Detection monoclonal T-cell population in T-cell neoplasms
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days from receipt of specimen in performing lab
Methodology
The TCRG Gene Clonality Assay uses two multiplex master mixes that target conserved regions within the variable (V) and the joining (J) regions that flank the unique hypervariable antigen-binding region 3 (CDR3).
Use
TCR gene rearrangement is a useful marker of the clonality of T-lymphoid neoplasms
Limitations
Rearrangement will not be detected if the submitted tissue is not from the involved tumor. Tissue samples that are too small or fixed in formalin will not yield enough DNA to do an accurate analysis. Some rearrangement may not be detectable due to the nature of the breakpoints. Some B-cell malignancies will show T-cell receptor gene rearrangements.
Additional Information
TCR beta will be performed if TCR gamma is negative
Specimen Type
Whole Blood Bone Marrow Fresh Frozen Tissue FFPE Slides
Container
Lavender/EDTA Sterile container
Collection Instructions
Tissue must be carefully cut from the surgically removed tumor and contain a representative sampling of at least 5% to 10% of tumor cells from the involved area.
Volume
Whole Blood - 4 mL Bone Marrow - 4 mL Fresh Frozen Tissue - 5mm3 FFPE Slides - 10 Unstained Slides + 1 H&E Slide
Minimum Volume
Whole Blood - 1 mL Bone Marrow - 1 mL
Storage Temperature
Fresh frozen tissue can be stored in a 70°C freezer before transporting to the lab
Special Instructions
When TCR gamma is tested netgative will proceed to test TCR beta
Shipping and Handling Instructions
Blood and bone marrow transport at room temperature. Fresh frozen tissue transport on dry ice.
Causes for Rejection
Frozen tissue samples thawed during transit cannot be used for DNA analysis clotted specimem mislabelled specimen Broken tube
Reference Range
No clonality observed
Test Information
CPT Codes
81342 81340
Synonyms
T-Lymphocyte Receptor (TCR) Gene Rearrangement
Test Includes
Detection monoclonal T-cell population in T-cell neoplasms
Performing Laboratory / Facility
UCLA Medical Center Clinical Laboratory (CHS)
Performing Section
Molecular Pathology
Availability
Monday through Friday, 0700-1700
Turnaround Time
14 days from receipt of specimen in performing lab
Methodology
The TCRG Gene Clonality Assay uses two multiplex master mixes that target conserved regions within the variable (V) and the joining (J) regions that flank the unique hypervariable antigen-binding region 3 (CDR3).
Use
TCR gene rearrangement is a useful marker of the clonality of T-lymphoid neoplasms
Limitations
Rearrangement will not be detected if the submitted tissue is not from the involved tumor. Tissue samples that are too small or fixed in formalin will not yield enough DNA to do an accurate analysis. Some rearrangement may not be detectable due to the nature of the breakpoints. Some B-cell malignancies will show T-cell receptor gene rearrangements.
Additional Information
TCR beta will be performed if TCR gamma is negative
Specimen Collection and Handling
Specimen Type
Whole Blood Bone Marrow Fresh Frozen Tissue FFPE Slides
Container
Lavender/EDTA Sterile container
Collection Instructions
Tissue must be carefully cut from the surgically removed tumor and contain a representative sampling of at least 5% to 10% of tumor cells from the involved area.
Volume
Whole Blood - 4 mL Bone Marrow - 4 mL Fresh Frozen Tissue - 5mm3 FFPE Slides - 10 Unstained Slides + 1 H&E Slide
Minimum Volume
Whole Blood - 1 mL Bone Marrow - 1 mL
Storage Temperature
Fresh frozen tissue can be stored in a 70°C freezer before transporting to the lab
Special Instructions
When TCR gamma is tested netgative will proceed to test TCR beta
Shipping and Handling Instructions
Blood and bone marrow transport at room temperature. Fresh frozen tissue transport on dry ice.
Causes for Rejection
Frozen tissue samples thawed during transit cannot be used for DNA analysis clotted specimem mislabelled specimen Broken tube
