Collect

One 6.0 mL SST (Gold) tube or One PST tube or One 5.0 mL EDTA (Lavender) tube.
2 mL serum or plasma (Min: 1.0 mL).

Storage/Transport Temperature

Refrigerated. If sample transport to the lab is not the same day as collected; aliquot the serum or plasma.

Stability (from collection to initiation)

Stability: After separation from cells: Ambient: <8 hours; Refrigerated: 14 days if pour off serum, 72 hours if gel serum; Frozen: 1 year (avoid more than 6 freeze/thaw cycles)

Performed

Serology/Microbiology.
Daily.

Remarks

To aliquot: Centrifuge the sample and transfer the serum into a clean tube.

Notes

Report comment: Weakly reactive samples will be confirmed by neutralization. Specimen in positive range: Confirmatory testing is recommended on all reactive (positive) samples collected for prenatal screening. If patient fits this criteria, please call Regional Pathology Services to request confirmatory testing. Confirmation by Neutralization will be sent to ARUP Laboratories (#0020128).

Biotin interference:  Serum samples spiked with 10 ng/mL of biotin demonstrated <= 10% change in qualitative result.

Biotin Therapy may invalidate results. Some assays may exhibit interference when samples are collected from patients consuming higher than recommended doses of biotin or supplements (including prenatal multivitamins), which also contain biotin. Simple daily supplementation, recommended dosage, is not problematic. It is recommended that all patients be asked if they are consuming high-dose biotin or biotin containing supplements. Patients should be cautioned to stop using biotin-containing supplements at least 72 hours prior testing.
 

Performed

Serology/Microbiology.
Daily.

Methodology

Chemiluminescence

Reported

Results in 24 hours.

Reference Interval

Nonreactive

CPT Codes

87340

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
HBSAG HEPATITIS B/S AG HBSAG HEPATITIS B/S AG   5196-1 87340

Reflex if HBSAG is indeterminate:
OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
HBSCON HBSAG CONFIRM HBSCON HBSAG CONFIRM   65633-0 87341
 

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Collect

One 6.0 mL SST (Gold) tube or One PST tube or One 5.0 mL EDTA (Lavender) tube.
2 mL serum or plasma (Min: 1.0 mL).

Storage/Transport Temperature

Refrigerated. If sample transport to the lab is not the same day as collected; aliquot the serum or plasma.

Stability (from collection to initiation)

Stability: After separation from cells: Ambient: <8 hours; Refrigerated: 14 days if pour off serum, 72 hours if gel serum; Frozen: 1 year (avoid more than 6 freeze/thaw cycles)

Performed

Serology/Microbiology.
Daily.

Remarks

To aliquot: Centrifuge the sample and transfer the serum into a clean tube.

Notes

Report comment: Weakly reactive samples will be confirmed by neutralization. Specimen in positive range: Confirmatory testing is recommended on all reactive (positive) samples collected for prenatal screening. If patient fits this criteria, please call Regional Pathology Services to request confirmatory testing. Confirmation by Neutralization will be sent to ARUP Laboratories (#0020128).

Biotin interference:  Serum samples spiked with 10 ng/mL of biotin demonstrated <= 10% change in qualitative result.

Biotin Therapy may invalidate results. Some assays may exhibit interference when samples are collected from patients consuming higher than recommended doses of biotin or supplements (including prenatal multivitamins), which also contain biotin. Simple daily supplementation, recommended dosage, is not problematic. It is recommended that all patients be asked if they are consuming high-dose biotin or biotin containing supplements. Patients should be cautioned to stop using biotin-containing supplements at least 72 hours prior testing.
 

Ordering

Performed

Serology/Microbiology.
Daily.

Methodology

Chemiluminescence

Reported

Results in 24 hours.

Result Interpretation

Reference Interval

Nonreactive

Administrative

CPT Codes

87340
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
HBSAG HEPATITIS B/S AG HBSAG HEPATITIS B/S AG   5196-1 87340

Reflex if HBSAG is indeterminate:
OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
HBSCON HBSAG CONFIRM HBSCON HBSAG CONFIRM   65633-0 87341
 

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu