Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
Reflex Testing
If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.
Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
Synonyms
AV 18/XY
Direct aneuploidy FISH 18/XY
A1321
BA1321
Sample Type
Heparinized whole blood, Amniotic fluid, CVS, POC
Collect
Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Stability (from collection to initiation)
1-2 days
Test Code
BA1321: Blood
A1321: Amniotic fluid, CVS
Test Group
FISH
Performing Lab
Medical genomics - Cytogenetics
Specimen Preparation
Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Stability (from collection to initiation)
1-2 days
Additional Information
Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
CPT Codes
88271 x2, 88275, 88291
LDT or Modified FDA
Yes
Available Stat
No
Test Code
BA1321: Blood
A1321: Amniotic fluid, CVS
Test Group
FISH
Performing Lab
Medical genomics - Cytogenetics
Performed
Set up daily, Monday - Friday
Methodology
Fluorescent in-situ hybridization (FISH)
Collect
Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)
Sample Type
Heparinized whole blood, Amniotic fluid, CVS, POC
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Specimen Preparation
Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Synonyms
AV 18/XY
Direct aneuploidy FISH 18/XY
A1321
BA1321
Stability (from collection to initiation)
1-2 days
Reported
2-3 days
Reflex Testing
If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.
Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
Additional Information
Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
CPT Codes
88271 x2, 88275, 88291
LDT or Modified FDA
Yes
Ordering
Available Stat
No
Performing Lab
Medical genomics - Cytogenetics
Performed
Set up daily, Monday - Friday
Methodology
Fluorescent in-situ hybridization (FISH)
Reported
2-3 days
Additional Information
Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
Reflex Testing
If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.
Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
Synonyms
AV 18/XY
Direct aneuploidy FISH 18/XY
A1321
BA1321
Collection
Sample Type
Heparinized whole blood, Amniotic fluid, CVS, POC
Collect
Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Stability (from collection to initiation)
1-2 days
Processing
Test Code
BA1321: Blood
A1321: Amniotic fluid, CVS
Test Group
FISH
Performing Lab
Medical genomics - Cytogenetics
Specimen Preparation
Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Stability (from collection to initiation)
1-2 days
Result Interpretation
Additional Information
Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
Administrative
CPT Codes
88271 x2, 88275, 88291
LDT or Modified FDA
Yes
Complete View
Available Stat
No
Test Code
BA1321: Blood
A1321: Amniotic fluid, CVS
Test Group
FISH
Performing Lab
Medical genomics - Cytogenetics
Performed
Set up daily, Monday - Friday
Methodology
Fluorescent in-situ hybridization (FISH)
Collect
Blood: Dark green top
Amniotic fluid: Sterile screw top container
CVS or POC: 15 mL centrifuge tube with transport media (Avaialable from Cytogenetics: 353-4844)
Sample Type
Heparinized whole blood, Amniotic fluid, CVS, POC
Preferred Volume
Whole blood, child or adult: 5 mL
Whole blood, infant: 2 mL
Amniotic fluid: 10 mL
CVS: 10 mg
POC: 10 mg
Minimum Volume
Whole blood, child or adult: 2 mL
Whole blood, infant: 1 mL
Amniotic fluid: 5 mL
CVS: 5 mg
POC: 5 mg
Specimen Preparation
Keep samples at Room temperature. DO NOT CENTRIFUGE for any reason. Send all tubes and completed paperwork asap to the Cytogenetics laboratory at China Basin.
Synonyms
AV 18/XY
Direct aneuploidy FISH 18/XY
A1321
BA1321
Stability (from collection to initiation)
1-2 days
Reported
2-3 days
Reflex Testing
If an abnormality is detected the Director will determine the appropriate additional studies to be performed to characterize the abnormality.
Additional testing may be omitted if specifically requested when the sample is submitted for cytogenetic analysis.
Additional Information
Direct FISH is limited to the detection of aneuploidy (increase or decrease in copy number) of chromosomes 13, 18, 21, X and Y by analyzing INTERPHASE nuclei. A normal result indicates that no numeric abnormality of chromosomes 13, 18, 21, X and Y were identified. It does not rule out the possibility of structural defects or numeric defects in other chromosomes.
DIRECT FISH results are preliminary: G-banded chromosome analysis of 15 colonies or 20 cells remains the standard of care for prenatal diagnosis and all cytogenetic studies. It is also standard of care that no irreversible therapeutic action be initiated on the basis of DIRECT FISH results alone. About one third of all chromosome abnormalities cannot be detected by DIRECT FISH, including structural abnormalities, mosaicism, and numerical abnormalities of other chromosomes.
"Direct FISH" is the UCSF Cytogenetics name for interphase fluorescence in situ hybridization (FISH) analysis for chromosomes 13, 18, 21, X, and Y.
Each FISH test is developed and its performance characteristics determined by the UCSF Cytogenetics Laboratory as required by CLIA '88 regulations. It has not been cleared or approved for specific uses by the U.S. Food and Drug Administration. All FISH probes undergo internal validation and quality control testing at UCSF Cytogenetics Lab prior to use.
