| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 1 mL |
| Whole blood | 4 mL Purple tube (EDTA) | 1 mL |
| OCT-embedded tissue | Tissue cassette | |
| OCT-embedded tissue | Cryogenic tube | |
| Paraffin embedded tissue | Paraffin block | |
| Tissue (Fresh) | Tissue culture transport media | |
| Tissue (Fresh) | Sterile container with saline | |
| Tissue (Snap-frozen) | Tissue cassette | |
| Tissue (Snap-frozen) | Cryogenic tube | |
| Tissue scrolls (FFPE) | Sterile container |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 1 mL |
| Whole blood | 4 mL Purple tube (EDTA) | 1 mL |
| OCT-embedded tissue | Tissue cassette | |
| OCT-embedded tissue | Cryogenic tube | |
| Paraffin embedded tissue | Paraffin block | |
| Tissue (Fresh) | Tissue culture transport media | |
| Tissue (Fresh) | Sterile container with saline | |
| Tissue (Snap-frozen) | Tissue cassette | |
| Tissue (Snap-frozen) | Cryogenic tube | |
| Tissue scrolls (FFPE) | Sterile container |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| Outpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| Inpatient Requirements |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 1 mL |
| Whole blood | 4 mL Purple tube (EDTA) | 1 mL |
| OCT-embedded tissue | Tissue cassette | |
| OCT-embedded tissue | Cryogenic tube | |
| Paraffin embedded tissue | Paraffin block | |
| Tissue (Fresh) | Tissue culture transport media | |
| Tissue (Fresh) | Sterile container with saline | |
| Tissue (Snap-frozen) | Tissue cassette | |
| Tissue (Snap-frozen) | Cryogenic tube | |
| Tissue scrolls (FFPE) | Sterile container |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| Overview/Billing |
| Interpretation |
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
| NCH Lab Only |
| Specimen Type | Type of Container | Volume of Specimen | Status |
|---|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| Whole blood | 4 mL Purple tube (EDTA) | 4 mL | Preferred |
| OCT-embedded tissue | Tissue cassette | 0.05 gram tissue | Preferred |
| OCT-embedded tissue | Cryogenic tube | 0.05 gram tissue | Alternate |
| Paraffin embedded tissue | Paraffin block | Preferred | |
| Tissue (Fresh) | Tissue culture transport media | 0.05 grams | Preferred |
| Tissue (Fresh) | Sterile container with saline | 0.05 grams | Alternate |
| Tissue (Snap-frozen) | Tissue cassette | mass 0.05 grams | Preferred |
| Tissue (Snap-frozen) | Cryogenic tube | mass 0.05 grams | Alternate |
| Tissue scrolls (FFPE) | Sterile container | Preferred |
| Specimen Type | Type of Container | Minimum Volume |
|---|---|---|
| Bone marrow | 4 mL Purple tube (EDTA) | 1 mL |
| Whole blood | 4 mL Purple tube (EDTA) | 1 mL |
| OCT-embedded tissue | Tissue cassette | |
| OCT-embedded tissue | Cryogenic tube | |
| Paraffin embedded tissue | Paraffin block | |
| Tissue (Fresh) | Tissue culture transport media | |
| Tissue (Fresh) | Sterile container with saline | |
| Tissue (Snap-frozen) | Tissue cassette | |
| Tissue (Snap-frozen) | Cryogenic tube | |
| Tissue scrolls (FFPE) | Sterile container |
If you are an external healthcare provider with no access to Nationwide Children’s Epic system, submission of a completed Oncology Genetic Test Requisition Form is required. If you are an internal ordering provider with access to Nationwide Children’s Epic system, no requisition form is required; please place the lab order electronically in Epic.
If submitting a tissue scroll specimen, please use this Tissue Scroll Calculator tool to determine the thickness and number of tissue scrolls to send to the laboratory.
SPECIMEN INFORMATION:
Fresh and snap-frozen tissue specimens must contain minimum of 10% tumor. Formalin-fixed paraffin embedded (FFPE) tissue block and FFPE tissue scroll specimens must contain minimum of 25% tumor. Please submit the pathology report from the submitted sample. Internal pathology review will also be performed to determine the tumor content in specimen.
If sending FFPE tissue scrolls, an H&E slide from a consecutive cut is also required. Please contact the laboratory at (614) 722-5321 to request the scroll calculator application to be emailed to you to determine the number of scrolls required.
All specimens must be labeled with a patient name and one other identifier (DOB, MRN, Specimen ID#, etc). Sample acquisition prior to receiving therapy is strongly preferred.
SPECIMEN SHIPPING:
Specimens must be shipped as soon as possible after collection due to time-sensitive specimen stability. Ship all samples via overnight courier. Whenever possible, send specimen immediately after collection. For fresh tumor tissue, ship the sample refrigerated using cold packs. If specimen cannot be sent on the collection date, then snap-freeze the tumor tissue immediately after collection and ship the sample frozen (bury the entire tissue in dry ice). Lab must receive specimen within 48 hours. FFPE tissue and FFPE tissue scrolls should be sent at room temperature via overnight shipping. Saturday deliveries are accepted; please check Saturday Delivery on shipment labels.
This test evaluates for the presence of oncogenic gene fusions in hematologic malignancies. Chromosomal translocations resulting in gene fusions are a hallmark of hematologic neoplasias and can help define the diagnosis and potential treatment options. Identifying a specific oncogenic fusion present in the patient offers an opportunity to treat the patient with inhibitors specific to the oncogenic fusion present in the patient.
This panel provides fusion information for 148 gene fusion partners relevant to lymphoid and myeloid malignancies as well as lymphomas, including gene fusion partners associated with Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia, and some, but not all IGH-CRLF2 gene fusions. Please note, this test is not recommended as a first-line diagnostic assay for lymphomas due to reduced sensitivity for detecting genomic rearrangements with cancer associated immunoglobulin loci (IgG, IgL, IgK). Lymphomas that have tested negative for Ig rearrangements by FISH or other methodologies are acceptable. The fusion partner genes included in this panel are listed below:
AICDA, ASB13, BCL2A1, BCL3, ALK, BCL11B, BATF3, BCL2, ABL1, ABL2, BCL6, BCR, BIRC3, BLNK, BMF, BMP7, CBFB, CCDC50, CCND1, CCND2, CCND3, CD274, CD44, CDC25A, CDK6, CDKN2A, CDKN2B, CEBPA, CEBPD, CEBPE, CEBPG, CHD1, CHIC2, CIITA, CREB3L2, CREBBP, CRLF2, CSF1R, CTLA4, CYB5R2, DEK, DENND3, DNMT3B, DNTT, DUSP22, E2F2, EBF1, EIF4A1, ENTPD1, EPOR, ERG, ETV6, EXOC2, FAM216A, FGFR1, FLT3, FOXP1, FUT8, GLIS2, HOXA10, HOXA9, ID4, IKZF1, IKZF2, IKZF3, IL16, IRF4, IRF8, ITPKB, JAK2, KAT6A, KIAA0101, KLF2, KMT2A, LIMD1, LMO1, LMO2, LRMP, LYL1, LZTS1, MAL, MALT1, MAML3, MECOM, MKL1, MLF1, MLLT10, MLLT4, MME, MUC1, MYBL1, MYC, MYH11, NEK6, NF1, NFKB1, NFKB2, NME1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAICS, PAX5, PBX1, PDCD1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PIM1, PIM2, PML, PPAT, PRDM16, PRKAR2B, PTK2B, PTPN1, PYCR1, RAB29, RAG1, RAG2, RANBP1, RARA, RBM15, ROS1, RUNX1, RUNX1T1, S1PR2, SEMA6A, SERPINA9, SETD2, SH3BP5, SOX11, STIL, STRBP, TCF3, TFG, TLX1, TLX3, TNFRSF13B, TNFSF4, TP63, TYK2, WT1, ZCCHC7
Positive results may be confirmed by RT-PCR with Sanger sequencing.
