3 mL blood in Dark Green (Sodium Heparin) or
3 mL blood in Red or Gold tubes
Stability (from collection to initiation)
After separation from cells:
Frozen -20°C: 1 week
Frozen -70°C: 1 month
3 mL whole blood for up to 24 hours, if the tube has not been opened and has been refrigerated
Specimen Preparation
CCHMC Outpatient Lab: Transport centrifiged, unopened, refrigerated Lithium Heparin plasma or Sodium Heparin whole blood within 24 hours to CCHMC Processing Department.
Lab Processing Instructions: Blood should be centrifuged and separated from cells immediately after receipt if not already centrifuged. Transfer plasma or serum into a polypropylene tube, then cap and immediately freeze at -80 C.
Blood should be centrifuged and separated from cells immediately. Transfer plasma to polypropylene tube, cap and freeze at -70°C or below.
Outside providers and researchers: Mail frozen on dry ice Monday - Thursday only.
CCHMC processors: Transport to Special Chemistry Lab frozen.
Collection
Collect
3 mL blood in Light Green (Lithium Heparin)
Minimum Collection Volume
1.0 mL Plasma or Serum (Plasma is preferred)
Acceptable Specimen Collect Alternatives
3 mL blood in Dark Green (Sodium Heparin) or
3 mL blood in Red or Gold tubes
Stability (from collection to initiation)
After separation from cells:
Frozen -20°C: 1 week
Frozen -70°C: 1 month
3 mL whole blood for up to 24 hours, if the tube has not been opened and has been refrigerated
Specimen Preparation
CCHMC Outpatient Lab: Transport centrifiged, unopened, refrigerated Lithium Heparin plasma or Sodium Heparin whole blood within 24 hours to CCHMC Processing Department.
Lab Processing Instructions: Blood should be centrifuged and separated from cells immediately after receipt if not already centrifuged. Transfer plasma or serum into a polypropylene tube, then cap and immediately freeze at -80 C.
