Collect

Serum separator tube. Postimmunization specimen should be drawn 30 days after immunization and, if shipped separately, must be received within 60 days of preimmunization specimen.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP standard transport tube. (Min: 0.25 mL) MARK SPECIMENS CLEARLY AS "PRE" OR "POST" SO SPECIMENS WILL BE SAVED AND TESTED SIMULTANEOUSLY.

Unacceptable Conditions

Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 60 days (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Referral Lab

Laboratories.

Ordering Recommendations

Use to evaluate antibody production and rule out antibody deficiency in patients vaccinated with a pure polysaccharide vaccine (eg, Pneumovax) or protein conjugated vaccine (eg, Prevnar or Vaxneuvance).

Performed

Sun-Sat

Methodology

Quantitative Multiplex Chemiluminescent Immunoassay

Reported

1-3 days

Synonyms

  • Immune Response
  • pneumo conjugate vaccine
  • Pneumo polysaccharide vaccine
  • Strep Pneumo Antibodies
  • Strep Pneumoniae Antibody
  • Strep Vaccine
  • Pneumococcal Antibodies
  • S. Pneumoniae Vaccine
  • Strep Antibodies 14 serotypes
  • Streptococcus pneumoniae Immune Response
  • Streptococcus pneumoniae Vaccine Response
  • Vaccine Response

ARUP Test Code

0050725

Interpretive Data

A pre- and postvaccination comparison is required to adequately assess the humoral immune response to the pure polysaccharide Pneumovax 23 (PNX) and/or the protein conjugated Prevnar 7 (P7), Prevnar 13 (P13), Prevnar 20 (P20), and Vaxneuvance (V15) Streptococcus pneumoniae vaccines. Prevaccination samples should be collected prior to vaccine administration. Postvaccination samples should be obtained at least 4 weeks after immunization. Testing of postvaccination samples alone will provide only general immune status of the individual to various pneumococcal serotypes.

In the case of pure polysaccharide vaccine, indication of immune system competence is further delineated as an adequate response to at least 50 percent of the serotypes in the vaccine challenge for those 2-5 years of age and to at least 70 percent of the serotypes in the vaccine challenge for those 6-65 years of age. Individual immune response may vary based on age, past exposure, immunocompetence, and pneumococcal serotype.

Responder Status                    Antibody Ratio

Nonresponder . . . . . . . . . . .  Less than twofold increase and postvaccination concentration less than 1.3 ug/mL

Good responder . . . . . . . . . . .At least a twofold increase and/or a postvaccination concentration greater than or equal to 1.3 ug/mL

A response to 50-70 percent or more of the serotypes in the vaccine challenge is considered a normal humoral response. (Daly, 2014) Antibody concentration greater than 1.0-1.3 ug/mL is generally considered long-term protection. (Daly, 2015)

References:

1. Daly TM, Pickering JW, Zhang X, et al. Multilaboratory assessment of threshold versus fold-change algorithms for minimizing analytical variability in multiplexed pneumococcal IgG measurements. Clin Vaccine Immunol. 2014;21(7):982-988.

2. Daly TM, Hill HR. Use and clinical interpretation of pneumococcal antibody measurements in the evaluation of humoral immune function. Clin Vaccine Immunol. 2015;22(2):148-152.

CPT Codes

86317 x14

LOINC

  • 85954-6
  • 86107-0
  • 86130-2
  • 27118-9
  • 86080-9
  • 25296-5
  • 86169-0
  • 85991-8
  • 42771-6
  • 86147-6
  • 30153-1
  • 85977-7
  • 27395-3
  • 86024-7
  • 86064-3

ARUP Test Code

0050725

Performing Lab

ARUP Laboratories

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
PNEU PNEUMOCOCCAL AB T1AB Serotype 1 ug/mL 85954-6 86317 x14
PNEU PNEUMOCOCCAL AB T3AB Serotype 3 ug/mL 86080-9  
PNEU PNEUMOCOCCAL AB T4AB Serotype 4 ug/mL 86107-0  
PNEU PNEUMOCOCCAL AB T5AB Serotype 5 ug/mL 86130-2  
PNEU PNEUMOCOCCAL AB T6BAB Serotype 6B ug/mL 27118-9  
PNEU PNEUMOCOCCAL AB T7FAB Serotype 7F ug/mL 25296-5  
PNEU PNEUMOCOCCAL AB T8AB Serotype 8 ug/mL 86147-6  
PNEU PNEUMOCOCCAL AB T9NAB Serotype 9N ug/mL 86169-0  
PNEU PNEUMOCOCCAL AB T9VAB Serotype 9V ug/mL 30153-1  
PNEU PNEUMOCOCCAL AB T12FAB Serotype 12F ug/mL 85977-7  
PNEU PNEUMOCOCCAL AB T14AB Serotype 14 ug/mL 85991-8  
PNEU PNEUMOCOCCAL AB T18CAB Serotype 18C ug/mL 27395-3  
PNEU PNEUMOCOCCAL AB T19FAB Serotype 19F ug/mL 86024-7  
PNEU PNEUMOCOCCAL AB T23FAB Serotype 23F ug/mL 86064-3  
PNEU PNEUMOCOCCAL AB PNINT Interpretation   42771-6  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Collect

Serum separator tube. Postimmunization specimen should be drawn 30 days after immunization and, if shipped separately, must be received within 60 days of preimmunization specimen.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum to an ARUP standard transport tube. (Min: 0.25 mL) MARK SPECIMENS CLEARLY AS "PRE" OR "POST" SO SPECIMENS WILL BE SAVED AND TESTED SIMULTANEOUSLY.

Unacceptable Conditions

Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Storage/Transport Temperature

Refrigerated.

Stability (from collection to initiation)

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 60 days (avoid repeated freeze/thaw cycles)

Performed

Sun-Sat

Referral Lab

Laboratories.
Ordering

Ordering Recommendations

Use to evaluate antibody production and rule out antibody deficiency in patients vaccinated with a pure polysaccharide vaccine (eg, Pneumovax) or protein conjugated vaccine (eg, Prevnar or Vaxneuvance).

Performed

Sun-Sat

Methodology

Quantitative Multiplex Chemiluminescent Immunoassay

Reported

1-3 days

Synonyms

  • Immune Response
  • pneumo conjugate vaccine
  • Pneumo polysaccharide vaccine
  • Strep Pneumo Antibodies
  • Strep Pneumoniae Antibody
  • Strep Vaccine
  • Pneumococcal Antibodies
  • S. Pneumoniae Vaccine
  • Strep Antibodies 14 serotypes
  • Streptococcus pneumoniae Immune Response
  • Streptococcus pneumoniae Vaccine Response
  • Vaccine Response

ARUP Test Code

0050725
Result Interpretation

Interpretive Data

A pre- and postvaccination comparison is required to adequately assess the humoral immune response to the pure polysaccharide Pneumovax 23 (PNX) and/or the protein conjugated Prevnar 7 (P7), Prevnar 13 (P13), Prevnar 20 (P20), and Vaxneuvance (V15) Streptococcus pneumoniae vaccines. Prevaccination samples should be collected prior to vaccine administration. Postvaccination samples should be obtained at least 4 weeks after immunization. Testing of postvaccination samples alone will provide only general immune status of the individual to various pneumococcal serotypes.

In the case of pure polysaccharide vaccine, indication of immune system competence is further delineated as an adequate response to at least 50 percent of the serotypes in the vaccine challenge for those 2-5 years of age and to at least 70 percent of the serotypes in the vaccine challenge for those 6-65 years of age. Individual immune response may vary based on age, past exposure, immunocompetence, and pneumococcal serotype.

Responder Status                    Antibody Ratio

Nonresponder . . . . . . . . . . .  Less than twofold increase and postvaccination concentration less than 1.3 ug/mL

Good responder . . . . . . . . . . .At least a twofold increase and/or a postvaccination concentration greater than or equal to 1.3 ug/mL

A response to 50-70 percent or more of the serotypes in the vaccine challenge is considered a normal humoral response. (Daly, 2014) Antibody concentration greater than 1.0-1.3 ug/mL is generally considered long-term protection. (Daly, 2015)

References:

1. Daly TM, Pickering JW, Zhang X, et al. Multilaboratory assessment of threshold versus fold-change algorithms for minimizing analytical variability in multiplexed pneumococcal IgG measurements. Clin Vaccine Immunol. 2014;21(7):982-988.

2. Daly TM, Hill HR. Use and clinical interpretation of pneumococcal antibody measurements in the evaluation of humoral immune function. Clin Vaccine Immunol. 2015;22(2):148-152.

Administrative

CPT Codes

86317 x14

LOINC

  • 85954-6
  • 86107-0
  • 86130-2
  • 27118-9
  • 86080-9
  • 25296-5
  • 86169-0
  • 85991-8
  • 42771-6
  • 86147-6
  • 30153-1
  • 85977-7
  • 27395-3
  • 86024-7
  • 86064-3

ARUP Test Code

0050725

Performing Lab

ARUP Laboratories
RPS Interface Information

Test Build Information

OrderCode OrderName ResultCode ResultName ResultUnits RESULT LOINC CPT
PNEU PNEUMOCOCCAL AB T1AB Serotype 1 ug/mL 85954-6 86317 x14
PNEU PNEUMOCOCCAL AB T3AB Serotype 3 ug/mL 86080-9  
PNEU PNEUMOCOCCAL AB T4AB Serotype 4 ug/mL 86107-0  
PNEU PNEUMOCOCCAL AB T5AB Serotype 5 ug/mL 86130-2  
PNEU PNEUMOCOCCAL AB T6BAB Serotype 6B ug/mL 27118-9  
PNEU PNEUMOCOCCAL AB T7FAB Serotype 7F ug/mL 25296-5  
PNEU PNEUMOCOCCAL AB T8AB Serotype 8 ug/mL 86147-6  
PNEU PNEUMOCOCCAL AB T9NAB Serotype 9N ug/mL 86169-0  
PNEU PNEUMOCOCCAL AB T9VAB Serotype 9V ug/mL 30153-1  
PNEU PNEUMOCOCCAL AB T12FAB Serotype 12F ug/mL 85977-7  
PNEU PNEUMOCOCCAL AB T14AB Serotype 14 ug/mL 85991-8  
PNEU PNEUMOCOCCAL AB T18CAB Serotype 18C ug/mL 27395-3  
PNEU PNEUMOCOCCAL AB T19FAB Serotype 19F ug/mL 86024-7  
PNEU PNEUMOCOCCAL AB T23FAB Serotype 23F ug/mL 86064-3  
PNEU PNEUMOCOCCAL AB PNINT Interpretation   42771-6  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu

ARUP Consult®

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