Collect

Blood in 3mL Lav EDTA. Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 1400 

Minimum Collection Volume

0.5mL

Acceptable Specimen Collect Alternatives

4mL DkGr NaHep

Shipping/Handling Instructions (Lab Use Only)

Do not spin. Critical: Room Temp

External Client Shipping and Handling

Sample must be received within 24 hours of collection.

Stability (from collection to initiation)

Room Temp 24 Hours 

Notes

If collecting sample in a Sodium Heparin (NaHep) Tube: A concurrent CBC/Diff should be drawn with this assay or the results of a concurrent CBC/Diff should be sent so that absolute cell values can be reported.

Performing Lab

CBDI DIL:
Phone: 513-636-4685
Fax: 513-636-3861
Email: CBDILabs@cchmc.org

Units of Measure

%, cells/mcL

Synonyms

  • Lymphocyte Subpopulations
  • CD8
  • CD4
  • IMMUNOPHENOTYPING
  • LYMPHOCYTE SUBSET
  • FLOW CYTOMETRY
  • LYMPH SUBSET
  • LYMPH
  • LYMPH SUB
  • LYMPH SUBPOPS
  • LYMPHOCYTE
  • SUBS
  • SUBSET
  • SUBPOP
  • CD19
  • CD56
  • CD16

Tests Included

% and absolute count for the lymphocyte subpopulations: Total T cells (CD3), T helper cells (CD4), T cytotoxic cells (CD8), B cells (CD19), and NK cells (CD16/CD56); CD4:CD8 ratio

Methodology

Flow Cytometry

Reported

2 days

CPT Codes

86360, 86359, 86355, 86357
Collection

Collect

Blood in 3mL Lav EDTA. Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 1400 

Minimum Collection Volume

0.5mL

Acceptable Specimen Collect Alternatives

4mL DkGr NaHep

Shipping/Handling Instructions (Lab Use Only)

Do not spin. Critical: Room Temp

External Client Shipping and Handling

Sample must be received within 24 hours of collection.

Stability (from collection to initiation)

Room Temp 24 Hours 

Notes

If collecting sample in a Sodium Heparin (NaHep) Tube: A concurrent CBC/Diff should be drawn with this assay or the results of a concurrent CBC/Diff should be sent so that absolute cell values can be reported.

Performing Lab

CBDI DIL:
Phone: 513-636-4685
Fax: 513-636-3861
Email: CBDILabs@cchmc.org

Units of Measure

%, cells/mcL
Ordering

Synonyms

  • Lymphocyte Subpopulations
  • CD8
  • CD4
  • IMMUNOPHENOTYPING
  • LYMPHOCYTE SUBSET
  • FLOW CYTOMETRY
  • LYMPH SUBSET
  • LYMPH
  • LYMPH SUB
  • LYMPH SUBPOPS
  • LYMPHOCYTE
  • SUBS
  • SUBSET
  • SUBPOP
  • CD19
  • CD56
  • CD16

Tests Included

% and absolute count for the lymphocyte subpopulations: Total T cells (CD3), T helper cells (CD4), T cytotoxic cells (CD8), B cells (CD19), and NK cells (CD16/CD56); CD4:CD8 ratio

Methodology

Flow Cytometry

Reported

2 days
Result Interpretation
Laboratory Personnel Use

CPT Codes

86360, 86359, 86355, 86357