Blood in 4mL DkGrn NaHep (2 tubes needed). Only collect patient samples M-Th, do NOT collect F-Su. Please call if a holiday falls during the week for increased testing restrictions.
Minimum Collection Volume
Contact Lab for minimum volumes (based on ALC)
Stability (from collection to initiation)
Room Temp 28 Hours
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Sample must be received within 24 hours of collection.
Natural Killer (NK) cells are a subset of cytotoxic lymphocytes that have the ability to induce cell death in tumor cells or in virally infected cells. NK cells play a crucial role in the homeostasis of the immune system. They are typically characterized by the expression of CD16 and CD56 on their cell surface.
This assay makes use of the fact that NK cells will lyse the human erythroleukemia cell line, K562, in vitro. K562 cells are loaded with radioactive chromium and incubated with various ratios of a mononuclear cell preparation. Lysis of the K562 targets by NK cells contained in the cell preparation is measured by the amount of chromium released into the supernatant.
CPT Codes
86352, 86357
LOINC
NK 50:1 82728-7
NK 25:1 82727-9
NK 12.5:1 82726-1
NK 6.25:1 82725-3
NK Lytic Unit 34439-0
NK (CD16/56%) 8112-5
Collection
Notes
This test is New York DOH approved.
Collect
Blood in 4mL DkGrn NaHep (2 tubes needed). Only collect patient samples M-Th, do NOT collect F-Su. Please call if a holiday falls during the week for increased testing restrictions.
Minimum Collection Volume
Contact Lab for minimum volumes (based on ALC)
Stability (from collection to initiation)
Room Temp 28 Hours
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Sample must be received within 24 hours of collection.
Natural Killer (NK) cells are a subset of cytotoxic lymphocytes that have the ability to induce cell death in tumor cells or in virally infected cells. NK cells play a crucial role in the homeostasis of the immune system. They are typically characterized by the expression of CD16 and CD56 on their cell surface.
This assay makes use of the fact that NK cells will lyse the human erythroleukemia cell line, K562, in vitro. K562 cells are loaded with radioactive chromium and incubated with various ratios of a mononuclear cell preparation. Lysis of the K562 targets by NK cells contained in the cell preparation is measured by the amount of chromium released into the supernatant.
