This procedure is a flow cytometric assay that uses whole blood to screen for abnormalities in the expression of CD40L and binding of CD40-Ig FP (Fusion Protein) on the surface of in vitro activated CD4+ T cells. It has also been observed that both gene and surface expression of CD40L by activated T cells is depressed in a subgroup of common variable immunodeficiency.
This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient’s clinical presentation suggests that the probability of a diagnosis is high.
CPT Codes
88184, 88185 x 5, 88187
Collection
Collect
Blood in 4mL DkGr NaHep. Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 9am
Minimum Collection Volume
2mL
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Room temperature only. Must be received in lab within 24 hours of collection.
This procedure is a flow cytometric assay that uses whole blood to screen for abnormalities in the expression of CD40L and binding of CD40-Ig FP (Fusion Protein) on the surface of in vitro activated CD4+ T cells. It has also been observed that both gene and surface expression of CD40L by activated T cells is depressed in a subgroup of common variable immunodeficiency.
This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient’s clinical presentation suggests that the probability of a diagnosis is high.
