Collect

Collect: Blood or Bone Marrow into a Bactec Mycolytic/F bottle (red/white label).  Draw aseptically per blood culture collection procedure. Wipe off top of blood culture bottle with alcohol wipe and let dry. Transport to lab immediately at 20-25 degrees centigrade.

Volume recommendations: 
  • 3.0-5.0 mL blood for adults
  • 1.0-3.0 mL blood for pediatrics
  • 1.0 mL minimum volume for bone marrow

Notes:  Recommended sample if bone marrow not inoculated into the Bactec Mycolytic/F bottle for submission is SPS tube.  Deliver to Microbiology within four hours of collection.  Sodium heparin also acceptable.

Blood Culture Collection Policy/Procedure

Unacceptable Conditions

Blood:  EDTA/Lavender tubes, heparin tubes or coagulated blood, Bactec bottle refrigerated or placed in an incubator.

Bone Marrow:  EDTA/Lavender tubes, coagulated bone marrow, Bactec bottle refrigerated or placed in an incubator.

Storage/Transport Temperature

Critical ambient storage. Transport to lab ASAP.

Performed

Microbiology.
Daily.

Remarks

No smear performed. Comment on lab request form if particular organism suspected. One AFB culture order is recommended per 24 hour period. Bactec Mycolytic/F bottle (red label) is available upon request.

Performed

Microbiology.
Daily.

Methodology

Culture

Reported

Negative culture results available at 1 week and final negative at 6 weeks; Positive results will be reported as soon as detected.

Synonyms

  • AFB Blood Culture

Reference Interval

No Mycobacterium isolated at 6 weeks.

CPT Codes

87116

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting

 
Order Code OrderName ResultCode ResultName ResultUnits LOINC CPT
AFBBL AFB BLOOD CULTURE SDES Specimen Source   31208-2 87116
AFBBL AFB BLOOD CULTURE SREQ Additional Info   48767-8  
AFBBL AFB BLOOD CULTURE CULT Culture Result:   41852-5  
AFBBL AFB BLOOD CULTURE RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
AFBBL AFB BLOOD CULTURE SDES Specimen Source Blood   BLUD List
AFBBL AFB BLOOD CULTURE     Blood Arterial   BLAR  
AFBBL AFB BLOOD CULTURE     Blood Capillary   BLCP  
AFBBL AFB BLOOD CULTURE     Blood Venous   BLDV  
AFBBL AFB BLOOD CULTURE     Blood, Line Draw   BLLN  
AFBBL AFB BLOOD CULTURE     Blood, Peripheral Draw   BLPH  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu
Collection

Collect

Collect: Blood or Bone Marrow into a Bactec Mycolytic/F bottle (red/white label).  Draw aseptically per blood culture collection procedure. Wipe off top of blood culture bottle with alcohol wipe and let dry. Transport to lab immediately at 20-25 degrees centigrade.

Volume recommendations: 
  • 3.0-5.0 mL blood for adults
  • 1.0-3.0 mL blood for pediatrics
  • 1.0 mL minimum volume for bone marrow

Notes:  Recommended sample if bone marrow not inoculated into the Bactec Mycolytic/F bottle for submission is SPS tube.  Deliver to Microbiology within four hours of collection.  Sodium heparin also acceptable.

Blood Culture Collection Policy/Procedure

Unacceptable Conditions

Blood:  EDTA/Lavender tubes, heparin tubes or coagulated blood, Bactec bottle refrigerated or placed in an incubator.

Bone Marrow:  EDTA/Lavender tubes, coagulated bone marrow, Bactec bottle refrigerated or placed in an incubator.

Storage/Transport Temperature

Critical ambient storage. Transport to lab ASAP.

Performed

Microbiology.
Daily.

Remarks

No smear performed. Comment on lab request form if particular organism suspected. One AFB culture order is recommended per 24 hour period. Bactec Mycolytic/F bottle (red label) is available upon request.
Ordering

Performed

Microbiology.
Daily.

Methodology

Culture

Reported

Negative culture results available at 1 week and final negative at 6 weeks; Positive results will be reported as soon as detected.

Synonyms

  • AFB Blood Culture
Result Interpretation

Reference Interval

No Mycobacterium isolated at 6 weeks.
Administrative

CPT Codes

87116
RPS Interface Information

Test Build Information

Below is the standard RPS build for microbiology cultures.  EHR_EMR formatting can be different for some vendors.  Please contact RPS Interface Support (RPSInterfaceSupport@unmc.edu) for specific information for the vendor specific formatting

 
Order Code OrderName ResultCode ResultName ResultUnits LOINC CPT
AFBBL AFB BLOOD CULTURE SDES Specimen Source   31208-2 87116
AFBBL AFB BLOOD CULTURE SREQ Additional Info   48767-8  
AFBBL AFB BLOOD CULTURE CULT Culture Result:   41852-5  
AFBBL AFB BLOOD CULTURE RPT Report Status      

AOE Information (Ask at Order Entry Questions)

Order Code Order Description AOE Code AOE Name Answer Answer code question type
AFBBL AFB BLOOD CULTURE SDES Specimen Source Blood   BLUD List
AFBBL AFB BLOOD CULTURE     Blood Arterial   BLAR  
AFBBL AFB BLOOD CULTURE     Blood Capillary   BLCP  
AFBBL AFB BLOOD CULTURE     Blood Venous   BLDV  
AFBBL AFB BLOOD CULTURE     Blood, Line Draw   BLLN  
AFBBL AFB BLOOD CULTURE     Blood, Peripheral Draw   BLPH  

Additional Information

For additional information or questions, contact RPSInterfaceSupport@unmc.edu