Sputum, Tracheal Aspirates, Bronchioalveolar lavage (BAL), gastric lavages and sinus aspirates collected in sterile specimen container with tight-fitting lid. Urine - three consecutive first morning specimens preferred.
Blood Culture table information is for abbreviated. For full details, click on the link to CLI https://www.testmenu.com/cincinnatichildrens/Tests/666307
Mycobacterial Culture (AFB, TB)
Green Top 4mL Sodium Heparin
Minimum Collection Volume
Sputum, Tracheal Aspirates and BAL: 1 mL
Gastric Lavage: 1 mL
Urine: 10 mL
Blood/Bone Marrow: 2.0mL
Acceptable Specimen Collect Alternatives
Aspirated pus and/or Body fluids other than CSF: Collect in syringe with luer-lok ®cap.
Stability (from collection to initiation)
Ambient (Green Top 4mL Sodium Heparin)- 1 day
Refrigerated-3 days
Patient Preparation
Recommended collection: Three sputum or gastric lavage specimens at 8-24 hour intervals (24 hours when possible) and at least one first-morning specimen. An individual order must be submitted for each specimen.
Specimen Preparation
Respiratory specimens and Gastric Lavages: Transfer (for each collection) 5-10 mL to a sterile container.
Body Fluids or CSF: Transfer 5 mL to a sterile container.
Tissue: Transfer to a sterile container.
Urine: Transfer at least 40 mL to a sterile container.
Blood/Bone Marrow: Collect 4 mL blood into aGreen Top 4mL Sodium Heparin. Minimum: 2.0 mL. Call Micro 6-8144 for Green Top 4mL Sodium Heparin
Specimen source required. Acid fast stain will not be performed on blood/bone marrow specimens.
Units of Measure
See Reference Interval
Synonyms
Culture, AFB
C AFB
AFB Culture and stain
Mycobacteria Culture
TB Culture
Tests Included
Acid Fast (Mycobacteria sp.) Stain and Culture
Reflex Testing
If positive, identification and sensitivities will follow, as appropriate
Methodology
Stain / Culture / Identification / Susceptibility
Reported
Stain result same day specimen is processed
Preliminary culture report at 24 hours
Final culture report at 6 weeks, If positive, notification will follow
Reference Interval
Culture and stain negative for acid fast bacilli
CPT Codes
87015, 87116, 87206
Lab Use Only
Ambient-Isolator Tube
Refrigerate-All other specimen types collected.
Collection
Collect
Sputum, Tracheal Aspirates, Bronchioalveolar lavage (BAL), gastric lavages and sinus aspirates collected in sterile specimen container with tight-fitting lid. Urine - three consecutive first morning specimens preferred.
Blood Culture table information is for abbreviated. For full details, click on the link to CLI https://www.testmenu.com/cincinnatichildrens/Tests/666307
Mycobacterial Culture (AFB, TB)
Green Top 4mL Sodium Heparin
Minimum Collection Volume
Sputum, Tracheal Aspirates and BAL: 1 mL
Gastric Lavage: 1 mL
Urine: 10 mL
Blood/Bone Marrow: 2.0mL
Acceptable Specimen Collect Alternatives
Aspirated pus and/or Body fluids other than CSF: Collect in syringe with luer-lok ®cap.
Stability (from collection to initiation)
Ambient (Green Top 4mL Sodium Heparin)- 1 day
Refrigerated-3 days
Patient Preparation
Recommended collection: Three sputum or gastric lavage specimens at 8-24 hour intervals (24 hours when possible) and at least one first-morning specimen. An individual order must be submitted for each specimen.
Specimen Preparation
Respiratory specimens and Gastric Lavages: Transfer (for each collection) 5-10 mL to a sterile container.
Body Fluids or CSF: Transfer 5 mL to a sterile container.
Tissue: Transfer to a sterile container.
Urine: Transfer at least 40 mL to a sterile container.
Blood/Bone Marrow: Collect 4 mL blood into aGreen Top 4mL Sodium Heparin. Minimum: 2.0 mL. Call Micro 6-8144 for Green Top 4mL Sodium Heparin
