Patient Preparation

NA

Collect

Nasopharyngeal swab.  Place in 1-2 mL universal viral transport media.   

Unacceptable Conditions

BAL, sputum, tracheal aspirates, and throat swabs.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Room temperature:  stable for 4 hours
Refigerated:  stable for 3 days
Frozen:  stable for 30 days

Performed

Microbiology Department

Test performed daily on all shifts.  Reported on same shift received.  During peak season, results will be available within 24 hours of receipt.

Performed

Microbiology Department

Test performed daily on all shifts.  Reported on same shift received.  During peak season, results will be available within 24 hours of receipt.

Methodology

PCR

Reference Interval

Reported as Detected, Not Detected or Equivocal
 

Interpretive Data

The Microbiology Clinical Laboratory implemented a change in methodology for respiratory viral testing by PCR. The respiratory viral molecular test has been changed to the Biofire FilmArray Respiratory Pathogen Panel (RESPP). The RESPP is a multiplexed molecular test that will qualitatively detect and identify different respiratory pathogens simultaneously. The respiratory viruses detected include adenovirus, coronavirus (229E, OC43, NL63 and HKU1), SARS-CoV-2, Influenza A virus (with subtyping for H1, 2009 H1 and H3), Influenza B virus, human metapneumovirus, parainfluenza virus (1, 2, 3, and 4), human rhinovirus/enterovirus, and respiratory syncytial virus (RSV). The bacterial pathogens detected are Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae. 
Please note the direct antigen tests for influenza type A/type B and RSV are available for rapid screening when clinically indicated (Influenza type A & type B Antigen Detection; RSV Direct Antigen Detection).
 

CPT Codes

0202U

Test Build Information

OrderCode OrderName ResultCode ResultName Result Units LOINC CPT
RESPP RESP PATH PANEL PCR ADEN ADENOVIRUS   82160-3 0202U
RESPP RESP PATH PANEL PCR CV229E CORONAVIRUS 229E   82163-7  
RESPP RESP PATH PANEL PCR CVHKU1 CORONAVIRUS HKU1   82161-1  
RESPP RESP PATH PANEL PCR CVNL63 CORONAVIRUS NL63   82162-9  
RESPP RESP PATH PANEL PCR CVOC43 CORONAVIRUS OC43   82164-5  
RESPP RESP PATH PANEL PCR SARSV2 SARS-CoV-2   94565-9  
RESPP RESP PATH PANEL PCR HMETAP METAPNEUMOVIRUS   82165-2  
RESPP RESP PATH PANEL PCR HRHENT RHINOVIRUS/ENTEROVIRUS   82175-1  
RESPP RESP PATH PANEL PCR IVA INFLUENZA TYPE A   82166-0  
RESPP RESP PATH PANEL PCR IV2009 INFLU 2009A-H1N1v   82168-6  
RESPP RESP PATH PANEL PCR IVAH1 INFLU A SUBTYPE H1   82167-8  
RESPP RESP PATH PANEL PCR IVAH3 INFLU A SUBTYPE H3   82169-4  
RESPP RESP PATH PANEL PCR IVB INFLUENZA TYPE B   82170-2  
RESPP RESP PATH PANEL PCR PARAV1 PARAINFLUENZA 1   82171-0  
RESPP RESP PATH PANEL PCR PARAV2 PARAINFLUENZA 2   82172-8  
RESPP RESP PATH PANEL PCR PARAV3 PARAINFLUENZA 3   82173-6  
RESPP RESP PATH PANEL PCR PARAV4 PARAINFLUENZA 4   82174-4  
RESPP RESP PATH PANEL PCR RESYV RESP SYNCYTIAL VIRUS   82176-9  
RESPP RESP PATH PANEL PCR BORPER BORDETELLA PERTUSSIS   82179-3  
RESPP RESP PATH PANEL PCR BORPAR BORDETELLA PARAPERTUSSIS (IS1001)   87621-9  
RESPP RESP PATH PANEL PCR CHLPNE CHLAMYDOPHILA PNEUMONIAE   82178-5  
RESPP RESP PATH PANEL PCR MYCPNE MYCOPLASMA PNEUMONIAE   82177-7  
RESPP RESP PATH PANEL PCR RVPSRC Source   31208-2  
RESPP RESP PATH PANEL PCR RVPCOM Comment      

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 
Collection

Patient Preparation

NA

Collect

Nasopharyngeal swab.  Place in 1-2 mL universal viral transport media.   

Unacceptable Conditions

BAL, sputum, tracheal aspirates, and throat swabs.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

Room temperature:  stable for 4 hours
Refigerated:  stable for 3 days
Frozen:  stable for 30 days

Performed

Microbiology Department

Test performed daily on all shifts.  Reported on same shift received.  During peak season, results will be available within 24 hours of receipt.

Ordering

Performed

Microbiology Department

Test performed daily on all shifts.  Reported on same shift received.  During peak season, results will be available within 24 hours of receipt.

Methodology

PCR

Result Interpretation

Reference Interval

Reported as Detected, Not Detected or Equivocal
 

Interpretive Data

The Microbiology Clinical Laboratory implemented a change in methodology for respiratory viral testing by PCR. The respiratory viral molecular test has been changed to the Biofire FilmArray Respiratory Pathogen Panel (RESPP). The RESPP is a multiplexed molecular test that will qualitatively detect and identify different respiratory pathogens simultaneously. The respiratory viruses detected include adenovirus, coronavirus (229E, OC43, NL63 and HKU1), SARS-CoV-2, Influenza A virus (with subtyping for H1, 2009 H1 and H3), Influenza B virus, human metapneumovirus, parainfluenza virus (1, 2, 3, and 4), human rhinovirus/enterovirus, and respiratory syncytial virus (RSV). The bacterial pathogens detected are Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae. 
Please note the direct antigen tests for influenza type A/type B and RSV are available for rapid screening when clinically indicated (Influenza type A & type B Antigen Detection; RSV Direct Antigen Detection).
 

Administrative

CPT Codes

0202U
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName Result Units LOINC CPT
RESPP RESP PATH PANEL PCR ADEN ADENOVIRUS   82160-3 0202U
RESPP RESP PATH PANEL PCR CV229E CORONAVIRUS 229E   82163-7  
RESPP RESP PATH PANEL PCR CVHKU1 CORONAVIRUS HKU1   82161-1  
RESPP RESP PATH PANEL PCR CVNL63 CORONAVIRUS NL63   82162-9  
RESPP RESP PATH PANEL PCR CVOC43 CORONAVIRUS OC43   82164-5  
RESPP RESP PATH PANEL PCR SARSV2 SARS-CoV-2   94565-9  
RESPP RESP PATH PANEL PCR HMETAP METAPNEUMOVIRUS   82165-2  
RESPP RESP PATH PANEL PCR HRHENT RHINOVIRUS/ENTEROVIRUS   82175-1  
RESPP RESP PATH PANEL PCR IVA INFLUENZA TYPE A   82166-0  
RESPP RESP PATH PANEL PCR IV2009 INFLU 2009A-H1N1v   82168-6  
RESPP RESP PATH PANEL PCR IVAH1 INFLU A SUBTYPE H1   82167-8  
RESPP RESP PATH PANEL PCR IVAH3 INFLU A SUBTYPE H3   82169-4  
RESPP RESP PATH PANEL PCR IVB INFLUENZA TYPE B   82170-2  
RESPP RESP PATH PANEL PCR PARAV1 PARAINFLUENZA 1   82171-0  
RESPP RESP PATH PANEL PCR PARAV2 PARAINFLUENZA 2   82172-8  
RESPP RESP PATH PANEL PCR PARAV3 PARAINFLUENZA 3   82173-6  
RESPP RESP PATH PANEL PCR PARAV4 PARAINFLUENZA 4   82174-4  
RESPP RESP PATH PANEL PCR RESYV RESP SYNCYTIAL VIRUS   82176-9  
RESPP RESP PATH PANEL PCR BORPER BORDETELLA PERTUSSIS   82179-3  
RESPP RESP PATH PANEL PCR BORPAR BORDETELLA PARAPERTUSSIS (IS1001)   87621-9  
RESPP RESP PATH PANEL PCR CHLPNE CHLAMYDOPHILA PNEUMONIAE   82178-5  
RESPP RESP PATH PANEL PCR MYCPNE MYCOPLASMA PNEUMONIAE   82177-7  
RESPP RESP PATH PANEL PCR RVPSRC Source   31208-2  
RESPP RESP PATH PANEL PCR RVPCOM Comment      

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu