Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease (all body fluids) or Neurology (CSF only). UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
Available Stat
No
Performing Lab
Microbiology
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Preferred Volume
2ml
Minimum Volume
1ml
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Test Code
P710
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reference Interval
No microorganisms detected
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease (all body fluids) or Neurology (CSF only). UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
Available Stat
No
Test Code
P710
Performing Lab
Microbiology
Methodology
mNGS
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Reference Interval
No microorganisms detected
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease (all body fluids) or Neurology (CSF only). UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
Available Stat
No
Performing Lab
Microbiology
Methodology
mNGS
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Preferred Volume
2ml
Minimum Volume
1ml
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Processing
Test Code
P710
Performing Lab
Microbiology
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Result Interpretation
Reference Interval
No microorganisms detected
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
Yes, testing of UCSF patients requires approval by Microbiology in consultation with Infectious Disease (all body fluids) or Neurology (CSF only). UCSF outpatient testing requires insurance preauthorization. Please follow this link for an insurance preauthorization template (Microsoft Word format).
Requests from outside UCSF require an institutional account prior to sample submission. See https://nextgendiagnostics.ucsf.edu for details.
Available Stat
No
Test Code
P710
Performing Lab
Microbiology
Methodology
mNGS
Collect
CSF: Sterile tube
Plasma: Lavender top tube. A dedicated tube is strongly preferred.
Sample Type
CSF, EDTA plasma (EDTA whole blood must be centrifuged and plasma separated from red cells within 6 hours of collection).
Preferred Volume
2ml
Minimum Volume
1ml
Unacceptable Conditions
Samples submitted in incorrect tube, grossly hemolyzed samples
Specimen Preparation
UCSF: Freeze at -70ºC on receipt at China Basin. Other sites: Freeze sample and ship to China Basin microbiology. Aliquot (if necessary) only under sterile conditions.
Reference Interval
No microorganisms detected
Synonyms
metagenomic next-generation sequencing
mNGS
agnostic sequencing
untargeted sequencing
Stability (from collection to initiation)
CSF: Room temperature 6 hours or refrigerated 6 days but prefer to freeze within 6 hours; stable frozen at -25C or -70C for up to 1 year.
Plasma: Room temperature up to 6 hours for whole blood prior to centrifugation; plasma refrigerated up to 7 days; stable frozen at -25C or -70C for up to 1 year.
Reported
1-2 weeks
Additional Information
Test results are interpreted by the Microbiology lab director and may be discussed with treating physicians as part of an ad hoc Clinical Microbial Sequencing Board (CMSB).
