Collect

Blood. Draw aseptically per Blood Culture Collection procedure. Wipe off top of Blood culture bottles with alcohol wipe and let dry. Collect blood into Bactec Plus Aerobic /F bottle and Bactec Lytic/Anaerobic F bottle.
3.0-10.0 mL of blood into each Bactec aerobic bottle and Bactec anaerobic bottle. Optimum blood specimen is 20 mL.
If less blood is obtained, split the amount of blood drawn between bottles, minimum 3mL required for each bottle.
For Pediatric patients, refer to pediatric collection chart for volume of blood based on weight - Ordered as a BLDA.
                                                                                                                                    Blood per bottle
Order                                  BACTEC Bottle/Label Color                                    Maximum  Minimum         
Aerobic/Anaerobic                    Plus Aerobic/F (gray)                                                 10mL           3mL
Blood culture                          Lytic/Anaerobic/F (purple)                                            10mL           3mL
(BLDCU)
                 
Blood Culture Collection Procedure

Pediatric Collection

For Pediatric Patients only - volume of blood collection based on weight.  Order BLDA.

   For Multiple Draw Sites:
Patient Dosing Weight Total Volume
(ml)		 Pediatric Vial (Pink)
Line site/ Peripheral site
(ml)		 Regular Vial (Blue)
Line site/ Peripheral site
(ml)
3.9 kg or less 2 1/1  
4-7.9 kg 3 1.5/1.5  
8-13.9 kg 6 3/3  
14-18.9 kg 12   6/6
19-25.9 kg 16   8/8
26-39.9 kg 20   10/10
40kg or greater 40   2sets per adult protocol










           

  For a single draw site with entire volume <10mL, place entire volume into 1 bottle
Patient Dosing Weight Total Volume
(ml)		 Pediatric Vial (Pink)
All from same site
(ml)		 Regular Vial (Blue)
Line site/ Peripheral site
(ml)
3.9 kg or less 1 1  
4-7.9 kg 3 3  
8-13.9 kg 6   6 (adult bottle)

 

Unacceptable Conditions

Refrigerated BACTEC bottles

Blood cultures not collected in BACTEC bottles

Storage/Transport Temperature

Transport to lab immediately at 20-25° C.

Performed

Microbiology.
Daily.

Remarks

State source of blood if other than peripheral, for example a line draw, on the test request form. No more than 3 blood cultures sets should be drawn within a 24 hour period, unless from multiples lines. Note if patient is suspected of having Brucella, endocarditis or any other unusual organism.

Initial positive cultures will be reflexed by the lab to the Direct Blood Pathogen panel for a rapid, PCR-based, identification of the organism(s).

Notes

2024 Nationwide Shortage of Bactec Blood Culture Bottles Guidance:

Performed

Microbiology.
Daily.

Methodology

Continuous monitoring system. Standard reference procedures for identification of aerobic and anaerobic microorganisms and yeast.

Reported

Preliminary - no growth at 1 day.
Final - no growth at 5 days.
All positive blood cultures are reported as soon as detected.

Synonyms

  • Blood Culture, Aerobic and Anaerobic

Reference Interval

No growth at 5 days.

CPT Codes

87040

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
BLDCU BLOOD CULTURE SDES Specimen Source   31208-2 87040
BLDCU BLOOD CULTURE SREQ Additional Info   48767-8  
BLDCU BLOOD CULTURE CULT Culture Result:   41852-5  
BLDCU BLOOD CULTURE RPT Report Status      

If Aerobic only, use the below code: 
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
BLDA BLOOD CULT AEROBIC SDES Specimen Source   31208-2 87040
BLDA BLOOD CULT AEROBIC SREQ Additional Info   48767-8  
BLDA BLOOD CULT AEROBIC CULT Culture Result:   41852-5  
BLDA BLOOD CULT AEROBIC RPT Report Status      

Reflex BCID2 on first positive blood Culture:
 
OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
BCID2 BLOOD PATH PANEL PCR CTXM CTX-M   88250-6 87154
BCID2 BLOOD PATH PANEL PCR IMPB IMP   85498-4  
BCID2 BLOOD PATH PANEL PCR KPC KPC   49617-4  
BCID2 BLOOD PATH PANEL PCR MCR1 mcr-1   86221-9  
BCID2 BLOOD PATH PANEL PCR MECAC mecA/C   92968-7  
BCID2 BLOOD PATH PANEL PCR MECACM mecA/c + MREJ (MRSA)   96309-0  
BCID2 BLOOD PATH PANEL PCR NDMB NDM   73982-1  
BCID2 BLOOD PATH PANEL PCR OXA48 OXA-48-like   85827-4  
BCID2 BLOOD PATH PANEL PCR VANAB vanA/B   62261-3  
BCID2 BLOOD PATH PANEL PCR VIM VIM   85501-5  
BCID2 BLOOD PATH PANEL PCR EFAES Entero. faecalis   96304-1  
BCID2 BLOOD PATH PANEL PCR EFAEM Entero. faecium   96305-8  
BCID2 BLOOD PATH PANEL PCR LIMON L. monocytogenes   85763-1  
BCID2 BLOOD PATH PANEL PCR STAPHY Staphylococcus genus   85764-9  
BCID2 BLOOD PATH PANEL PCR STAUR Staph aureus   85765-6  
BCID2 BLOOD PATH PANEL PCR SEPID Staph. epidermidis   96310-8  
BCID2 BLOOD PATH PANEL PCR SLUGD Staph. lugdunensis   96311-6  
BCID2 BLOOD PATH PANEL PCR STREPT Streptococcus genus   85766-4  
BCID2 BLOOD PATH PANEL PCR STAGA Strep agalactiae   85767-2  
BCID2 BLOOD PATH PANEL PCR STPNE Strep pneumoniae   85768-0  
BCID2 BLOOD PATH PANEL PCR STPYO Strep pyogenes   85769-8  
BCID2 BLOOD PATH PANEL PCR ACBAU A. baumanii complex   85770-6  
BCID2 BLOOD PATH PANEL PCR BFRAG Bacteroides fragilis   96301-7  
BCID2 BLOOD PATH PANEL PCR ENTBAC Enterobacterales   96306-6  
BCID2 BLOOD PATH PANEL PCR ENCLO E. cloacae complex   85772-2  
BCID2 BLOOD PATH PANEL PCR ESCOL Escherichia coli   85773-0  
BCID2 BLOOD PATH PANEL PCR KAERO Klebsiella aerogenes   96308-2  
BCID2 BLOOD PATH PANEL PCR KLOXY Kleb oxytoca   85774-8  
BCID2 BLOOD PATH PANEL PCR KLPNE Kleb pneumoniae   96307-4  
BCID2 BLOOD PATH PANEL PCR PROTE Proteus spp.   85776-3  
BCID2 BLOOD PATH PANEL PCR SALMSP Salmonella spp.   96313-2  
BCID2 BLOOD PATH PANEL PCR SERRA S. marcescens   85777-1  
BCID2 BLOOD PATH PANEL PCR HAINF H. influenzae   85778-9  
BCID2 BLOOD PATH PANEL PCR NEMEN N. meningitidis   85779-7  
BCID2 BLOOD PATH PANEL PCR PSEAER Ps. aeruginosa   85780-5  
BCID2 BLOOD PATH PANEL PCR SMALTO Steno. maltophilia   96312-4  
BCID2 BLOOD PATH PANEL PCR CAALB Candida albicans   85781-3  
BCID2 BLOOD PATH PANEL PCR CAURIS Candida auris   96302-5  
BCID2 BLOOD PATH PANEL PCR CAGLA Candida glabrata   85782-1  
BCID2 BLOOD PATH PANEL PCR CAKRU Candida krusei   85783-9  
BCID2 BLOOD PATH PANEL PCR CAPAR Candida parapsilosis   85784-7  
BCID2 BLOOD PATH PANEL PCR CATRO Candida tropicalis   85785-4  
BCID2 BLOOD PATH PANEL PCR CNEOF C. neoformans/gattii   96303-3  
BCID2 BLOOD PATH PANEL PCR BCIDCM Comments      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
BLDA BLOOD CULT AEROBIC SDES Specimen Source Blood   BLUD List
BLDA BLOOD CULT AEROBIC     Blood Arterial   BLAR  
BLDA BLOOD CULT AEROBIC     Blood Capillary   BLCP  
BLDA BLOOD CULT AEROBIC     Blood Venous   BLDV  
BLDA BLOOD CULT AEROBIC     Blood, Line Draw   BLLN  
BLDA BLOOD CULT AEROBIC     Blood, Peripheral Draw   BLPH  

Example Report

Sample Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 
Collection

Collect

Blood. Draw aseptically per Blood Culture Collection procedure. Wipe off top of Blood culture bottles with alcohol wipe and let dry. Collect blood into Bactec Plus Aerobic /F bottle and Bactec Lytic/Anaerobic F bottle.
3.0-10.0 mL of blood into each Bactec aerobic bottle and Bactec anaerobic bottle. Optimum blood specimen is 20 mL.
If less blood is obtained, split the amount of blood drawn between bottles, minimum 3mL required for each bottle.
For Pediatric patients, refer to pediatric collection chart for volume of blood based on weight - Ordered as a BLDA.
                                                                                                                                    Blood per bottle
Order                                  BACTEC Bottle/Label Color                                    Maximum  Minimum         
Aerobic/Anaerobic                    Plus Aerobic/F (gray)                                                 10mL           3mL
Blood culture                          Lytic/Anaerobic/F (purple)                                            10mL           3mL
(BLDCU)
                 
Blood Culture Collection Procedure

Pediatric Collection

For Pediatric Patients only - volume of blood collection based on weight.  Order BLDA.

   For Multiple Draw Sites:
Patient Dosing Weight Total Volume
(ml)		 Pediatric Vial (Pink)
Line site/ Peripheral site
(ml)		 Regular Vial (Blue)
Line site/ Peripheral site
(ml)
3.9 kg or less 2 1/1  
4-7.9 kg 3 1.5/1.5  
8-13.9 kg 6 3/3  
14-18.9 kg 12   6/6
19-25.9 kg 16   8/8
26-39.9 kg 20   10/10
40kg or greater 40   2sets per adult protocol










           

  For a single draw site with entire volume <10mL, place entire volume into 1 bottle
Patient Dosing Weight Total Volume
(ml)		 Pediatric Vial (Pink)
All from same site
(ml)		 Regular Vial (Blue)
Line site/ Peripheral site
(ml)
3.9 kg or less 1 1  
4-7.9 kg 3 3  
8-13.9 kg 6   6 (adult bottle)

 

Unacceptable Conditions

Refrigerated BACTEC bottles

Blood cultures not collected in BACTEC bottles

Storage/Transport Temperature

Transport to lab immediately at 20-25° C.

Performed

Microbiology.
Daily.

Remarks

State source of blood if other than peripheral, for example a line draw, on the test request form. No more than 3 blood cultures sets should be drawn within a 24 hour period, unless from multiples lines. Note if patient is suspected of having Brucella, endocarditis or any other unusual organism.

Initial positive cultures will be reflexed by the lab to the Direct Blood Pathogen panel for a rapid, PCR-based, identification of the organism(s).

Notes

2024 Nationwide Shortage of Bactec Blood Culture Bottles Guidance:
Ordering

Performed

Microbiology.
Daily.

Methodology

Continuous monitoring system. Standard reference procedures for identification of aerobic and anaerobic microorganisms and yeast.

Reported

Preliminary - no growth at 1 day.
Final - no growth at 5 days.
All positive blood cultures are reported as soon as detected.

Synonyms

  • Blood Culture, Aerobic and Anaerobic
Result Interpretation

Reference Interval

No growth at 5 days.

Administrative

CPT Codes

87040
RPS Interface Information

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
BLDCU BLOOD CULTURE SDES Specimen Source   31208-2 87040
BLDCU BLOOD CULTURE SREQ Additional Info   48767-8  
BLDCU BLOOD CULTURE CULT Culture Result:   41852-5  
BLDCU BLOOD CULTURE RPT Report Status      

If Aerobic only, use the below code: 
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
BLDA BLOOD CULT AEROBIC SDES Specimen Source   31208-2 87040
BLDA BLOOD CULT AEROBIC SREQ Additional Info   48767-8  
BLDA BLOOD CULT AEROBIC CULT Culture Result:   41852-5  
BLDA BLOOD CULT AEROBIC RPT Report Status      

Reflex BCID2 on first positive blood Culture:
 
OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
BCID2 BLOOD PATH PANEL PCR CTXM CTX-M   88250-6 87154
BCID2 BLOOD PATH PANEL PCR IMPB IMP   85498-4  
BCID2 BLOOD PATH PANEL PCR KPC KPC   49617-4  
BCID2 BLOOD PATH PANEL PCR MCR1 mcr-1   86221-9  
BCID2 BLOOD PATH PANEL PCR MECAC mecA/C   92968-7  
BCID2 BLOOD PATH PANEL PCR MECACM mecA/c + MREJ (MRSA)   96309-0  
BCID2 BLOOD PATH PANEL PCR NDMB NDM   73982-1  
BCID2 BLOOD PATH PANEL PCR OXA48 OXA-48-like   85827-4  
BCID2 BLOOD PATH PANEL PCR VANAB vanA/B   62261-3  
BCID2 BLOOD PATH PANEL PCR VIM VIM   85501-5  
BCID2 BLOOD PATH PANEL PCR EFAES Entero. faecalis   96304-1  
BCID2 BLOOD PATH PANEL PCR EFAEM Entero. faecium   96305-8  
BCID2 BLOOD PATH PANEL PCR LIMON L. monocytogenes   85763-1  
BCID2 BLOOD PATH PANEL PCR STAPHY Staphylococcus genus   85764-9  
BCID2 BLOOD PATH PANEL PCR STAUR Staph aureus   85765-6  
BCID2 BLOOD PATH PANEL PCR SEPID Staph. epidermidis   96310-8  
BCID2 BLOOD PATH PANEL PCR SLUGD Staph. lugdunensis   96311-6  
BCID2 BLOOD PATH PANEL PCR STREPT Streptococcus genus   85766-4  
BCID2 BLOOD PATH PANEL PCR STAGA Strep agalactiae   85767-2  
BCID2 BLOOD PATH PANEL PCR STPNE Strep pneumoniae   85768-0  
BCID2 BLOOD PATH PANEL PCR STPYO Strep pyogenes   85769-8  
BCID2 BLOOD PATH PANEL PCR ACBAU A. baumanii complex   85770-6  
BCID2 BLOOD PATH PANEL PCR BFRAG Bacteroides fragilis   96301-7  
BCID2 BLOOD PATH PANEL PCR ENTBAC Enterobacterales   96306-6  
BCID2 BLOOD PATH PANEL PCR ENCLO E. cloacae complex   85772-2  
BCID2 BLOOD PATH PANEL PCR ESCOL Escherichia coli   85773-0  
BCID2 BLOOD PATH PANEL PCR KAERO Klebsiella aerogenes   96308-2  
BCID2 BLOOD PATH PANEL PCR KLOXY Kleb oxytoca   85774-8  
BCID2 BLOOD PATH PANEL PCR KLPNE Kleb pneumoniae   96307-4  
BCID2 BLOOD PATH PANEL PCR PROTE Proteus spp.   85776-3  
BCID2 BLOOD PATH PANEL PCR SALMSP Salmonella spp.   96313-2  
BCID2 BLOOD PATH PANEL PCR SERRA S. marcescens   85777-1  
BCID2 BLOOD PATH PANEL PCR HAINF H. influenzae   85778-9  
BCID2 BLOOD PATH PANEL PCR NEMEN N. meningitidis   85779-7  
BCID2 BLOOD PATH PANEL PCR PSEAER Ps. aeruginosa   85780-5  
BCID2 BLOOD PATH PANEL PCR SMALTO Steno. maltophilia   96312-4  
BCID2 BLOOD PATH PANEL PCR CAALB Candida albicans   85781-3  
BCID2 BLOOD PATH PANEL PCR CAURIS Candida auris   96302-5  
BCID2 BLOOD PATH PANEL PCR CAGLA Candida glabrata   85782-1  
BCID2 BLOOD PATH PANEL PCR CAKRU Candida krusei   85783-9  
BCID2 BLOOD PATH PANEL PCR CAPAR Candida parapsilosis   85784-7  
BCID2 BLOOD PATH PANEL PCR CATRO Candida tropicalis   85785-4  
BCID2 BLOOD PATH PANEL PCR CNEOF C. neoformans/gattii   96303-3  
BCID2 BLOOD PATH PANEL PCR BCIDCM Comments      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
BLDA BLOOD CULT AEROBIC SDES Specimen Source Blood   BLUD List
BLDA BLOOD CULT AEROBIC     Blood Arterial   BLAR  
BLDA BLOOD CULT AEROBIC     Blood Capillary   BLCP  
BLDA BLOOD CULT AEROBIC     Blood Venous   BLDV  
BLDA BLOOD CULT AEROBIC     Blood, Line Draw   BLLN  
BLDA BLOOD CULT AEROBIC     Blood, Peripheral Draw   BLPH  

Example Report

Sample Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 

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