Collect

3 mL of whole peripheral blood in a Dark Green Sodium Heparin tube (Na Hep).
- The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

Specify what TYPE of FISH is required on the requisition. If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

1 mL (adult) or 0.5 mL (infant) of whole peripheral blood in a Dark Green Sodium Heparin tube (Na Hep).

If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

ACD (yellow top), Na Heparin (Dark Blue top) and Na Heparin (Tan top) tubes are acceptable.

Patient Preparation

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for constitutional testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

Storage/Transport Temperature

Store at room temperature.

Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Unacceptable Conditions

Lithium Heparin (which also comes in a green top tube) can cause culture failure.

Synonyms

  • 4750200 - FISH : PWS/AS (15Q11-13) SYNDROME
  • 4396818 - FISH : STS (XP22.3) REGION
  • 4399168 - FISH : ANEUPLOIDY 21 (Constitutional)
  • 4750180 - FISH : MILLER-DIEKER (17P13) SYNDROME
  • 4396801 - FISH : SOTOS (5Q31) SYNDROME
  • 4750240 - FISH : KALLMAN (XP22.3) SYNDROME
  • 4750190 - FISH : SMITH-MAGENIS (17P11.2) SYNDROME
  • 4397554 - FISH : XY CONSTITUTIONAL
  • 4750220 - FISH : WILLIAMS (7Q11.23) SYNDROME
  • 4405593 - FISH : CEP 18 (TRISOMY 18)
  • 4750990 - FISH : VCF (22Q11.2) SYNDROME
  • 4750300 - FISH : 1P36 DEL SYND - CONSTITUTIONAL
  • 4397580 - FISH : SRY (YP11.2) REGION
  • 4396787 - FISH : WOLF-HIRSCHHORN (4P16) SYNDROME
  • 4398434 - FISH : RB-1 (13Q14) (Constitutional)
  • 4396794 - FISH : CRI-DU-CHAT (5P15.2) SYNDROME
  • 4750290 - FISH : X-ist for X-inactivation (using FISH : Miscellaneous)
  • 4750290 - FISH : Miscellaneous FISH (Constitutional)
  • 4405619 - FISH : CEP X - centromere
  • 4405627 - FISH : CEP Y (MISC)
  • FISH Probe : Microdeletion (237)
  • FISH Probe : Heritable (237)
  • FISH Probe : Inherited (237)
  • FISH Probe : Germ Line (237)

Tests Included

These FISH (Fluorescent in-situ Hybridization) tests listed on this page are to rule out Constitutional (i.e., heritable; germ-line) FISH abnormalities.  Constitutional abnormalities are those that are found in all or many of the cells in the body and that were present prior to birth.

FISH Probes Performed by the CCHMC Cytogenetics Lab for Constitutional (Heritable) Studies Orderable in EPIC:
Multiple Chromosome:
FISH : CEP Probe (MISC) - multiple different chromosome centromere probes are available
FISH : Miscellaneous FISH
FISH : Subtelomere Probe (MISC)  - multiple different chromosome telomere probes are available
Chromosome 1
FISH : 1p36 Del Synd - Constitutional
Chromosome 4
FISH : Wolf-Hirschhorn (4p16) Syndrome
Chromosome 5
FISH : Cri-Du-Chat (5p15.2) Syndrome
FISH : Sotos (5q31) Syndrome
Chromosome 7
FISH : Williams (7q11.23) Syndrome
Chromosome 13
FISH : RB-1 (13q14)  - used for trisomy 13 and retinoblastoma deletion detection
Chromosome 15
FISH : PWS/AS (15q11-13) Syndrome
Chromosome 17
FISH : Miller-Dieker (17p13) Syndrome
FISH : Smith-Magenis (17p11.2) Syndrome
Chromosome 18
FISH : CEP 18 (Trisomy 18)
Chromosome 20
FISH : JAG1 (20p12)
Chromosome 21
FISH : Aneuploidy 21
Chromosome 22
FISH : VCF (22q11.2) Syndrome
Chromosomes X and Y
FISH : CEP X - centromere
FISH : Kallman (Xp22.3) Syndrome
FISH : STS (Xp22.3) Region
FISH : SHOX (Xp22/Yp1.3) Region
FISH : Miscellaneous for X-ist (X inactivation center)
FISH : CEP Y (MISC) - multiple different chromosome Y probes are available
FISH : SRY (Yp11.2) Region
FISH : XY Constitutional

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

7 Days for routine analysis

2 Days for STAT analysis (specify on requisition if STAT - additional charge will apply)

CPT Codes

88271, 88273, 88274

Add 88230 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.

Lab Use Only

SPECIFY WHAT TYPE OF FISH PROBE / IF ORDERING THIS TEST ALONE (W/O CHROMOSOME STUDY) A CULTURE CHARGE (SPECIMEN DEPENDENT) WILL BE ADDED.
Collection

Collect

3 mL of whole peripheral blood in a Dark Green Sodium Heparin tube (Na Hep).
- The preferred choice for specimen collection is listed above, however, if that tube type/container is not available please call the lab for advice on alternative specimen collection choices (513-636-4474).  If the specimen is already collected in a different tube type, send the sample to the lab and we will make every effort to process and result the test.

Specify what TYPE of FISH is required on the requisition. If the FISH probe test that you require is not listed on the test requisition, please hand-write the name of the test you want to order in the space provided.

Collection personnel MUST initial specimen container to confirm sample identity.

Minimum Collection Volume

1 mL (adult) or 0.5 mL (infant) of whole peripheral blood in a Dark Green Sodium Heparin tube (Na Hep).

If a lesser volume of blood is sent, the laboratory will attempt to perform the test requested.  The laboratory cannot assure either a result and/or the ability to perform repeat testing and/or additional testing if the minimum volumes are not met.

Acceptable Specimen Collect Alternatives

ACD (yellow top), Na Heparin (Dark Blue top) and Na Heparin (Tan top) tubes are acceptable.

Patient Preparation

Please go to "ORDERING" tab to see a complete list of the stand-alone FISH Probes we offer for constitutional testing.  Please use our Test Requisition (see link at bottom of next tab) and fill in the FISH probe you require.  If you do not see a FISH probe that you need in the listing, please call our lab to see if we can order it for your testing.

Specimen Preparation

Label tubes with patient name and date of birth (DOB).

Storage/Transport Temperature

Store at room temperature.

Use overnight shipping (protect from temperature extremes, no ice) or call the lab for local courier service (contact laboratory prior to drawing specimen to ensure courier area coverage).

Performing Lab

Cytogenetics Laboratory (513) 636-4474  /  FAX: (513) 636-4373

Performed

6 AM - 12 AM (Monday through Friday, with more limited hours on the weekend)

Unacceptable Conditions

Lithium Heparin (which also comes in a green top tube) can cause culture failure.

Ordering

Synonyms

  • 4750200 - FISH : PWS/AS (15Q11-13) SYNDROME
  • 4396818 - FISH : STS (XP22.3) REGION
  • 4399168 - FISH : ANEUPLOIDY 21 (Constitutional)
  • 4750180 - FISH : MILLER-DIEKER (17P13) SYNDROME
  • 4396801 - FISH : SOTOS (5Q31) SYNDROME
  • 4750240 - FISH : KALLMAN (XP22.3) SYNDROME
  • 4750190 - FISH : SMITH-MAGENIS (17P11.2) SYNDROME
  • 4397554 - FISH : XY CONSTITUTIONAL
  • 4750220 - FISH : WILLIAMS (7Q11.23) SYNDROME
  • 4405593 - FISH : CEP 18 (TRISOMY 18)
  • 4750990 - FISH : VCF (22Q11.2) SYNDROME
  • 4750300 - FISH : 1P36 DEL SYND - CONSTITUTIONAL
  • 4397580 - FISH : SRY (YP11.2) REGION
  • 4396787 - FISH : WOLF-HIRSCHHORN (4P16) SYNDROME
  • 4398434 - FISH : RB-1 (13Q14) (Constitutional)
  • 4396794 - FISH : CRI-DU-CHAT (5P15.2) SYNDROME
  • 4750290 - FISH : X-ist for X-inactivation (using FISH : Miscellaneous)
  • 4750290 - FISH : Miscellaneous FISH (Constitutional)
  • 4405619 - FISH : CEP X - centromere
  • 4405627 - FISH : CEP Y (MISC)
  • FISH Probe : Microdeletion (237)
  • FISH Probe : Heritable (237)
  • FISH Probe : Inherited (237)
  • FISH Probe : Germ Line (237)

Tests Included

These FISH (Fluorescent in-situ Hybridization) tests listed on this page are to rule out Constitutional (i.e., heritable; germ-line) FISH abnormalities.  Constitutional abnormalities are those that are found in all or many of the cells in the body and that were present prior to birth.

FISH Probes Performed by the CCHMC Cytogenetics Lab for Constitutional (Heritable) Studies Orderable in EPIC:
Multiple Chromosome:
FISH : CEP Probe (MISC) - multiple different chromosome centromere probes are available
FISH : Miscellaneous FISH
FISH : Subtelomere Probe (MISC)  - multiple different chromosome telomere probes are available
Chromosome 1
FISH : 1p36 Del Synd - Constitutional
Chromosome 4
FISH : Wolf-Hirschhorn (4p16) Syndrome
Chromosome 5
FISH : Cri-Du-Chat (5p15.2) Syndrome
FISH : Sotos (5q31) Syndrome
Chromosome 7
FISH : Williams (7q11.23) Syndrome
Chromosome 13
FISH : RB-1 (13q14)  - used for trisomy 13 and retinoblastoma deletion detection
Chromosome 15
FISH : PWS/AS (15q11-13) Syndrome
Chromosome 17
FISH : Miller-Dieker (17p13) Syndrome
FISH : Smith-Magenis (17p11.2) Syndrome
Chromosome 18
FISH : CEP 18 (Trisomy 18)
Chromosome 20
FISH : JAG1 (20p12)
Chromosome 21
FISH : Aneuploidy 21
Chromosome 22
FISH : VCF (22q11.2) Syndrome
Chromosomes X and Y
FISH : CEP X - centromere
FISH : Kallman (Xp22.3) Syndrome
FISH : STS (Xp22.3) Region
FISH : SHOX (Xp22/Yp1.3) Region
FISH : Miscellaneous for X-ist (X inactivation center)
FISH : CEP Y (MISC) - multiple different chromosome Y probes are available
FISH : SRY (Yp11.2) Region
FISH : XY Constitutional

Methodology

Fluorescence In Situ Hybridization

If multiple single FISH or FISH Panel tests are ordered at the same time, only ONE culture charge is added for the entire group.

Reported

7 Days for routine analysis

2 Days for STAT analysis (specify on requisition if STAT - additional charge will apply)

Result Interpretation
Laboratory Personnel Use

CPT Codes

88271, 88273, 88274

Add 88230 CPT for culture of FISH cells - added once per encounter, if necessary, to obtain cells for FISH analysis. This charge is dependent on what other testing is requested.

Please call 1-866-450-4198 for pricing or with any billing questions.

Lab Use Only

SPECIFY WHAT TYPE OF FISH PROBE / IF ORDERING THIS TEST ALONE (W/O CHROMOSOME STUDY) A CULTURE CHARGE (SPECIMEN DEPENDENT) WILL BE ADDED.