LAB6824 Anti-Angiotensin Type 1 Receptor (AT1R) LAB6833 Anti-Angiotensin Type 1 Receptor, Kidney
CPT Codes
86316
Synonyms
HLA
AT1R
Test Includes
Detection of antibodies to Angiotensin II Type I Receptors in human serum and plasma. AT1R is found in most major organs, suggesting the importance of anti-AT1R antibodies as a possible biomarker for solid organ transplants and Antibody Mediated Rejection (AMR).
Performing Laboratory / Facility
UCLA Immunogenetics Center
Performing Section
Immunogenetics
Availability
Monday through Friday, 0800-1700
Turnaround Time
5 days
Methodology
Antibodies to AT1R are identified in an ELISA based platform.
Use
Organ transplant candidates. Angiotensin II type I receptor II (AT1R) is a G protein-coupled receptor that mediates angiotensin effects and causes vasoconstriction in vascular smooth muscle cells, aldosterone secretion by the adrenal cortex and sodium reabsorption in proximal tubules. Binding of antibodies to AT1R mimics angiotensin II binding, results in agonistic activation of AT1R signaling pathways, and contributes to hypertension. Recent studies found a significant association of anti-AT1R antibodies, both pre- and post- transplantation, with graft rejection and failure in kidney and heart transplantation.
Patient Preparation
Draw specimen before hemodialysis of patient
Specimen Type
Blood
Container
Red
Collection Instructions
Deliver to Specimen Processing.
Volume
10 mL blood
Minimum Volume
Adult: 3 mL (1.5 mL serum) Pediatric: 1 mL (0.5 mL serum)
Stability
Keep blood at room temperature and transport samples as soon as possible after collection.
Shipping and Handling Instructions
Store at room temperature
Causes for Rejection
Specimen grossly hemolyzed Improper labeling of tubes or requisition
Reference Range
At a serum dilution of 1:40 a result >10 U/ml indicates risk of endothelial cell dysfunction. A sera with AT1R levels >40U/ml can be diluted to determine the titer of the antibody.
Test Information
Orderable Test Codes
LAB6824 Anti-Angiotensin Type 1 Receptor (AT1R) LAB6833 Anti-Angiotensin Type 1 Receptor, Kidney
CPT Codes
86316
Synonyms
HLA
AT1R
Test Includes
Detection of antibodies to Angiotensin II Type I Receptors in human serum and plasma. AT1R is found in most major organs, suggesting the importance of anti-AT1R antibodies as a possible biomarker for solid organ transplants and Antibody Mediated Rejection (AMR).
Performing Laboratory / Facility
UCLA Immunogenetics Center
Performing Section
Immunogenetics
Availability
Monday through Friday, 0800-1700
Turnaround Time
5 days
Methodology
Antibodies to AT1R are identified in an ELISA based platform.
Use
Organ transplant candidates. Angiotensin II type I receptor II (AT1R) is a G protein-coupled receptor that mediates angiotensin effects and causes vasoconstriction in vascular smooth muscle cells, aldosterone secretion by the adrenal cortex and sodium reabsorption in proximal tubules. Binding of antibodies to AT1R mimics angiotensin II binding, results in agonistic activation of AT1R signaling pathways, and contributes to hypertension. Recent studies found a significant association of anti-AT1R antibodies, both pre- and post- transplantation, with graft rejection and failure in kidney and heart transplantation.
Patient Preparation
Draw specimen before hemodialysis of patient
Specimen Collection and Handling
Specimen Type
Blood
Container
Red
Collection Instructions
Deliver to Specimen Processing.
Volume
10 mL blood
Minimum Volume
Adult: 3 mL (1.5 mL serum) Pediatric: 1 mL (0.5 mL serum)
Stability
Keep blood at room temperature and transport samples as soon as possible after collection.
Shipping and Handling Instructions
Store at room temperature
Causes for Rejection
Specimen grossly hemolyzed Improper labeling of tubes or requisition
Result Interpretation
Reference Range
At a serum dilution of 1:40 a result >10 U/ml indicates risk of endothelial cell dysfunction. A sera with AT1R levels >40U/ml can be diluted to determine the titer of the antibody.
