Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Outpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Stability

Amniotic fluid: Room temperature 24 hour(s)

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

Lab Area

Institute for Genomic Medicine

Methodology

Cell Culture, Cryopreservation

CPT Codes

88235, 88240

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

Inpatient Submit with Specimen

Genetics Prenatal (MFM) Test Requisition Internal

NCH internal providers, for prenatal samples use the NCH Internal Genetics Prenatal (MFM) Test Requisition (do NOT place Epic orders).

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Amniotic fluid Sterile container 10 mL

Inpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Stability

Amniotic fluid: Room temperature 24 hour(s)

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

CPT Codes

88235, 88240

Lab Area

Institute for Genomic Medicine

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

Estimated Patient Price

< $1,000

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

CPT Codes

88235, 88240

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Methodology

Cell Culture, Cryopreservation

Inpatient Submit with Specimen

Genetics Prenatal (MFM) Test Requisition Internal

NCH internal providers, for prenatal samples use the NCH Internal Genetics Prenatal (MFM) Test Requisition (do NOT place Epic orders).

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Amniotic fluid Sterile container 10 mL

Inpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Outpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

InLab Processing

STAT/TIME SENSITIVE SPECIMEN. CPA needs to order SGENSP in Sunquest for Non-EPIC lab order. Send to Cytogenetics Lab ASAP with all submitted paperwork.

Stability

Amniotic fluid: Room temperature 24 hour(s)

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

Methodology

Cell Culture, Cryopreservation

CPT Codes

88235, 88240

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available
Outpatient Requirements

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Outpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Stability

Amniotic fluid: Room temperature 24 hour(s)

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

Lab Area

Institute for Genomic Medicine

Methodology

Cell Culture, Cryopreservation

CPT Codes

88235, 88240

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test
Inpatient Requirements

Inpatient Submit with Specimen

Genetics Prenatal (MFM) Test Requisition Internal

NCH internal providers, for prenatal samples use the NCH Internal Genetics Prenatal (MFM) Test Requisition (do NOT place Epic orders).

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Amniotic fluid Sterile container 10 mL

Inpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Stability

Amniotic fluid: Room temperature 24 hour(s)

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

CPT Codes

88235, 88240

Lab Area

Institute for Genomic Medicine

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

Estimated Patient Price

< $1,000
Overview/Billing

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

CPT Codes

88235, 88240
Interpretation

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Methodology

Cell Culture, Cryopreservation
NCH Lab Only

Inpatient Submit with Specimen

Genetics Prenatal (MFM) Test Requisition Internal

NCH internal providers, for prenatal samples use the NCH Internal Genetics Prenatal (MFM) Test Requisition (do NOT place Epic orders).

Outpatient Submit with Specimen

Collect

Specimen Type Type of Container Volume of Specimen Status
Amniotic fluid Sterile container 20 mL-30 mL Preferred

Minimum Volume

Specimen Type Type of Container Minimum Volume
Amniotic fluid Sterile container 10 mL

Inpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

Outpatient Specimen Preparation

Amniotic fluid: Transport to laboratory as soon as possible
                        Do not add fixative
                        Do not centrifuge
                        Do not freeze
                        Keep at room temperature

InLab Processing

STAT/TIME SENSITIVE SPECIMEN. CPA needs to order SGENSP in Sunquest for Non-EPIC lab order. Send to Cytogenetics Lab ASAP with all submitted paperwork.

Stability

Amniotic fluid: Room temperature 24 hour(s)

Unacceptable Conditions

Delayed or improper handling, Frozen specimen, Fixed specimen

Days Performed

Monday through Friday

Set Up Schedule

8:00-17:00

Typical Turnaround

2 weeks

Remarks

Submission of a completed Prenatal Genetic Test Requisition Form is required.

Clinical Information

This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g., chromosome analysis or prenatal microarray analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial variant targeted sequencing, DNA isolation and storage, or send-out for molecular genetic/biochemical testing to be done on cultured amniocytes. This test includes cryopreservation of cultured amniocytes. If send-out testing and/or DNA isolation and storage are requested, then additional cell culture charge will apply (CPT code: 88235). If obtaining amniotic fluid is not feasible, then fetal fluid may be submitted; please indicate the source of fetal fluid on the requisition form (e.g., cystic hygroma fluid, ascites fluid, bladder fluid, etc.).

If additional in-house or send-out testing on cultured amniocytes is desired, please clearly indicate the desired testing on the requisition form and call the Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate. Otherwise, cultured cells will be cryopreserved for future testing. If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study.

Synonyms

  • Amnio culture only, Amniocyte culture & cryopreservation, Amniocyte cryopreservation for future testing, Amnio culture for send-out testing only, Amniotic fluid culture - no chromosome analysis, Fetal fluid culture only, IGM Test

Methodology

Cell Culture, Cryopreservation

CPT Codes

88235, 88240

Estimated Patient Price

< $1,000

DC Code

5321

Downtime Availability

4-Not available

Lab Area

Lab Area
Institute for Genomic Medicine