Allow sample to clot fully for 30 minutes at room temperature
Centrifuge within 2 hours of collection
Transfer serum to plastic transport tube.
Samples may be frozen and thawed up to 3 times. Mix frozen samples well after thawing.
Storage/Transport Temperature
Frozen. Submit specimen in plastic transport tube.
Stability (from collection to initiation)
Ambient: 24 hours
Refrigerated: 7 days
Frozen: 1 month
Unacceptable Conditions
Hemolyzed, icteric, lipemic or bacterially contaminated sera.
Performed
Monday - Friday
Reported
Same day
Performing Lab
Avera McKennan Regional Laboratory
CPT Codes
86039
Compliance & Service Center Alerts
ANA Screen Only. Does not include reflex testing. If reflex testing is clinically indicated, order ANA by IFA with Reflex to ENA.
Methodology
Indirect Immunofluorescence using Hep-2 cells
Interpretive Data
ANA is a standard screening assay for detecting multiple autoantibodies produced by patients with an ANA associated rheumatic disease. In 2009 a task force of the American College of Rheumatology issued a statement declaring Hep-2 indirect immunofluorescence as the preferred method for ANA screening.
Avera McKennan Laboratory will report 5 patterns and respective titers: Homogeneous, Speckled, Centromere, Nucleolar and Nuclear Dots. Cytoplasmic staining will be noted if present.
Ordering / Collection
Test Mnemonic
ANA
Collect
Serum; Plain Red or Serum Separator Tube (SST)
Minimum Volume: 0.5 mL Serum
Specimen Preparation
Plain Red or Serum Separator Tube (SST)
Allow sample to clot fully for 30 minutes at room temperature
Centrifuge within 2 hours of collection
Transfer serum to plastic transport tube.
Samples may be frozen and thawed up to 3 times. Mix frozen samples well after thawing.
Storage/Transport Temperature
Frozen. Submit specimen in plastic transport tube.
Stability (from collection to initiation)
Ambient: 24 hours
Refrigerated: 7 days
Frozen: 1 month
Unacceptable Conditions
Hemolyzed, icteric, lipemic or bacterially contaminated sera.
Test Performance / Compliance
Performed
Monday - Friday
Reported
Same day
Performing Lab
Avera McKennan Regional Laboratory
CPT Codes
86039
Compliance & Service Center Alerts
ANA Screen Only. Does not include reflex testing. If reflex testing is clinically indicated, order ANA by IFA with Reflex to ENA.
Methodology
Indirect Immunofluorescence using Hep-2 cells
Clinical Information
Interpretive Data
ANA is a standard screening assay for detecting multiple autoantibodies produced by patients with an ANA associated rheumatic disease. In 2009 a task force of the American College of Rheumatology issued a statement declaring Hep-2 indirect immunofluorescence as the preferred method for ANA screening.
Avera McKennan Laboratory will report 5 patterns and respective titers: Homogeneous, Speckled, Centromere, Nucleolar and Nuclear Dots. Cytoplasmic staining will be noted if present.
