HUP: One FULL 2.7 light blue (sodium citrate) tube HUP54: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes PAH: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes
Collect
HUP & HUP54: One FULL 2.7ml light blue (sodium citrate) tube
PAH: Must be filled to minimum fill indicator line, no more than the maximum fill line to achieve the 9:1 blood: Na citrate ratio
Pediatric Collection
HUP: One FULL 1.8 ml light blue tube, invert 8-10 times *Please contact CHOP for pediatric tubes at 215-590-1775*
PAH: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes
Specimen Preparation
HUP: If delivery of specimen is likely to be delayed for more than 12 hours prepare platelet poor plasma by following these steps:
1. Spin the specimen at 2800 rpm for 10 minutes.
2. Transfer the plasma into a labeled plastic pour off tube and respin at 2800 rpm for 10 minutes
3. After second spin, transfer plasma into a plastic pour-off tube labeled properly with patient information and freeze.
PAH: Need platelet poor plasma if delayed more than 12hrs.
Storage/Transport Temperature
Transport: Room Temperature if less than 12 hours. If more than 12 hours send frozen plasma
Storage: Frozen: processed plasma if more than 12 hours
-70°C 12 months
-20°C 14 days
Stability (from collection to initiation)
Uncentifuged samples are stable at Room Temperature for 12 hours
Centrifuged samples are stable refrigerated or at room temperature for 12 hours
Unacceptable Conditions
HUP & HUP54: QNS (quantity not sufficient) or clotted specimens PAH: QNS,clotted, marked hemolysis
Reference Interval
Normal Ranges
PT: 9.4-12.5 seconds
INR: 08-1.1
Critical Values
INR: > 5.0
Testing Limitations
A number of prescription medications, over the-counter medications, herbal products, hormone replacement therapy, oral contraceptives, nonsteroidal anti-inflammatory drugs, Vitamin K, anticoagulant therapy and antibiotics can alter the INR.
RecombiPlasTin 2G sees no interference on the ACL TOP Family up to:
Clinical Significance
PT is an important screening test to detect the deficiency of one or more of the clotting factors of the Extrinsic pathway of the coagulation cascade. This deficiency may be hereditary or due to acquired causes such as liver disease, Vitamin K deficiency, or a specific factor inhibitor. Prolonged PT indicates deficiencies of plasma factors VII, X, V, II or fibrinogen, as well as inhibitors to these factors. PT is useful in monitoring oral anticoagulant therapy and maintaining a patient in a safe clinical range due to its sensitivity to decrease in Factors II, VII, and X typically observed with Warfarin.
CPT Codes
85610
LOINC
5902-2
6301-6
Collection & Processing
Collection Container
HUP: One FULL 2.7 light blue (sodium citrate) tube HUP54: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes PAH: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes
Collect
HUP & HUP54: One FULL 2.7ml light blue (sodium citrate) tube
PAH: Must be filled to minimum fill indicator line, no more than the maximum fill line to achieve the 9:1 blood: Na citrate ratio
Pediatric Collection
HUP: One FULL 1.8 ml light blue tube, invert 8-10 times *Please contact CHOP for pediatric tubes at 215-590-1775*
PAH: Plastic BD 2.7mL and 1.8mL 3.2% sodium citrate tubes
Specimen Preparation
HUP: If delivery of specimen is likely to be delayed for more than 12 hours prepare platelet poor plasma by following these steps:
1. Spin the specimen at 2800 rpm for 10 minutes.
2. Transfer the plasma into a labeled plastic pour off tube and respin at 2800 rpm for 10 minutes
3. After second spin, transfer plasma into a plastic pour-off tube labeled properly with patient information and freeze.
PAH: Need platelet poor plasma if delayed more than 12hrs.
Storage/Transport Temperature
Transport: Room Temperature if less than 12 hours. If more than 12 hours send frozen plasma
Storage: Frozen: processed plasma if more than 12 hours
-70°C 12 months
-20°C 14 days
Stability (from collection to initiation)
Uncentifuged samples are stable at Room Temperature for 12 hours
Centrifuged samples are stable refrigerated or at room temperature for 12 hours
Unacceptable Conditions
HUP & HUP54: QNS (quantity not sufficient) or clotted specimens PAH: QNS,clotted, marked hemolysis
CR&P Information
Result Interpretation
Reference Interval
Normal Ranges
PT: 9.4-12.5 seconds
INR: 08-1.1
Critical Values
INR: > 5.0
Testing Limitations
A number of prescription medications, over the-counter medications, herbal products, hormone replacement therapy, oral contraceptives, nonsteroidal anti-inflammatory drugs, Vitamin K, anticoagulant therapy and antibiotics can alter the INR.
RecombiPlasTin 2G sees no interference on the ACL TOP Family up to:
Clinical Significance
PT is an important screening test to detect the deficiency of one or more of the clotting factors of the Extrinsic pathway of the coagulation cascade. This deficiency may be hereditary or due to acquired causes such as liver disease, Vitamin K deficiency, or a specific factor inhibitor. Prolonged PT indicates deficiencies of plasma factors VII, X, V, II or fibrinogen, as well as inhibitors to these factors. PT is useful in monitoring oral anticoagulant therapy and maintaining a patient in a safe clinical range due to its sensitivity to decrease in Factors II, VII, and X typically observed with Warfarin.
Testing Updates
Billing Codes
CPT Codes
85610
LOINC
5902-2
6301-6
Ordering Information
Cerner Orderable
PT
Penn Chart Orderable
INR (aka PROTHROMBIN) [C0300092]
Performing Lab
Coagulation
Performed
Sunday-Saturday
Reported
Once Received in HUP Lab:
STAT: 60 mins
Routine: 2 hours
