Run Monday-Friday AM with prior notice (excluding holidays)
Methodology
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Reported
Results available next day by 1300 hours (Monday through Friday, excluding holidays)
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
Synonyms
Busulfex
Sample Type
Heparinized plasma
Collect
Dark green top on ice drawn at specific times
Amount to Collect
2 mL blood for each sample
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Remarks
Testing should be ordered in APeX.
For PK studies on the first busulfan dose, the first sample should be drawn immediately after termination of the IV infusion. For PK studies on the steady state busulfan doses, the first sample should be drawn immediately before the start of the IV infusion. The remaining samples should be drawn at the times listed on the busulfan PK sheets (use correct PK sheet for each study: e.g. Q6hr, Q12hr or Q24hr).
Each patient sample should have a label affixed to it, and the EXACT time of collection should be written on the label or the APeX requisition and on the busulfan PK sheet. Hand-carry the sample to lab on ice with the requisition. Make sure to notify lab staff that you are delivering a busulfan sample, DO NOT simply leave it at the desk. Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing. With the final sample, bring the busulfan PK sheet with the correct collect times completed for each sample to the lab.
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label or ApeX requisition. Place a Sunquest label on the tube. Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Units
ng/mL
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
Run Monday-Friday AM with prior notice (excluding holidays)
Methodology
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Remarks
Testing should be ordered in APeX.
For PK studies on the first busulfan dose, the first sample should be drawn immediately after termination of the IV infusion. For PK studies on the steady state busulfan doses, the first sample should be drawn immediately before the start of the IV infusion. The remaining samples should be drawn at the times listed on the busulfan PK sheets (use correct PK sheet for each study: e.g. Q6hr, Q12hr or Q24hr).
Each patient sample should have a label affixed to it, and the EXACT time of collection should be written on the label or the APeX requisition and on the busulfan PK sheet. Hand-carry the sample to lab on ice with the requisition. Make sure to notify lab staff that you are delivering a busulfan sample, DO NOT simply leave it at the desk. Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing. With the final sample, bring the busulfan PK sheet with the correct collect times completed for each sample to the lab.
Collect
Dark green top on ice drawn at specific times
Amount to Collect
2 mL blood for each sample
Sample Type
Heparinized plasma
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Specimen Preparation
Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label or ApeX requisition. Place a Sunquest label on the tube. Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.
Units
ng/mL
Synonyms
Busulfex
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Reported
Results available next day by 1300 hours (Monday through Friday, excluding holidays)
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
CPT Codes
80299 x number of samples tested
LDT or Modified FDA
Yes
Ordering
Available Stat
No
Performing Lab
China Basin Chemistry
Performed
Run Monday-Friday AM with prior notice (excluding holidays)
Methodology
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Reported
Results available next day by 1300 hours (Monday through Friday, excluding holidays)
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
Synonyms
Busulfex
Collection
Sample Type
Heparinized plasma
Collect
Dark green top on ice drawn at specific times
Amount to Collect
2 mL blood for each sample
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Remarks
Testing should be ordered in APeX.
For PK studies on the first busulfan dose, the first sample should be drawn immediately after termination of the IV infusion. For PK studies on the steady state busulfan doses, the first sample should be drawn immediately before the start of the IV infusion. The remaining samples should be drawn at the times listed on the busulfan PK sheets (use correct PK sheet for each study: e.g. Q6hr, Q12hr or Q24hr).
Each patient sample should have a label affixed to it, and the EXACT time of collection should be written on the label or the APeX requisition and on the busulfan PK sheet. Hand-carry the sample to lab on ice with the requisition. Make sure to notify lab staff that you are delivering a busulfan sample, DO NOT simply leave it at the desk. Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing. With the final sample, bring the busulfan PK sheet with the correct collect times completed for each sample to the lab.
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label or ApeX requisition. Place a Sunquest label on the tube. Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Result Interpretation
Units
ng/mL
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
Run Monday-Friday AM with prior notice (excluding holidays)
Methodology
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Remarks
Testing should be ordered in APeX.
For PK studies on the first busulfan dose, the first sample should be drawn immediately after termination of the IV infusion. For PK studies on the steady state busulfan doses, the first sample should be drawn immediately before the start of the IV infusion. The remaining samples should be drawn at the times listed on the busulfan PK sheets (use correct PK sheet for each study: e.g. Q6hr, Q12hr or Q24hr).
Each patient sample should have a label affixed to it, and the EXACT time of collection should be written on the label or the APeX requisition and on the busulfan PK sheet. Hand-carry the sample to lab on ice with the requisition. Make sure to notify lab staff that you are delivering a busulfan sample, DO NOT simply leave it at the desk. Busulfan is unstable therefore EACH sample must be transported on ice to the laboratory immediately after collection for processing and freezing. With the final sample, bring the busulfan PK sheet with the correct collect times completed for each sample to the lab.
Collect
Dark green top on ice drawn at specific times
Amount to Collect
2 mL blood for each sample
Sample Type
Heparinized plasma
Preferred Volume
1 ml heparinized plasma for each sample
Minimum Volume
1 ml heparinized plasma for each sample
Unacceptable Conditions
Sample not received on ice. Not collected in Dark green top tube.
Specimen Preparation
Samples must be processed immediately upon receipt. When the samples arrive, receive them in Sunquest with the EXACT collection time as hand-written on the label or ApeX requisition. Place a Sunquest label on the tube. Spin sample down immediately in refrigerated centrifuge, aliquot 1 mL into plastic vial and label with the exact time of collection then freeze at -70C.
As additional samples arrive receive them in Sunquest under Order/Receipt Modify. Then process each sample as above. When all samples have been processed, send them on dry ice to China Basin Chemistry.
Units
ng/mL
Synonyms
Busulfex
Stability (from collection to initiation)
Before separation from cells: Refrigerated (4C) for 24 hours.
After separation from cells: Frozen (-70C) for 2 years.
Reported
Results available next day by 1300 hours (Monday through Friday, excluding holidays)
Additional Information
Chemistry China Basin will report the busulfan concentration of each sample.
For questions contact China Basin Chemistry at 415 353 4820
