Special Instructions

NOTE MINIMUM VOLUME REQUIREMENTS

Ordering Recommendations

This test aids in diagnosis and monitoring of hematopoietic neoplasms. If uncertain whether this test is indicated on blood, CSF or body fluid, consider ordering Pathology Review (PRPBS, PRPCSF, PRPBF) and Flow Cytometry Specimen Hold (FLOWSP), instead. This test is not recommended to evaluate isolated anemia, neutrophilia, basophilia, polycythemia, thrombocytosis or Hodgkin lymphoma.

Test setup varies according to the indication for order, and thus indication is required. See table below for indication. For setup, see Additional Information tab, Technical Information field. To discuss panel setup prior to order, contact Flow Cytometry (801)507-2229. If indication is not provided, test may be delayed or canceled. If test is ordered on multiple concurrent specimens of same type (e.g., bilateral bone marrow aspirates), specimens will be combined.

Collect

Bone marrow:
   2-3 mL (minimum 1 mL if limited sample) aspirate in Dark Green (Sodium Heparin) or Lavender (K2  EDTA)
   MRD study: 3-5 mL from first aspirate pull (label tube #1)
   If dry tap, extra core in RPMI
Peripheral blood:
   3-5 mL in Dark Green (Sodium Heparin) or Lavender (K2 EDTA)
   MRD study: 5 mL in Dark Green (Sodium Heparin) or Lavender (K2 EDTA)
Body fluid: 1-2mL in Dark Green (Sodium Heparin), Lavender (K2 EDTA), or sterile container
Breast periprosthetic implant fluid: >10 mL in sterile container
Cerebrospinal fluid: 1-2mL. Transport in equal volume RPMI. Submit 3rd or 4th tube to minimize bloody tap    RBC contamination. Cell count with differential required. WBC must be ≥ 2K/µL. If <2K/µL, sample will be        considered QNS unless approved by hematopathologist
Tissue: 10mm core or 5mm3 in RPMI (preferred) or saline

Storage/Transport Temperature

Room temperature
or
Refrigerated

Stability (from collection to initiation)

Blood: less than 24 hours preferred (up to 48 hours accepted)
BMA, tissue, CSF or body fluid: less than 24 hours preferred

Unacceptable Conditions

Green PST (Lithium Heparin),
CytoLyt fixative,
Formalin fixative,
Frozen

Performed

Sun-Sat

Reported

Within 2 business days
Some results may be delayed if additional testing is required, for example, special stains, molecular diagnostics, etc.

Methodology

Flow Cytometry

Lab Department

Flow Cytometry

Testing Location

Intermountain

Synonyms

  • Leukemia
  • Flow Cytometry
  • Flow
  • Lymphoma
  • Myeloma

Technical Information

CPT Codes

88184, 88185 each additional marker, 88187, 88188 or 88189

Updated

8/23/24

Interface Order Code (OBR)

FLOWL

Interface Result Code (OBX)

FLOWL
Collecting

Special Instructions

NOTE MINIMUM VOLUME REQUIREMENTS

Ordering Recommendations

This test aids in diagnosis and monitoring of hematopoietic neoplasms. If uncertain whether this test is indicated on blood, CSF or body fluid, consider ordering Pathology Review (PRPBS, PRPCSF, PRPBF) and Flow Cytometry Specimen Hold (FLOWSP), instead. This test is not recommended to evaluate isolated anemia, neutrophilia, basophilia, polycythemia, thrombocytosis or Hodgkin lymphoma.

Test setup varies according to the indication for order, and thus indication is required. See table below for indication. For setup, see Additional Information tab, Technical Information field. To discuss panel setup prior to order, contact Flow Cytometry (801)507-2229. If indication is not provided, test may be delayed or canceled. If test is ordered on multiple concurrent specimens of same type (e.g., bilateral bone marrow aspirates), specimens will be combined.

Collect

Bone marrow:
   2-3 mL (minimum 1 mL if limited sample) aspirate in Dark Green (Sodium Heparin) or Lavender (K2  EDTA)
   MRD study: 3-5 mL from first aspirate pull (label tube #1)
   If dry tap, extra core in RPMI
Peripheral blood:
   3-5 mL in Dark Green (Sodium Heparin) or Lavender (K2 EDTA)
   MRD study: 5 mL in Dark Green (Sodium Heparin) or Lavender (K2 EDTA)
Body fluid: 1-2mL in Dark Green (Sodium Heparin), Lavender (K2 EDTA), or sterile container
Breast periprosthetic implant fluid: >10 mL in sterile container
Cerebrospinal fluid: 1-2mL. Transport in equal volume RPMI. Submit 3rd or 4th tube to minimize bloody tap    RBC contamination. Cell count with differential required. WBC must be ≥ 2K/µL. If <2K/µL, sample will be        considered QNS unless approved by hematopathologist
Tissue: 10mm core or 5mm3 in RPMI (preferred) or saline

Storage/Transport Temperature

Room temperature
or
Refrigerated

Stability (from collection to initiation)

Blood: less than 24 hours preferred (up to 48 hours accepted)
BMA, tissue, CSF or body fluid: less than 24 hours preferred

Unacceptable Conditions

Green PST (Lithium Heparin),
CytoLyt fixative,
Formalin fixative,
Frozen
Resulting

Performed

Sun-Sat

Reported

Within 2 business days
Some results may be delayed if additional testing is required, for example, special stains, molecular diagnostics, etc.

Methodology

Flow Cytometry
Additional Information

Lab Department

Flow Cytometry

Testing Location

Intermountain

Synonyms

  • Leukemia
  • Flow Cytometry
  • Flow
  • Lymphoma
  • Myeloma

Technical Information

Coding

CPT Codes

88184, 88185 each additional marker, 88187, 88188 or 88189

Updated

8/23/24
Interface Mapping

Interface Order Code (OBR)

FLOWL

Interface Result Code (OBX)

FLOWL