Sputum: early morning collection in a sterile leak-proof container. Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen. Early morning sputum specimen is preferred. Approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations.
Early morning urine in a sterile leak-proof container. Gastric aspirate, body fluid and tissue in a sterile leak-proof container. Store and transport refrigerated at 2-8 C.
5-20 ml sputum, at least 1-2 ml CSF, 5-20 ml gastric aspirate, >= 40ml urine, 5-50 ml body fluid, or 1 gram of tissue.
Unacceptable Conditions
Multiple same-site specimens (more than one in 24 hours; exception sputum (see collection instructions)), dry material, non-sterile or leaking container, improper volume of sputum (<2 ml) or fluids (<1 ml), material collected and transported on a swab and stool.
Storage/Transport Temperature
Refrigerate.
Performed
Microbiology. Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.
Remarks
Collection of early morning specimens of urine and sputum on each of 3 consecutive days is optimum. Gastric lavage specimens must be neutralized with sodium bicarbonate if transport delayed > 4 hours. Collect gastric aspirate 8 hours after food ingestion. Suggest collection of gastric aspirates for 3 consecutive days. Indicate source of specimen on the lab form. Tissue: submit skin lesions or other tissue; keep moistened with sterile saline. Indicate if Mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Client is notified of positive stain/culture. Additional information to include when submitting a specimen for culture; patient's PPD status; BCG immunization history; prior anti-tuberculous therapy.
Please note on test request form if sputum from a Cystic Fibrosis patient.
Notes
DNA probes available for M. tuberculosis complex, M. avium-intracellulare complex, M. gordonae as indicated.
Performed
Microbiology. Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.
Methodology
Standard reference procedures for stain and culture.
Synonyms
AFB Culture w/smear
Reference Interval
Preliminary: Acid fast stain at 24 hours, Negative at 4 weeks
Final: Negative at 6 weeks
Positive cultures are reported as soon as detected.
Note: Identification performed on positives.
Susceptibility performed on Mycobacterium other than M. tuberculosis complex isolates by request.
Susceptibility testing of M. gordonae from sputum is usually inappropriate.
CPT Codes
87116, 87206
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode
OrderName
ResultCode
ResultName
ResultUnits
LOINC
CPT
AFBCU
AFB CULT & SMEAR
SDES
Specimen Source
31208-2
87116, 87206
AFBCU
AFB CULT & SMEAR
SREQ
Additional Info
48767-8
AFBCU
AFB CULT & SMEAR
AFBS
AFB Smear
11545-1
AFBCU
AFB CULT & SMEAR
CULT
Culture Result:
41852-5
AFBCU
AFB CULT & SMEAR
RPT
Report Status
AOE Information (Ask at Order Entry Questions)
Order Code
Order Description
AOE Code
AOE Name
Answer
Answer code
question type
AFBCU
AFB CULT & SMEAR
SDES
Specimen Source
See Full list in Test Directory under Sources for RPS Clients.
Sputum: early morning collection in a sterile leak-proof container. Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen. Early morning sputum specimen is preferred. Approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations.
Early morning urine in a sterile leak-proof container. Gastric aspirate, body fluid and tissue in a sterile leak-proof container. Store and transport refrigerated at 2-8 C.
5-20 ml sputum, at least 1-2 ml CSF, 5-20 ml gastric aspirate, >= 40ml urine, 5-50 ml body fluid, or 1 gram of tissue.
Unacceptable Conditions
Multiple same-site specimens (more than one in 24 hours; exception sputum (see collection instructions)), dry material, non-sterile or leaking container, improper volume of sputum (<2 ml) or fluids (<1 ml), material collected and transported on a swab and stool.
Storage/Transport Temperature
Refrigerate.
Performed
Microbiology. Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.
Remarks
Collection of early morning specimens of urine and sputum on each of 3 consecutive days is optimum. Gastric lavage specimens must be neutralized with sodium bicarbonate if transport delayed > 4 hours. Collect gastric aspirate 8 hours after food ingestion. Suggest collection of gastric aspirates for 3 consecutive days. Indicate source of specimen on the lab form. Tissue: submit skin lesions or other tissue; keep moistened with sterile saline. Indicate if Mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Client is notified of positive stain/culture. Additional information to include when submitting a specimen for culture; patient's PPD status; BCG immunization history; prior anti-tuberculous therapy.
Please note on test request form if sputum from a Cystic Fibrosis patient.
Notes
DNA probes available for M. tuberculosis complex, M. avium-intracellulare complex, M. gordonae as indicated.
Ordering
Performed
Microbiology. Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.
Methodology
Standard reference procedures for stain and culture.
Synonyms
AFB Culture w/smear
Result Interpretation
Reference Interval
Preliminary: Acid fast stain at 24 hours, Negative at 4 weeks
Final: Negative at 6 weeks
Positive cultures are reported as soon as detected.
Note: Identification performed on positives.
Susceptibility performed on Mycobacterium other than M. tuberculosis complex isolates by request.
Susceptibility testing of M. gordonae from sputum is usually inappropriate.
Administrative
CPT Codes
87116, 87206
RPS Interface Information
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
OrderCode
OrderName
ResultCode
ResultName
ResultUnits
LOINC
CPT
AFBCU
AFB CULT & SMEAR
SDES
Specimen Source
31208-2
87116, 87206
AFBCU
AFB CULT & SMEAR
SREQ
Additional Info
48767-8
AFBCU
AFB CULT & SMEAR
AFBS
AFB Smear
11545-1
AFBCU
AFB CULT & SMEAR
CULT
Culture Result:
41852-5
AFBCU
AFB CULT & SMEAR
RPT
Report Status
AOE Information (Ask at Order Entry Questions)
Order Code
Order Description
AOE Code
AOE Name
Answer
Answer code
question type
AFBCU
AFB CULT & SMEAR
SDES
Specimen Source
See Full list in Test Directory under Sources for RPS Clients.