Collect: Blood or Bone Marrow into a Bactec Mycolytic/F bottle (red/white label). Draw aseptically per blood culture collection procedure. Wipe off top of blood culture bottle with alcohol wipe and let dry. Transport to lab immediately at 20-25 degrees centigrade.
Volume recommendations:
3.0-5.0 mL blood for adults
1.0-3.0 mL blood for pediatrics
1.0 mL minimum volume for bone marrow
Notes: Recommended sample if bone marrow not inoculated into the Bactec Mycolytic/F bottle for submission is SPS tube. Deliver to Microbiology within four hours of collection. Sodium heparin also acceptable.
Blood: EDTA/Lavender tubes, heparin tubes or coagulated blood, Bactec bottle refrigerated or placed in an incubator.
Bone Marrow: EDTA/Lavender tubes, coagulated bone marrow, Bactec bottle refrigerated or placed in an incubator.
Storage/Transport Temperature
Critical ambient storage. Transport to lab ASAP.
Performed
Microbiology. Daily.
Remarks
No smear performed. Comment on lab request form if particular organism suspected. One AFB culture order is recommended per 24 hour period. Bactec Mycolytic/F bottle (red label) is available upon request.
Performed
Microbiology. Daily.
Methodology
Culture
Reported
Negative culture results available at 1 week and final negative at 6 weeks; Positive results will be reported as soon as detected.
Synonyms
AFB Blood Culture
Reference Interval
No Mycobacterium isolated at 6 weeks.
CPT Codes
87116
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting
Collect: Blood or Bone Marrow into a Bactec Mycolytic/F bottle (red/white label). Draw aseptically per blood culture collection procedure. Wipe off top of blood culture bottle with alcohol wipe and let dry. Transport to lab immediately at 20-25 degrees centigrade.
Volume recommendations:
3.0-5.0 mL blood for adults
1.0-3.0 mL blood for pediatrics
1.0 mL minimum volume for bone marrow
Notes: Recommended sample if bone marrow not inoculated into the Bactec Mycolytic/F bottle for submission is SPS tube. Deliver to Microbiology within four hours of collection. Sodium heparin also acceptable.
Blood: EDTA/Lavender tubes, heparin tubes or coagulated blood, Bactec bottle refrigerated or placed in an incubator.
Bone Marrow: EDTA/Lavender tubes, coagulated bone marrow, Bactec bottle refrigerated or placed in an incubator.
Storage/Transport Temperature
Critical ambient storage. Transport to lab ASAP.
Performed
Microbiology. Daily.
Remarks
No smear performed. Comment on lab request form if particular organism suspected. One AFB culture order is recommended per 24 hour period. Bactec Mycolytic/F bottle (red label) is available upon request.
Ordering
Performed
Microbiology. Daily.
Methodology
Culture
Reported
Negative culture results available at 1 week and final negative at 6 weeks; Positive results will be reported as soon as detected.
Synonyms
AFB Blood Culture
Result Interpretation
Reference Interval
No Mycobacterium isolated at 6 weeks.
Administrative
CPT Codes
87116
RPS Interface Information
Test Build Information
Below is the standard RPS build for microbiology cultures. EHR_EMR formatting can be different for some vendors. Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting
