Collect

Any site or fluid, urethral swab, endocervical swab, rectal swab or throat swab.

Unacceptable Conditions

Delayed transport of the plates in non-CO2 environment. Refrigerated or frozen specimens

Regional Pathology Services Transport Information

Culture tests:
Test specific transport devices, recommended sources, transport temperature and requirements are required for each test method which include:
Culture, GC Screen vaginal, urethra, rectal, throat, any source.
1.) For culture of N. gonorrhoeae, use rayon or dacron swabs for specimen collection. Cotton fibers contain fatty acids which are inhibitory to the gonococcus. Avoid swabs with wooden sticks.
2.) Inoculate culture directly onto a Jembec agar plate. Direct plating is necessary because the gonococcus is extremely susceptible to drying. Use one plate for each culture obtained. Roll the swab in a Z pattern across the agar surface.
3.) Label the plate with patient identification and specimen site.
4.) Place a CO2 generating tablet in the designated location in the Jembec plate and place in a plastic bag. Seal the bag.
5.) Transport immediately at ambient temperature. If there is any delay in transport, incubate at 37°C.
6.) For male patients, also submit a slide of urethral material for Gram stain. Gram stains of specimens from the endocervix are not as reliable. Stool is not an acceptable specimen for gonorrheal culture.
7.) If disseminated gonococcal infection is suspected, culture blood and suspicious sites such as petechiae or joint fluid.

Storage/Transport Temperature

Inoculate Jembec plate of Modified Thayer Martin agar sealed in a plastic bag with a CO2 generating tablet. Transport immediately at room temperature. If there is any delay in transport, incubate at 37C.

Performed

Microbiology.
Daily.

Remarks

Culture for N. gonorrhoeae is the method of choice in cases of treatment failure and sexual abuse and for non-genital sources. Media and probes are available from laboratory or Regional Pathology Services.

Notes

Identification by nucleic acid methodology can be performed on the genital specimen collected in the GenProbe collection kit. See Chlamydia/GC DNA probe. Note specific site on specimen and test request form.

Performed

Microbiology.
Daily.

Methodology

Standard reference procedure for Neisseria gonorrhoeae culture and identification.

Reported

Preliminary report as soon as found positive, final for negative results at 3 days.

Synonyms

  • GC (Gonorrhoeae) Culture

Reference Interval

Negative for Neisseria gonorrhoeae.

CPT Codes

87081

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
GCSCR GC SCREEN SDES Specimen Source   31208-2 87081
GCSCR GC SCREEN SREQ Additional Info   48767-8  
GCSCR GC SCREEN CULT Culture Result:   41852-5  
GCSCR GC SCREEN RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
GCSCR GC SCREEN SDES Specimen Source Anus ANUS LIST
GCSCR GC SCREEN SDES Specimen Source Cervix CRVX  
GCSCR GC SCREEN SDES Specimen Source Clitoris CLTS  
GCSCR GC SCREEN SDES Specimen Source Foreskin FORS  
GCSCR GC SCREEN SDES Specimen Source Labia LABI  
GCSCR GC SCREEN SDES Specimen Source Labia Left LBLT  
GCSCR GC SCREEN SDES Specimen Source Labia Right LABR  
GCSCR GC SCREEN SDES Specimen Source Miscellaneous Source OTHER  
GCSCR GC SCREEN SDES Specimen Source Penis PENS  
GCSCR GC SCREEN SDES Specimen Source Perineum PRNM  
GCSCR GC SCREEN SDES Specimen Source Rectum RECM  
GCSCR GC SCREEN SDES Specimen Source Scrotum SCRT  
GCSCR GC SCREEN SDES Specimen Source Throat THRT  
GCSCR GC SCREEN SDES Specimen Source Urethra URTH  
GCSCR GC SCREEN SDES Specimen Source Vagina VAGN  
GCSCR GC SCREEN SDES Specimen Source Vaginal VG  
GCSCR GC SCREEN SDES Specimen Source Vaginal/Rectal VR  
GCSCR GC SCREEN SDES Specimen Source Vulva VULV  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 
Collection

Collect

Any site or fluid, urethral swab, endocervical swab, rectal swab or throat swab.

Unacceptable Conditions

Delayed transport of the plates in non-CO2 environment. Refrigerated or frozen specimens

Regional Pathology Services Transport Information

Culture tests:
Test specific transport devices, recommended sources, transport temperature and requirements are required for each test method which include:
Culture, GC Screen vaginal, urethra, rectal, throat, any source.
1.) For culture of N. gonorrhoeae, use rayon or dacron swabs for specimen collection. Cotton fibers contain fatty acids which are inhibitory to the gonococcus. Avoid swabs with wooden sticks.
2.) Inoculate culture directly onto a Jembec agar plate. Direct plating is necessary because the gonococcus is extremely susceptible to drying. Use one plate for each culture obtained. Roll the swab in a Z pattern across the agar surface.
3.) Label the plate with patient identification and specimen site.
4.) Place a CO2 generating tablet in the designated location in the Jembec plate and place in a plastic bag. Seal the bag.
5.) Transport immediately at ambient temperature. If there is any delay in transport, incubate at 37°C.
6.) For male patients, also submit a slide of urethral material for Gram stain. Gram stains of specimens from the endocervix are not as reliable. Stool is not an acceptable specimen for gonorrheal culture.
7.) If disseminated gonococcal infection is suspected, culture blood and suspicious sites such as petechiae or joint fluid.

Storage/Transport Temperature

Inoculate Jembec plate of Modified Thayer Martin agar sealed in a plastic bag with a CO2 generating tablet. Transport immediately at room temperature. If there is any delay in transport, incubate at 37C.

Performed

Microbiology.
Daily.

Remarks

Culture for N. gonorrhoeae is the method of choice in cases of treatment failure and sexual abuse and for non-genital sources. Media and probes are available from laboratory or Regional Pathology Services.

Notes

Identification by nucleic acid methodology can be performed on the genital specimen collected in the GenProbe collection kit. See Chlamydia/GC DNA probe. Note specific site on specimen and test request form.
Ordering

Performed

Microbiology.
Daily.

Methodology

Standard reference procedure for Neisseria gonorrhoeae culture and identification.

Reported

Preliminary report as soon as found positive, final for negative results at 3 days.

Synonyms

  • GC (Gonorrhoeae) Culture
Result Interpretation

Reference Interval

Negative for Neisseria gonorrhoeae.
Administrative

CPT Codes

87081
RPS Interface Information

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
GCSCR GC SCREEN SDES Specimen Source   31208-2 87081
GCSCR GC SCREEN SREQ Additional Info   48767-8  
GCSCR GC SCREEN CULT Culture Result:   41852-5  
GCSCR GC SCREEN RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
GCSCR GC SCREEN SDES Specimen Source Anus ANUS LIST
GCSCR GC SCREEN SDES Specimen Source Cervix CRVX  
GCSCR GC SCREEN SDES Specimen Source Clitoris CLTS  
GCSCR GC SCREEN SDES Specimen Source Foreskin FORS  
GCSCR GC SCREEN SDES Specimen Source Labia LABI  
GCSCR GC SCREEN SDES Specimen Source Labia Left LBLT  
GCSCR GC SCREEN SDES Specimen Source Labia Right LABR  
GCSCR GC SCREEN SDES Specimen Source Miscellaneous Source OTHER  
GCSCR GC SCREEN SDES Specimen Source Penis PENS  
GCSCR GC SCREEN SDES Specimen Source Perineum PRNM  
GCSCR GC SCREEN SDES Specimen Source Rectum RECM  
GCSCR GC SCREEN SDES Specimen Source Scrotum SCRT  
GCSCR GC SCREEN SDES Specimen Source Throat THRT  
GCSCR GC SCREEN SDES Specimen Source Urethra URTH  
GCSCR GC SCREEN SDES Specimen Source Vagina VAGN  
GCSCR GC SCREEN SDES Specimen Source Vaginal VG  
GCSCR GC SCREEN SDES Specimen Source Vaginal/Rectal VR  
GCSCR GC SCREEN SDES Specimen Source Vulva VULV  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 

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