Patient Preparation

Collect specimen at 0800 AM.
Collections allowed one hour prior and one hour after 0800 AM.

Collect

Tube type:

   One 6.0 mL PST (Green) tube or

   One 6.0 mL SST (Gold) tube.


Volume Requirement:

   Optimum: 0.5 mL

   Minimum: 0.3 mL

Specimen Preparation

Serum: The red or gold stopper tube should be allowed to clot before centifuging. Specimens should be centrifuged at minimum 2500 RPM for minimum 5 minutes. Serum should be separated from the cells promptly to an appropriately labeled tube, unless using SST tube.

Plasma: Mix anticoagulant with specimen adequately. Specimens should be centrifuged at minimum 2500 RPM for minimum 5 minutes. Remove plasma promptly from cells to an appropriately labeled tube, unless using PST tube.

Storage/Transport Temperature

Centrifuge and remove serum or plasma from the cells with 2 hours of collection.

Storage:

   Plasma: Room temperature for 8 hours

                Refrigerator temperature for 0-48 hours

                Freezer temperature for >48 hours  

Serum: Room temperature for 8 hours

               Refrigerator temperature for 0-48 hours

               Freezer temperature for >48 hours

Performed

Chemistry.
Daily.

Performed

Chemistry.
Daily.

Methodology

Instrument: Beckman Coulter DxI 800

Reported

Results same day.

Reference Interval

Cortisol, 0800 AM: 6.7-22.6 ug/dL

CPT Codes

82533

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
COR8A CORTISOL/8 AM COR8A CORTISOL/8 AM mcg/dL 48104-4 82533

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Patient Preparation

Collect specimen at 0800 AM.
Collections allowed one hour prior and one hour after 0800 AM.

Collect

Tube type:

   One 6.0 mL PST (Green) tube or

   One 6.0 mL SST (Gold) tube.


Volume Requirement:

   Optimum: 0.5 mL

   Minimum: 0.3 mL

Specimen Preparation

Serum: The red or gold stopper tube should be allowed to clot before centifuging. Specimens should be centrifuged at minimum 2500 RPM for minimum 5 minutes. Serum should be separated from the cells promptly to an appropriately labeled tube, unless using SST tube.

Plasma: Mix anticoagulant with specimen adequately. Specimens should be centrifuged at minimum 2500 RPM for minimum 5 minutes. Remove plasma promptly from cells to an appropriately labeled tube, unless using PST tube.

Storage/Transport Temperature

Centrifuge and remove serum or plasma from the cells with 2 hours of collection.

Storage:

   Plasma: Room temperature for 8 hours

                Refrigerator temperature for 0-48 hours

                Freezer temperature for >48 hours  

Serum: Room temperature for 8 hours

               Refrigerator temperature for 0-48 hours

               Freezer temperature for >48 hours

Performed

Chemistry.
Daily.
Ordering

Performed

Chemistry.
Daily.

Methodology

Instrument: Beckman Coulter DxI 800

Reported

Results same day.
Result Interpretation

Reference Interval

Cortisol, 0800 AM: 6.7-22.6 ug/dL
Administrative

CPT Codes

82533
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
COR8A CORTISOL/8 AM COR8A CORTISOL/8 AM mcg/dL 48104-4 82533

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu