Blood in 4mL DkGr NaHep. Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 9am
Minimum Collection Volume
2mL
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Sample must be received within 24 hours of collection.
Stability (from collection to initiation)
Room Temp 24 Hours
Notes
Th17 are CD4+ T cells which secrete IL-17. Various diseases show disturbances in the proportion of circulating Th17 cells. Hyper IgE Syndrome shows decreases in Th17 cells. Many inflammatory and autoimmune conditions can show alterations.Th17 cells are identified by observing intracellular IL-17 production. Cytokine production is stimulated by incubating whole blood with Phorbol 12-Myristate 13 Acetate and Ionomycin. Activation is carried out in the presence of Brefeldin A, which inhibits intracellular transport, causing cytokines produced during the activation to be retained inside the cell. The activated cells are stained with surface monoclonal antibodies for phenotyping, fixed, permeabilized and stained with antibody to IL-17A.
Blood in 4mL DkGr NaHep. Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 9am
Minimum Collection Volume
2mL
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Sample must be received within 24 hours of collection.
Stability (from collection to initiation)
Room Temp 24 Hours
Notes
Th17 are CD4+ T cells which secrete IL-17. Various diseases show disturbances in the proportion of circulating Th17 cells. Hyper IgE Syndrome shows decreases in Th17 cells. Many inflammatory and autoimmune conditions can show alterations.Th17 cells are identified by observing intracellular IL-17 production. Cytokine production is stimulated by incubating whole blood with Phorbol 12-Myristate 13 Acetate and Ionomycin. Activation is carried out in the presence of Brefeldin A, which inhibits intracellular transport, causing cytokines produced during the activation to be retained inside the cell. The activated cells are stained with surface monoclonal antibodies for phenotyping, fixed, permeabilized and stained with antibody to IL-17A.
