate Specimen Source.
Storage/Transport Temperature
Transport the inoculated UTM to the lab at room temperature
Unacceptable Conditions
Unlabeled specimens
Leaking or non-sterile containers
Wooden swabs.
Swabs in inappropriate transport media
Unlabeled or expired transport media
Frozen samples
Specimens submitted without a source
Specimens outside stability
Stability (from collection to initiation)
Stable for 48 hours at ambient temperature (15-30°C).
Stable for 7 days at refrigerated temperature (2-8°C)
Collection device
UTM or VTM with flocked synthetic swab
Test Barcode Number
15227
Lab Section
Microbiology (Virology)
Methodology
Nucleic acid amplification (NAAT)
Performed
Mon - Sun
Reported
Within 24 hours of specimen receipt by the laboratory
Refer to Lab Account Manager. email: labservicesoutreach@urmc.rochester.edu
Specimen Requirements
Ordering Recommendations
This qualitative Nucleic Acid Amplification Test is intended for the detection of DNA from Monkeypox Virus and non-Variola Orthopox Virus in vesicular or pustular swab specimens.
Collection Instructions
Collect cells from the base of a lesion by vigorously swabbing with a dry sterile synthetic swab and place that swab in transport media (UTM or VTM); swab is provided with tube of transport media. Vigorous swabbing of a lesion with both sides of a swab maximizes collection of an adequate specimen.
If a lesion ruptures while swabbing, ensure that lesion fluid is collected on the swab.
Place the swab in the tube of VTM and ensure that the tube cap is secured tightly to avoid leakage.
Label the tube with unique identifiers (Patient name, MRN, DOB, Specimen Collection Date, Specimen source). Place tubes in sealed ziplock bags and send to UR laboratory.
In EPIC, Order "Monkeypox and Non-Variola Orthopox NAAT" and indicate Specimen Source.
Storage/Transport Temperature
Transport the inoculated UTM to the lab at room temperature
Unacceptable Conditions
Unlabeled specimens
Leaking or non-sterile containers
Wooden swabs.
Swabs in inappropriate transport media
Unlabeled or expired transport media
Frozen samples
Specimens submitted without a source
Specimens outside stability
Stability (from collection to initiation)
Stable for 48 hours at ambient temperature (15-30°C).
Stable for 7 days at refrigerated temperature (2-8°C)
Collection device
UTM or VTM with flocked synthetic swab
Test Barcode Number
15227
Testing
Lab Section
Microbiology (Virology)
Methodology
Nucleic acid amplification (NAAT)
Performed
Mon - Sun
Reported
Within 24 hours of specimen receipt by the laboratory
