Patient Preparation

Patient should be fasting for at least 12-16 hours prior to drawing the sample. Water and prescribed medications are allowed.

Collect

Tube type:

   One 6.0 mL PST (Green) tube or

   One 6.0 mL SST (Gold) tube.


Volume Requirement:

   Optimum: 1.0 mL serum or plasma

   Minimum: 0.5 mL serum or plasma

(Microtainer- 1 full microtainer for LIPID panel)

Specimen Preparation

Serum: The gold stopper tube should be allowed to clot before centifuging. Specimens should be centrifuged at minimum 2500 RPM for  minimum 5 minutes. Serum should be separated from the cells promptly to an appropriately labeled tube, unless using SST tube.

Plasma: Mix anticoagulant with specimen adequately. Centrifuge at minimum 2500 RPM for minimu 5 minutes. Remove plasma promptly from cells to an appropriately labeled tube, unless using PST tube.

Storage/Transport Temperature

Centrifuge and remove serum or plasma from the cells within 2 hours of collection.

For transport greater than 8 hours, refrigerate or freeze sample. See below for component stabilities.

Cholesterol – Room temp for 8 hours; Refrigerated for 7 days; Frozen for 3 months
Triglyceride – Room temp for 8 hours; Refrigerated for 7 days; Frozen for 3 months
HDL – Room temp for 14 hours; Refrigerated for 5 days; Frozen for 3 months
LDL, VLDL and Cholesterol/HDL Ratio are calculated values and do not require storage instructions.
  

Performed

Chemistry.
Daily.

Remarks

Panel includes: Total Cholesterol, triglycerides, HDL Cholesterol, calculated LDL and VLDL and a calculated Cholesterol/HDL ratio.

Do not use sodium citrate or sodium NaF/potassium oxalate as anticoagulants.

Notes

The formulas for calculation are:

Very Low Density Lipoprotein (VLDL)=Triglyceride/5

Low Density Lipoprotein (LDL)=Cholesterol - (HDL + VLDL)

Performed

Chemistry.
Daily.

Methodology

Instrument: Beckman Coulter AU5800

Reported

Results same day.

Reference Interval

CHOLESTEROL
Adults, > 20 years, serum/plasma 100-199 mg/dL Desirable
  200-239 mg/dL Borderline High
  > 240 mg/dL High
Children, 1-19 years, serum/plasma 100-169 mg/dL Desirable
  170-199 mg/dL Borderline High
  > 200 mg/dL High
Children, <1 year, serum/plasma <170 mg/dL Desirable
TRIGLYCERIDE
ADULTS  > 15 YEARS
Desirable 0-149 mg/dL
Borderline High 150-199 mg/dL
High 200-499 mg/dL
Very High > 500 mg/dL
CHILDREN, <15 years
Desirable 0-125 mg/dL
HDL
ADULT,  >18 years
Adult Males
Low <40 mg/dL
Borderline Low 40-59 mg/dL
Desirable >59 mg/dL
Adult Females
Low <50 mg/dL
Borderline Low 50-59 mg/dL
Desirable >59 mg/dL
CHILDREN, 2-17 YEARS
Low <40 mg/dL
Borderline Low 40-45 mg/dL
Desirable >45 mg/dL
LDL
ADULTS
Optimal <100 mg/dL
Near or above Optimal 100-129 mg/dL
Borderline High 130-159 mg/dL
High 160-189 mg/dL
Very High > 190 mg/dL
CHILDREN
Desirable <110 mg/dL
Borderline High 110-129 mg/dL
High > 130 mg/dL
   

CPT Codes

80061

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
LIPID LIPID PANEL CHOL CHOLESTEROL mg/dL 2093-3 80061
LIPID LIPID PANEL TRIG TRIGLYCERIDE mg/dL 2571-8  
LIPID LIPID PANEL HDL HDL CHOLESTEROL mg/dL 2085-9  
LIPID LIPID PANEL LDL LDL CHOL CALCULATED mg/dL 13457-7  
LIPID LIPID PANEL VLDL VLDL CHOL CALCULATED mg/dL 13458-5  
LIPID LIPID PANEL RATIO CHOL/HDL RATIO   9830-1  

Example Report

Sample Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Patient Preparation

Patient should be fasting for at least 12-16 hours prior to drawing the sample. Water and prescribed medications are allowed.

Collect

Tube type:

   One 6.0 mL PST (Green) tube or

   One 6.0 mL SST (Gold) tube.


Volume Requirement:

   Optimum: 1.0 mL serum or plasma

   Minimum: 0.5 mL serum or plasma

(Microtainer- 1 full microtainer for LIPID panel)

Specimen Preparation

Serum: The gold stopper tube should be allowed to clot before centifuging. Specimens should be centrifuged at minimum 2500 RPM for  minimum 5 minutes. Serum should be separated from the cells promptly to an appropriately labeled tube, unless using SST tube.

Plasma: Mix anticoagulant with specimen adequately. Centrifuge at minimum 2500 RPM for minimu 5 minutes. Remove plasma promptly from cells to an appropriately labeled tube, unless using PST tube.

Storage/Transport Temperature

Centrifuge and remove serum or plasma from the cells within 2 hours of collection.

For transport greater than 8 hours, refrigerate or freeze sample. See below for component stabilities.

Cholesterol – Room temp for 8 hours; Refrigerated for 7 days; Frozen for 3 months
Triglyceride – Room temp for 8 hours; Refrigerated for 7 days; Frozen for 3 months
HDL – Room temp for 14 hours; Refrigerated for 5 days; Frozen for 3 months
LDL, VLDL and Cholesterol/HDL Ratio are calculated values and do not require storage instructions.
  

Performed

Chemistry.
Daily.

Remarks

Panel includes: Total Cholesterol, triglycerides, HDL Cholesterol, calculated LDL and VLDL and a calculated Cholesterol/HDL ratio.

Do not use sodium citrate or sodium NaF/potassium oxalate as anticoagulants.

Notes

The formulas for calculation are:

Very Low Density Lipoprotein (VLDL)=Triglyceride/5

Low Density Lipoprotein (LDL)=Cholesterol - (HDL + VLDL)

Ordering

Performed

Chemistry.
Daily.

Methodology

Instrument: Beckman Coulter AU5800

Reported

Results same day.
Result Interpretation

Reference Interval

CHOLESTEROL
Adults, > 20 years, serum/plasma 100-199 mg/dL Desirable
  200-239 mg/dL Borderline High
  > 240 mg/dL High
Children, 1-19 years, serum/plasma 100-169 mg/dL Desirable
  170-199 mg/dL Borderline High
  > 200 mg/dL High
Children, <1 year, serum/plasma <170 mg/dL Desirable
TRIGLYCERIDE
ADULTS  > 15 YEARS
Desirable 0-149 mg/dL
Borderline High 150-199 mg/dL
High 200-499 mg/dL
Very High > 500 mg/dL
CHILDREN, <15 years
Desirable 0-125 mg/dL
HDL
ADULT,  >18 years
Adult Males
Low <40 mg/dL
Borderline Low 40-59 mg/dL
Desirable >59 mg/dL
Adult Females
Low <50 mg/dL
Borderline Low 50-59 mg/dL
Desirable >59 mg/dL
CHILDREN, 2-17 YEARS
Low <40 mg/dL
Borderline Low 40-45 mg/dL
Desirable >45 mg/dL
LDL
ADULTS
Optimal <100 mg/dL
Near or above Optimal 100-129 mg/dL
Borderline High 130-159 mg/dL
High 160-189 mg/dL
Very High > 190 mg/dL
CHILDREN
Desirable <110 mg/dL
Borderline High 110-129 mg/dL
High > 130 mg/dL
   
Administrative

CPT Codes

80061
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
LIPID LIPID PANEL CHOL CHOLESTEROL mg/dL 2093-3 80061
LIPID LIPID PANEL TRIG TRIGLYCERIDE mg/dL 2571-8  
LIPID LIPID PANEL HDL HDL CHOLESTEROL mg/dL 2085-9  
LIPID LIPID PANEL LDL LDL CHOL CALCULATED mg/dL 13457-7  
LIPID LIPID PANEL VLDL VLDL CHOL CALCULATED mg/dL 13458-5  
LIPID LIPID PANEL RATIO CHOL/HDL RATIO   9830-1  

Example Report

Sample Report

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu

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