Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
Sample Type
EDTA whole blood from mother of fetus. Should only be ordered in conjunction with prenatal genetic testing on either amniotic fluid or chorionic villi.
Collect
Lavender top (EDTA)
Amount to Collect
3 mL blood
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Remarks
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Unacceptable Conditions
Heparinized whole blood received.
Test Code
MCC
Performing Lab
Genomic Services - Molecular Diagnostics Lab
Specimen Preparation
Do not centrifuge the specimen. Store at room temperature. Refrigerated samples are acceptable
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Unacceptable Conditions
Heparinized whole blood received.
Reference Interval
Negative
Additional Information
Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
CPT Codes
81265
LDT or Modified FDA
Yes
LOINC Codes
35457-1
Available Stat
No
Ordering Recommendations
Test can be ordered in APeX
Test Code
MCC
Performing Lab
Genomic Services - Molecular Diagnostics Lab
Performed
Run 1x per week or as needed, day shift only
Methodology
PCR and capillary electrophoresis
Remarks
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Collect
Lavender top (EDTA)
Amount to Collect
3 mL blood
Sample Type
EDTA whole blood from mother of fetus. Should only be ordered in conjunction with prenatal genetic testing on either amniotic fluid or chorionic villi.
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Unacceptable Conditions
Heparinized whole blood received.
Specimen Preparation
Do not centrifuge the specimen. Store at room temperature. Refrigerated samples are acceptable
Reference Interval
Negative
Reported
10-14 days
Additional Information
Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
CPT Codes
81265
LDT or Modified FDA
Yes
LOINC Codes
35457-1
Ordering
Ordering Recommendations
Test can be ordered in APeX
Available Stat
No
Performing Lab
Genomic Services - Molecular Diagnostics Lab
Performed
Run 1x per week or as needed, day shift only
Methodology
PCR and capillary electrophoresis
Reported
10-14 days
Additional Information
Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
Collection
Sample Type
EDTA whole blood from mother of fetus. Should only be ordered in conjunction with prenatal genetic testing on either amniotic fluid or chorionic villi.
Collect
Lavender top (EDTA)
Amount to Collect
3 mL blood
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Remarks
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Unacceptable Conditions
Heparinized whole blood received.
Processing
Test Code
MCC
Performing Lab
Genomic Services - Molecular Diagnostics Lab
Specimen Preparation
Do not centrifuge the specimen. Store at room temperature. Refrigerated samples are acceptable
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Unacceptable Conditions
Heparinized whole blood received.
Result Interpretation
Reference Interval
Negative
Additional Information
Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
Administrative
CPT Codes
81265
LDT or Modified FDA
Yes
LOINC Codes
35457-1
Complete View
Available Stat
No
Ordering Recommendations
Test can be ordered in APeX
Test Code
MCC
Performing Lab
Genomic Services - Molecular Diagnostics Lab
Performed
Run 1x per week or as needed, day shift only
Methodology
PCR and capillary electrophoresis
Remarks
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Collect
Lavender top (EDTA)
Amount to Collect
3 mL blood
Sample Type
EDTA whole blood from mother of fetus. Should only be ordered in conjunction with prenatal genetic testing on either amniotic fluid or chorionic villi.
Preferred Volume
3 mL blood
Minimum Volume
1.5 mL blood
Unacceptable Conditions
Heparinized whole blood received.
Specimen Preparation
Do not centrifuge the specimen. Store at room temperature. Refrigerated samples are acceptable
Reference Interval
Negative
Reported
10-14 days
Additional Information
Contamination of prenatal amniotic fluid or chorionic villi samples with maternal cells or tissue may lead to misinterpretation of prenatal diagnostic tests, particularly in determination of true fetal heterozygosity. Maternal contamination of prenatal samples is assessed by genotyping maternal and fetal DNA at 15 different autosomal short-tandem repeats loci with heterozygosity rates ranging from 70-93%. This assay can detect maternal DNA contamination at approximately 10% of fetal DNA.
This test was developed, and its performance characteristics determined by the Clinical Laboratories at the Medical Center at UC San Francisco. It has not been cleared or approved by the U.S. FDA.
