Collect

Sputum:  early morning collection in a sterile leak-proof container.  Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen.  Early morning sputum specimen is preferred. Approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations.

Early morning urine in a sterile leak-proof container. Gastric aspirate, body fluid and tissue in a sterile leak-proof container. Store and transport refrigerated at 2-8 C.

5-20 ml sputum, at  least 1-2 ml CSF, 5-20 ml gastric aspirate, >= 40ml urine, 5-50 ml body fluid, or 1 gram of tissue.
 

Unacceptable Conditions

Multiple same-site specimens (more than one in 24 hours; exception sputum (see collection instructions)), dry material, non-sterile or leaking container, improper volume of sputum (<2 ml) or fluids (<1 ml), material collected and transported on a swab and stool.

Storage/Transport Temperature

Refrigerate.

Performed

Microbiology.
Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.

Remarks

Collection of early morning specimens of urine and sputum on each of 3 consecutive days is optimum. Gastric lavage specimens must be neutralized with sodium bicarbonate if transport delayed > 4 hours. Collect gastric aspirate 8 hours after food ingestion. Suggest collection of gastric aspirates for 3 consecutive days. Indicate source of specimen on the lab form. Tissue: submit skin lesions or other tissue; keep moistened with sterile saline. Indicate if Mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Client is notified of positive stain/culture. Additional information to include when submitting a specimen for culture; patient's PPD status; BCG immunization history; prior anti-tuberculous therapy.

Please note on test request form if sputum from a Cystic Fibrosis patient.

Notes

DNA probes available for M. tuberculosis complex, M. avium-intracellulare complex, M. gordonae as indicated.

Performed

Microbiology.
Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.

Methodology

Standard reference procedures for stain and culture.

Synonyms

  • AFB Culture w/smear

Reference Interval

Preliminary: Acid fast stain at 24 hours, Negative at 4 weeks
Final: Negative at 6 weeks
Positive cultures are reported as soon as detected.
Note: Identification performed on positives.
Susceptibility performed on Mycobacterium other than M. tuberculosis complex isolates by request.
Susceptibility testing of M. gordonae from sputum is usually inappropriate.

CPT Codes

87116, 87206

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
 
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
AFBCU AFB CULT & SMEAR SDES Specimen Source   31208-2 87116, 87206
AFBCU AFB CULT & SMEAR SREQ Additional Info   48767-8  
AFBCU AFB CULT & SMEAR AFBS AFB Smear   11545-1  
AFBCU AFB CULT & SMEAR CULT Culture Result:   41852-5  
AFBCU AFB CULT & SMEAR RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
AFBCU AFB CULT & SMEAR SDES Specimen Source See Full list in Test Directory under Sources for RPS Clients.   List

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 
Collection

Collect

Sputum:  early morning collection in a sterile leak-proof container.  Limit the number of samples to 3 specimens collected consecutively in 8- to 24-hour intervals, with at least one being an early morning specimen.  Early morning sputum specimen is preferred. Approval is required for additional specimens. The number of specimens that should be submitted is related to the results of early smear examinations.

Early morning urine in a sterile leak-proof container. Gastric aspirate, body fluid and tissue in a sterile leak-proof container. Store and transport refrigerated at 2-8 C.

5-20 ml sputum, at  least 1-2 ml CSF, 5-20 ml gastric aspirate, >= 40ml urine, 5-50 ml body fluid, or 1 gram of tissue.
 

Unacceptable Conditions

Multiple same-site specimens (more than one in 24 hours; exception sputum (see collection instructions)), dry material, non-sterile or leaking container, improper volume of sputum (<2 ml) or fluids (<1 ml), material collected and transported on a swab and stool.

Storage/Transport Temperature

Refrigerate.

Performed

Microbiology.
Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.

Remarks

Collection of early morning specimens of urine and sputum on each of 3 consecutive days is optimum. Gastric lavage specimens must be neutralized with sodium bicarbonate if transport delayed > 4 hours. Collect gastric aspirate 8 hours after food ingestion. Suggest collection of gastric aspirates for 3 consecutive days. Indicate source of specimen on the lab form. Tissue: submit skin lesions or other tissue; keep moistened with sterile saline. Indicate if Mycobacteria other than tuberculosis (MOTT) are suspected. This may expedite identification. Client is notified of positive stain/culture. Additional information to include when submitting a specimen for culture; patient's PPD status; BCG immunization history; prior anti-tuberculous therapy.

Please note on test request form if sputum from a Cystic Fibrosis patient.

Notes

DNA probes available for M. tuberculosis complex, M. avium-intracellulare complex, M. gordonae as indicated.

Ordering

Performed

Microbiology.
Monday - Friday by 0800. Stat requests are performed by approval of a pathologist.

Methodology

Standard reference procedures for stain and culture.

Synonyms

  • AFB Culture w/smear
Result Interpretation

Reference Interval

Preliminary: Acid fast stain at 24 hours, Negative at 4 weeks
Final: Negative at 6 weeks
Positive cultures are reported as soon as detected.
Note: Identification performed on positives.
Susceptibility performed on Mycobacterium other than M. tuberculosis complex isolates by request.
Susceptibility testing of M. gordonae from sputum is usually inappropriate.
Administrative

CPT Codes

87116, 87206
RPS Interface Information

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting.
 
OrderCode OrderName ResultCode ResultName ResultUnits LOINC CPT
AFBCU AFB CULT & SMEAR SDES Specimen Source   31208-2 87116, 87206
AFBCU AFB CULT & SMEAR SREQ Additional Info   48767-8  
AFBCU AFB CULT & SMEAR AFBS AFB Smear   11545-1  
AFBCU AFB CULT & SMEAR CULT Culture Result:   41852-5  
AFBCU AFB CULT & SMEAR RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
AFBCU AFB CULT & SMEAR SDES Specimen Source See Full list in Test Directory under Sources for RPS Clients.   List

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
 

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