Peripheral blood lymphocytes that have previously been exposed in vivo to antigens become sensitized. If they are subsequently exposed in vitro to those same antigens, they will be triggered to initiate an immune response that culminates in proliferation. This assay uses the antigens Candida Albicans and tetanus toxoid to stimulate a purified mononuclear cell preparation and test for the ability of the cells to respond by proliferating. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. Cells undergoing DNA synthesis will incorporate the thymidine, which can then be measured using a microplate scintillation counter.
CPT Codes
86353x2
Collection
Collect
Blood in 4mL DkGrn NaHep (2 tubes needed). Collect only Mon-Fri. On Fridays, must be received to Burnet lab by 1500
Minimum Collection Volume
Contact Lab for minimum volumes (based on ALC)
Shipping/Handling Instructions (Lab Use Only)
Do not spin. Critical: Room Temp
External Client Shipping and Handling
Room temperature only. Must be received in lab within 24 hours of collection.
Peripheral blood lymphocytes that have previously been exposed in vivo to antigens become sensitized. If they are subsequently exposed in vitro to those same antigens, they will be triggered to initiate an immune response that culminates in proliferation. This assay uses the antigens Candida Albicans and tetanus toxoid to stimulate a purified mononuclear cell preparation and test for the ability of the cells to respond by proliferating. An excess of radio-labeled thymidine is made available to the cells during the later part of the culture period. Cells undergoing DNA synthesis will incorporate the thymidine, which can then be measured using a microplate scintillation counter.
