The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Reflex Testing
An interpretation of this test by a laboratory physician will be automatically performed and billed for separately.
Synonyms
PTPN11 exons 3/4/13
NRAS exons 2/3
KRAS exons 2/3
CBL exons 8/9
JMML Panel
Supplemental Test Request Form Required
Yes
Sample Type
Tumor sample EDTA whole blood or Bone marrow aspirate
Germline sample Buccal swab or Cultured fibroblasts
Note: Buccal swabs are required for all new patients.(CCGL - 415-502-3252)
Collect
Lavender top (EDTA) Blood or bone marrow
Buccal swab or T25 flask Germline sample
Amount to Collect
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Remarks
Buccal swabs are required for new patients to determine the presence of a variant in normal vs. tumor samples. Wipe Cheek multiple times with cytobrush/cotton swabs. Transport in room temperature.
Avoid hemolysis. Due to stability issues these samples should only be collected at UCSF Monday through noon Friday.
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Test Code
JMML
Performing Lab
UCSF Clinical Cancer Genomics Lab
Specimen Preparation
DO NOT FREEZE blood or bone marrow samples. Ship samples to CCGL at Mt Zion as soon as possible.
Overnight refrigeration prior to transport is acceptable.
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Reference Interval
Negative: No clinically significant variants detected.
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Interpretive Data
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
CPT Codes
81450
LDT or Modified FDA
Yes
Available Stat
No
Test Code
JMML
Performing Lab
UCSF Clinical Cancer Genomics Lab
Performed
Run twice per week, day shift only
Methodology
Targeted next generation DNA sequencing (NovaSeq)
Remarks
Buccal swabs are required for new patients to determine the presence of a variant in normal vs. tumor samples. Wipe Cheek multiple times with cytobrush/cotton swabs. Transport in room temperature.
Avoid hemolysis. Due to stability issues these samples should only be collected at UCSF Monday through noon Friday.
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Collect
Lavender top (EDTA) Blood or bone marrow
Buccal swab or T25 flask Germline sample
Amount to Collect
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Sample Type
Tumor sample EDTA whole blood or Bone marrow aspirate
Germline sample Buccal swab or Cultured fibroblasts
Note: Buccal swabs are required for all new patients.(CCGL - 415-502-3252)
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Specimen Preparation
DO NOT FREEZE blood or bone marrow samples. Ship samples to CCGL at Mt Zion as soon as possible.
Overnight refrigeration prior to transport is acceptable.
Reference Interval
Negative: No clinically significant variants detected.
Interpretive Data
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Synonyms
JMML Panel
CBL exons 8/9
KRAS exons 2/3
NRAS exons 2/3
PTPN11 exons 3/4/13
Reported
14 days
Reflex Testing
An interpretation of this test by a laboratory physician will be automatically performed and billed for separately.
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Reflex Testing
An interpretation of this test by a laboratory physician will be automatically performed and billed for separately.
Synonyms
PTPN11 exons 3/4/13
NRAS exons 2/3
KRAS exons 2/3
CBL exons 8/9
JMML Panel
Supplemental Test Request Form Required
Yes
Collection
Sample Type
Tumor sample EDTA whole blood or Bone marrow aspirate
Germline sample Buccal swab or Cultured fibroblasts
Note: Buccal swabs are required for all new patients.(CCGL - 415-502-3252)
Collect
Lavender top (EDTA) Blood or bone marrow
Buccal swab or T25 flask Germline sample
Amount to Collect
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Remarks
Buccal swabs are required for new patients to determine the presence of a variant in normal vs. tumor samples. Wipe Cheek multiple times with cytobrush/cotton swabs. Transport in room temperature.
Avoid hemolysis. Due to stability issues these samples should only be collected at UCSF Monday through noon Friday.
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Processing
Test Code
JMML
Performing Lab
UCSF Clinical Cancer Genomics Lab
Specimen Preparation
DO NOT FREEZE blood or bone marrow samples. Ship samples to CCGL at Mt Zion as soon as possible.
Overnight refrigeration prior to transport is acceptable.
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Result Interpretation
Reference Interval
Negative: No clinically significant variants detected.
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Interpretive Data
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Administrative
CPT Codes
81450
LDT or Modified FDA
Yes
Complete View
Available Stat
No
Test Code
JMML
Performing Lab
UCSF Clinical Cancer Genomics Lab
Performed
Run twice per week, day shift only
Methodology
Targeted next generation DNA sequencing (NovaSeq)
Remarks
Buccal swabs are required for new patients to determine the presence of a variant in normal vs. tumor samples. Wipe Cheek multiple times with cytobrush/cotton swabs. Transport in room temperature.
Avoid hemolysis. Due to stability issues these samples should only be collected at UCSF Monday through noon Friday.
Do not collect sample in heparin. Keep sample refrigerated for overnight or longer storage.
Collect
Lavender top (EDTA) Blood or bone marrow
Buccal swab or T25 flask Germline sample
Amount to Collect
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Sample Type
Tumor sample EDTA whole blood or Bone marrow aspirate
Germline sample Buccal swab or Cultured fibroblasts
Note: Buccal swabs are required for all new patients.(CCGL - 415-502-3252)
Preferred Volume
Tumor sample
EDTA whole blood: 5 mL
Bone Marrow aspirate: 3 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 4-6 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Minimum Volume
Tumor sample
EDTA whole blood: 2 mL
Bone Marrow aspirate: 1 mL
Germline sample
Cultured fibroblasts: Confluent T25 flasks x2
Buccal swab: Collect 2-4 cytobrush/cotton swabs on each side of the mouth, place swabs in transport tubes, 2 swabs per tube.
Specimen Preparation
DO NOT FREEZE blood or bone marrow samples. Ship samples to CCGL at Mt Zion as soon as possible.
Overnight refrigeration prior to transport is acceptable.
Reference Interval
Negative: No clinically significant variants detected.
Interpretive Data
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
Synonyms
JMML Panel
CBL exons 8/9
KRAS exons 2/3
NRAS exons 2/3
PTPN11 exons 3/4/13
Reported
14 days
Reflex Testing
An interpretation of this test by a laboratory physician will be automatically performed and billed for separately.
The test was validated by the UCSF Clinical Cancer Genomics Laboratory (CCGL) to confirm performance characteristics, in compliance with current guidelines for clinical implementation.
