7.0 mL or 5.0 mL EDTA (Lavender tube) tube to provide 1.5 mL plasma (minimum 700 uL plasma).
Centrifuge tube within 24 hours of collection, aliquot plasma and store frozen.
Unacceptable Conditions
Serum samples are unacceptable
Storage/Transport Temperature
Aliquot and refirgerate or freeze
Stability (from collection to initiation)
For EDTA plasma, whole blood can be stored for no longer than 24 hours at 2-25°C. Separate plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature.
Plasma may be stored at room temperature for up to 1 day, at 2-8°C for up to 6 days or frozen at -20°C to -80°C for up to 6 weeks.
Performed
Microbiology: Molecular Virology.
Test performed at least once/week.
Remarks
Centrifuge the sample and separate the plasma from the cells within 24 hours of collection. Transfer the plasma to a clean transport tube and freeze. Label tube with patient name, date of birth and 'EDTA Plasma'.
Notes
The result of "Not Detected" does not rule out the presence of HIV RNA below the level of detection. The quantitative range for his assay is 1.3-7.0 log copies/mL (20-10,000,000 copies/mL). If HIV RNA is detected below the quantitative range it will be reported as <20 copies/mL.
This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels.
The clinical significance of changes in HIV-1 RNA measurement has not been fully established; however, a threefold (0.5 log) change in copies/mL may be significant. False positive results may occur. Care should be taken when interpreting results especially for seronegative patients. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues, and Cellular Tissue-Based Products (HCT/P).
Performed
Microbiology: Molecular Virology.
Test performed at least once/week.
7.0 mL or 5.0 mL EDTA (Lavender tube) tube to provide 1.5 mL plasma (minimum 700 uL plasma).
Centrifuge tube within 24 hours of collection, aliquot plasma and store frozen.
Unacceptable Conditions
Serum samples are unacceptable
Storage/Transport Temperature
Aliquot and refirgerate or freeze
Stability (from collection to initiation)
For EDTA plasma, whole blood can be stored for no longer than 24 hours at 2-25°C. Separate plasma from whole blood within 24 hours of collection by centrifugation at 800-1600 x g for 20 minutes at room temperature.
Plasma may be stored at room temperature for up to 1 day, at 2-8°C for up to 6 days or frozen at -20°C to -80°C for up to 6 weeks.
Performed
Microbiology: Molecular Virology.
Test performed at least once/week.
Remarks
Centrifuge the sample and separate the plasma from the cells within 24 hours of collection. Transfer the plasma to a clean transport tube and freeze. Label tube with patient name, date of birth and 'EDTA Plasma'.
Notes
The result of "Not Detected" does not rule out the presence of HIV RNA below the level of detection. The quantitative range for his assay is 1.3-7.0 log copies/mL (20-10,000,000 copies/mL). If HIV RNA is detected below the quantitative range it will be reported as <20 copies/mL.
This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels.
The clinical significance of changes in HIV-1 RNA measurement has not been fully established; however, a threefold (0.5 log) change in copies/mL may be significant. False positive results may occur. Care should be taken when interpreting results especially for seronegative patients. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues, and Cellular Tissue-Based Products (HCT/P).
Ordering
Performed
Microbiology: Molecular Virology.
Test performed at least once/week.
