Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM.
Fresh tissue: min 0.1mL, 0.1 cc
Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Blood samples in yellow top (ACD) Vacutainers are also acceptable.
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity. Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.
Unacceptable Conditions
No tumor tissue. Frozen specimens and specimens fixed/processed in alternative fixatives.
Stability (from collection to initiation)
Fresh Samples: Ambient: 72 hours; Refrigerated: 1 week
FFPE Samples Ambient: 60 days
Protect FFPE samples from heat exposure, especially in summer months. Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.
Formalin-Fixed, paraffin-embedded tissue (FFPE) in microtainer: 3-5 10uM slices or >100K cells
Formalin-Fixed, paraffin-embedded tissue (FFPE) slides: 10 unstained slides with slices of 10uM.
Fresh tissue: min 0.1mL, 0.1 cc
Heme samples : one 5 mL blood in EDTA (lav top), OR one 3 mL bone marrow in EDTA (min 0.5mL or >100K nucleated cells). Blood samples in yellow top (ACD) Vacutainers are also acceptable.
Body Fluids: >200K cells
Fine needle aspirate slides: Minimum volume is dependent on the tumor size and cellularity. Unfixed, ethanol fixed, or H&E stained – Soak slide in xylene to remove coverslip and dry before transporting to the lab.
Unacceptable Conditions
No tumor tissue. Frozen specimens and specimens fixed/processed in alternative fixatives.
Stability (from collection to initiation)
Fresh Samples: Ambient: 72 hours; Refrigerated: 1 week
FFPE Samples Ambient: 60 days
Protect FFPE samples from heat exposure, especially in summer months. Fresh tissue should be sent in saline soaked gauze, a tube with saline, or tissue culture medium.
