Call Central Labs for special tube collection kit.
Must collect 1mL blood in each of the four specimen tubes. Invert tubes 10 times to ensure inner surface of the tube is completely coated with blood to solubilize antigens on the tube walls.
NOTE: Over-energetic shaking may cause gel disruption and could lead to aberrant results.
Central Labs: DO NOT CENTRIFUGE UPON RECEIPT. All Quantiferon tubes are to be placed into the incubator as a batch at 1800 and removed 16-24 hrs later.
Once removed from incubator, centrifuge for 15 minutes and store tubes in ARUP refrigerator rack to await courier pick-up.
Unacceptable Conditions
Centrifuged samples and samples improperly collected in special tubes. Received >16 hours old. Refrigerated or frozen. Specimens not collected in Quantiferon®-TB Gold Plus collection tubes
Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is less in low-risk populations. Test may be used in persons who have received BCG vaccine.
QuantiFERON-TB Gold In Tube & Mycobacterium tuberculosis Antibody
Reference Interval
Components
Reference Interval
QuantiFERON-TB Gold In-Tube
Negative
QuantiFERON-TB1 minus NIL
0.34 IU/mL or less
QuantiFERON-TB2 minus NIL
0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL
No reference interval
QuantiFERON NIL
No reference interval
Notes
Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.
A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection --- United States, 2010 (), for more information concerning test performance in low-prevalence populations and use in occupational screening.
CPT Codes
86480
LOINC
71773-6
64084-7
71774-4
71776-9
Collection
Collect
Call Central Labs for special tube collection kit.
Must collect 1mL blood in each of the four specimen tubes. Invert tubes 10 times to ensure inner surface of the tube is completely coated with blood to solubilize antigens on the tube walls.
NOTE: Over-energetic shaking may cause gel disruption and could lead to aberrant results.
Central Labs: DO NOT CENTRIFUGE UPON RECEIPT. All Quantiferon tubes are to be placed into the incubator as a batch at 1800 and removed 16-24 hrs later.
Once removed from incubator, centrifuge for 15 minutes and store tubes in ARUP refrigerator rack to await courier pick-up.
Unacceptable Conditions
Centrifuged samples and samples improperly collected in special tubes. Received >16 hours old. Refrigerated or frozen. Specimens not collected in Quantiferon®-TB Gold Plus collection tubes
Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is less in low-risk populations. Test may be used in persons who have received BCG vaccine.
QuantiFERON-TB Gold In Tube & Mycobacterium tuberculosis Antibody
Result Interpretation
Reference Interval
Components
Reference Interval
QuantiFERON-TB Gold In-Tube
Negative
QuantiFERON-TB1 minus NIL
0.34 IU/mL or less
QuantiFERON-TB2 minus NIL
0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL
No reference interval
QuantiFERON NIL
No reference interval
Notes
Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.
A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection --- United States, 2010 (), for more information concerning test performance in low-prevalence populations and use in occupational screening.
